Screening for cold tolerance genes in C. elegans, whose expressions are affected by anticancer drugs camptothecin and leptomycin B

Okahata, Misaki; Sawada, Natsumi; Nakao, Kenji; Ohta, Akane; Kuhara, Atsushi

doi:https://doi.org/10.1038/s41598-024-55794-z

Screening for cold tolerance genes in C. elegans, whose expressions are affected by anticancer drugs camptothecin and leptomycin B

Okahata, Misaki; Sawada, Natsumi; Nakao, Kenji et al.

Scientific Reports, 2024, 14, 5401

アクセス数:575件（2026-07-28 07:07 集計）

固定URL: https://hdl.handle.net/11094/95640

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タイトル	Screening for cold tolerance genes in C. elegans, whose expressions are affected by anticancer drugs camptothecin and leptomycin B
著者	NRID 1000020880561 1000020880561 researchmap permalink mokahata mokahata Scopus Author ID 57189759584 57189759584 著者名 Okahata, Misaki
	著者名 Sawada, Natsumi
	著者名 Nakao, Kenji
	NRID 1000050410717 1000050410717 著者名 Ohta, Akane
	NRID 1000000402412 1000000402412 著者名 Kuhara, Atsushi
キーワード等	Caenorhabditis elegans
	Camptothecin
	Cold tolerance
	Leptomycin B
内容	Okahata M., Sawada N., Nakao K., et al. Screening for cold tolerance genes in C. elegans, whose expressions are affected by anticancer drugs camptothecin and leptomycin B. Scientific Reports 14, 5401 (2024); https://doi.org/10.1038/s41598-024-55794-z.
抄録	Temperature is a vital environmental factor affecting organisms’ survival as they determine the mechanisms to tolerate rapid temperature changes. We demonstrate an experimental system for screening chemicals that affect cold tolerance in Caenorhabditis elegans. The anticancer drugs leptomycin B and camptothecin were among the 4000 chemicals that were screened as those affecting cold tolerance. Genes whose expression was affected by leptomycin B or camptothecin under cold stimuli were investigated by transcriptome analysis. Abnormal cold tolerance was detected in several mutants possessing genes that were rendered defective and whose expression altered after exposure to either leptomycin B or camptothecin. The genetic epistasis analysis revealed that leptomycin B or camptothecin may increase cold tolerance by affecting a pathway upstream of the insulin receptor DAF-2 that regulates cold tolerance in the intestine. Our experimental system combining drug and cold tolerance could be used for a comprehensive screening of genes that control cold tolerance at a low cost and in a short time period.
出版者	Nature Research
収録物名	Scientific Reports
巻(号)	14
ページ	5401
刊行年月	2024-03-05
言語	英語
ハンドルURL	https://hdl.handle.net/11094/95640
eISSN	20452322
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41598-024-55794-z
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Scientific Reports. 2024, 14, p. 5401
DC.title	Screening for cold tolerance genes in C. elegans, whose expressions are affected by anticancer drugs camptothecin and leptomycin B
DC.creator	Okahata, Misaki
	Sawada, Natsumi
	Nakao, Kenji
	Ohta, Akane
	Kuhara, Atsushi
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-03-05
DC.identifier	https://doi.org/10.1038/s41598-024-55794-z
DC.subject	Caenorhabditis elegans
	Camptothecin
	Cold tolerance
	Leptomycin B
DC.description	Okahata M., Sawada N., Nakao K., et al. Screening for cold tolerance genes in C. elegans, whose expressions are affected by anticancer drugs camptothecin and leptomycin B. Scientific Reports 14, 5401 (2024); https://doi.org/10.1038/s41598-024-55794-z.
DCTERMS.abstract	Temperature is a vital environmental factor affecting organisms’ survival as they determine the mechanisms to tolerate rapid temperature changes. We demonstrate an experimental system for screening chemicals that affect cold tolerance in Caenorhabditis elegans. The anticancer drugs leptomycin B and camptothecin were among the 4000 chemicals that were screened as those affecting cold tolerance. Genes whose expression was affected by leptomycin B or camptothecin under cold stimuli were investigated by transcriptome analysis. Abnormal cold tolerance was detected in several mutants possessing genes that were rendered defective and whose expression altered after exposure to either leptomycin B or camptothecin. The genetic epistasis analysis revealed that leptomycin B or camptothecin may increase cold tolerance by affecting a pathway upstream of the insulin receptor DAF-2 that regulates cold tolerance in the intestine. Our experimental system combining drug and cold tolerance could be used for a comprehensive screening of genes that control cold tolerance at a low cost and in a short time period.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	95640
citation_title	Screening for cold tolerance genes in C. elegans, whose expressions are affected by anticancer drugs camptothecin and leptomycin B
citation_author	Okahata, Misaki
	Sawada, Natsumi
	Nakao, Kenji
	Ohta, Akane
	Kuhara, Atsushi
citation_publisher	Nature Research
citation_language	英語
citation_date	2024-03-05
citation_journal_title	Scientific Reports
citation_volume	14
citation_firstpage	5401
citation_doi	https://doi.org/10.1038/s41598-024-55794-z
citation_public_url	https://hdl.handle.net/11094/95640

Screening for cold tolerance genes in C. elegans, whose expressions are affected by anticancer drugs camptothecin and leptomycin B

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