Improvement of the Green-Red Förster Resonance Energy Transfer-Based Ca²⁺ Indicator by Using the Green Fluorescent Protein, Gamillus, with a Trans Chromophore as the Donor

Matsuda, Tomoki; Sakai, Shinya; Okazaki, Kei-Ichi et al.

ACS Sensors, 2024, 9(4), 1743-1748

アクセス数:541件（2025-08-17 06:45 集計）

固定URL: https://hdl.handle.net/11094/95641

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タイトル
Improvement of the Green-Red Förster Resonance Energy Transfer-Based Ca²⁺ Indicator by Using the Green Fluorescent Protein, Gamillus, with a Trans Chromophore as the Donor
著者
NRID
1000050419206 1000050419206
researchmap permalink
tikimaz tikimaz
Scopus Author ID
7404349300 7404349300
著者名
Matsuda, Tomoki
著者名
Sakai, Shinya
NRID
1000050792529 1000050792529
著者名
Okazaki, Kei-Ichi
NRID
1000020311350 1000020311350
ORCID
0000-0003-2650-9895 0000-0003-2650-9895
researchmap permalink
ng1 ng1
Scopus Author ID
7403433377 7403433377
著者名
Nagai, Takeharu
キーワード等
fluorescent indicator
fluorescent protein
Förster resonance energy transfer
genetically encoded Ca2+ indicators
protein engineering
内容
Matsuda T., Sakai S., Okazaki K.I., et al. Improvement of the Green-Red Förster Resonance Energy Transfer-Based Ca²⁺ Indicator by Using the Green Fluorescent Protein, Gamillus, with a Trans Chromophore as the Donor. ACS Sensors 9, 1743 (2024); https://doi.org/10.1021/acssensors.3c02398.
抄録
To monitor the Ca²⁺ dynamics in cells, various genetically encoded Ca²⁺ indicators (GECIs) based on Förster resonance energy transfer (FRET) between fluorescent proteins are widely used for live imaging. Conventionally, cyan and yellow fluorescent proteins have been often used as FRET pairs. Meanwhile, bathochromically shifted indicators with green and red fluorescent protein pairs have various advantages, such as low toxicity and autofluorescence in cells. However, it remains difficult to develop them with a similar level of dynamic range as cyan and yellow fluorescent protein pairs. To improve this, we used Gamillus, which has a unique trans-configuration chromophore, as a green fluorescent protein. Based on one of the best high-dynamic-range GECIs, Twitch-NR, we developed a GECI with 1.5-times higher dynamic range (253%), Twitch-GmRR, using RRvT as a red fluorescent protein. Twitch-GmRR had high brightness and photostability and was successfully applied for imaging the Ca²⁺ dynamics in live cells. Our results suggest that Gamillus with trans-type chromophores contributes to improving the dynamic range of GECIs. Therefore, selection of the cis-trans isomer of the chromophore may be a fundamental approach to improve the dynamic range of green-red FRET indicators, unlimited by GECIs.
出版者
American Chemical Society
収録物名
ACS Sensors
巻(号)
9(4)
ページ
1743-1748
刊行年月
2024-04-26
言語
英語
ハンドルURL
https://hdl.handle.net/11094/95641
eISSN
23793694
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1021/acssensors.3c02398
アクセス権
open access
権利情報
This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article