Dynamics of Wnt/β-catenin reporter activity throughout whole life in a naturally short-lived vertebrate

Ogamino, Shohei; Yamamichi, Moeko; Sato, Ken; Ishitani, Tohru

doi:https://doi.org/10.1038/s41514-024-00149-1

Dynamics of Wnt/β-catenin reporter activity throughout whole life in a naturally short-lived vertebrate

Ogamino, Shohei; Yamamichi, Moeko; Sato, Ken et al.

npj Aging, 2024, 10, 23

アクセス数:328件（2025-07-22 20:57 集計）

固定URL: https://hdl.handle.net/11094/95778

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タイトル	Dynamics of Wnt/β-catenin reporter activity throughout whole life in a naturally short-lived vertebrate
著者	著者名 Ogamino, Shohei
	著者名 Yamamichi, Moeko
	NRID 1000040396619 1000040396619 著者名 Sato, Ken
	NRID 1000040448428 1000040448428 ORCID 0000-0002-6200-7043 0000-0002-6200-7043 researchmap permalink read0148831 read0148831 Scopus Author ID 8079317200 8079317200 著者名 Ishitani, Tohru
内容	Ogamino S., Yamamichi M., Sato K., et al. Dynamics of Wnt/β-catenin reporter activity throughout whole life in a naturally short-lived vertebrate. npj Aging 10, 23 (2024); https://doi.org/10.1038/s41514-024-00149-1.
抄録	Wnt/β-catenin signaling plays a major role in regulation of embryogenesis, organogenesis, and adult tissue homeostasis and regeneration. However, the roles played by Wnt/β-catenin and the spatiotemporal regulation of its activity throughout life, including during aging, are not fully understood. To address these issues, we introduced a Wnt/β-catenin signaling sensitive reporter into African turquoise killifish (Nothobranchius furzeri), a naturally ultra-short-lived fish that allows for the analysis of its whole life within a short period of time. Using this reporter killifish, we unraveled the previously unidentified dynamics of Wnt/β-catenin signaling during development and aging. Using the reporter strain, we detected Wnt/β-catenin activity in actively developing tissues as reported in previous reports, but also observed activation and attenuation of Wnt/β-catenin activity during embryonic reaggregation and diapause, respectively. During the aging process, the reporter was activated in the choroidal layer and liver, but its expression decreased in the kidneys. In addition, the reporter also revealed that aging disrupts the spatial regulation and intensity control of Wnt/β-catenin activity seen during fin regeneration, which interferes with precise regeneration. Thus, the employed reporter killifish is a highly useful model for investigating the dynamics of Wnt/β-catenin signaling during both the developmental and aging process.
出版者	Nature Research
収録物名	npj Aging
巻(号)	10
ページ	23
刊行年月	2024-04-29
言語	英語
ハンドルURL	https://hdl.handle.net/11094/95778
eISSN	27316068
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41514-024-00149-1
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	npj Aging. 2024, 10, p. 23
DC.title	Dynamics of Wnt/β-catenin reporter activity throughout whole life in a naturally short-lived vertebrate
DC.creator	Ogamino, Shohei
	Yamamichi, Moeko
	Sato, Ken
	Ishitani, Tohru
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-04-29
DC.identifier	https://doi.org/10.1038/s41514-024-00149-1
DC.description	Ogamino S., Yamamichi M., Sato K., et al. Dynamics of Wnt/β-catenin reporter activity throughout whole life in a naturally short-lived vertebrate. npj Aging 10, 23 (2024); https://doi.org/10.1038/s41514-024-00149-1.
DCTERMS.abstract	Wnt/β-catenin signaling plays a major role in regulation of embryogenesis, organogenesis, and adult tissue homeostasis and regeneration. However, the roles played by Wnt/β-catenin and the spatiotemporal regulation of its activity throughout life, including during aging, are not fully understood. To address these issues, we introduced a Wnt/β-catenin signaling sensitive reporter into African turquoise killifish (Nothobranchius furzeri), a naturally ultra-short-lived fish that allows for the analysis of its whole life within a short period of time. Using this reporter killifish, we unraveled the previously unidentified dynamics of Wnt/β-catenin signaling during development and aging. Using the reporter strain, we detected Wnt/β-catenin activity in actively developing tissues as reported in previous reports, but also observed activation and attenuation of Wnt/β-catenin activity during embryonic reaggregation and diapause, respectively. During the aging process, the reporter was activated in the choroidal layer and liver, but its expression decreased in the kidneys. In addition, the reporter also revealed that aging disrupts the spatial regulation and intensity control of Wnt/β-catenin activity seen during fin regeneration, which interferes with precise regeneration. Thus, the employed reporter killifish is a highly useful model for investigating the dynamics of Wnt/β-catenin signaling during both the developmental and aging process.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	95778
citation_title	Dynamics of Wnt/β-catenin reporter activity throughout whole life in a naturally short-lived vertebrate
citation_author	Ogamino, Shohei
	Yamamichi, Moeko
	Sato, Ken
	Ishitani, Tohru
citation_publisher	Nature Research
citation_language	英語
citation_date	2024-04-29
citation_journal_title	npj Aging
citation_volume	10
citation_firstpage	23
citation_doi	https://doi.org/10.1038/s41514-024-00149-1
citation_public_url	https://hdl.handle.net/11094/95778

Dynamics of Wnt/β-catenin reporter activity throughout whole life in a naturally short-lived vertebrate

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