Potential of activated sludge-derived mixed microbial culture enriched on acetate to produce polyhydroxyalkanoates from various substrates

Ren, Yu; Inoue, Daisuke; Ike, Michihiko

doi:https://doi.org/10.1007/s10163-024-01974-y

Potential of activated sludge-derived mixed microbial culture enriched on acetate to produce polyhydroxyalkanoates from various substrates

Ren, Yu; Inoue, Daisuke; Ike, Michihiko

Journal of Material Cycles and Waste Management, 2024, 26, 2355-2365

アクセス数:270件（2025-07-06 08:06 集計）

固定URL: https://hdl.handle.net/11094/97121

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タイトル	Potential of activated sludge-derived mixed microbial culture enriched on acetate to produce polyhydroxyalkanoates from various substrates
著者	著者名 Ren, Yu
	NRID 1000070448091 1000070448091 ORCID 0000-0002-9216-8198 0000-0002-9216-8198 researchmap permalink DaisukeINOUE DaisukeINOUE Scopus Author ID 55723196300 55723196300 著者名 Inoue, Daisuke
	NRID 1000040222856 1000040222856 ORCID 0000-0001-6237-8103 0000-0001-6237-8103 researchmap permalink ikemichi ikemichi Scopus Author ID 55551254800 55551254800 著者名 Ike, Michihiko
キーワード等	Activated sludge
	Aerobic dynamic discharge process
	Mixed microbial culture
	Polyhydroxyalkanoates
	Substrate versatility
内容	The version of record of this article, first published in Journal of Material Cycles and Waste Management, is available online at Publisher’s website: https://doi.org/10.1007/s10163-024-01974-y.
抄録	The use of waste activated sludge (WAS) as a biocatalyst to produce polyhydroxyalkanoates (PHA) from waste streams may help promote the beneficial use of WAS for low-carbon, sustainable wastewater treatment. However, it remains unclear which types of substrates can be used for efficient PHA production, and how the PHA production can be maximized. This study aimed to assess the substrate versatility of mixed microbial cultures (MMCs) constructed from WAS by enriching PHA-accumulating bacteria using an aerobic dynamic discharge (ADD) process fed with acetate. Twelve different substrates, including organic acids, saccharides, and alcohols, were selected as the test substrates. In single-batch assays, the highest PHA production (583–680 mg/L) was achieved using butyrate, acetate, and pyruvate. In fed-batch assays, > 30 wt% PHA content was achieved using acetate, butyrate, propionate, lactate, and ethanol, with the highest content (60.3 wt%) using acetate. These results indicate that acetate-fed MMC by the ADD process could efficiently produce PHA from volatile fatty acids, lactate, pyruvate, and ethanol. Polyhydroxybutyrate was preferentially produced from acetate, butyrate, pyruvate, lactate, and ethanol, whereas polyhydroxyvalerate was notably produced from propionate. The results suggest that PHA can be efficiently produced from a wide range of substrates using MMCs enriched on a single substrate.
出版者	Springer
収録物名	Journal of Material Cycles and Waste Management
巻(号)	26
ページ	2355-2365
刊行年月	2024-01-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/97121
PISSN	14384957
eISSN	16118227
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1007/s10163-024-01974-y
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Journal of Material Cycles and Waste Management. 2024, 26, p. 2355-2365
DC.title	Potential of activated sludge-derived mixed microbial culture enriched on acetate to produce polyhydroxyalkanoates from various substrates
DC.creator	Ren, Yu
	Inoue, Daisuke
	Ike, Michihiko
DC.publisher	Springer
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-01-01
DC.identifier	https://doi.org/10.1007/s10163-024-01974-y
DC.subject	Activated sludge
	Aerobic dynamic discharge process
	Mixed microbial culture
	Polyhydroxyalkanoates
	Substrate versatility
DC.description	The version of record of this article, first published in Journal of Material Cycles and Waste Management, is available online at Publisher’s website: https://doi.org/10.1007/s10163-024-01974-y.
DCTERMS.abstract	The use of waste activated sludge (WAS) as a biocatalyst to produce polyhydroxyalkanoates (PHA) from waste streams may help promote the beneficial use of WAS for low-carbon, sustainable wastewater treatment. However, it remains unclear which types of substrates can be used for efficient PHA production, and how the PHA production can be maximized. This study aimed to assess the substrate versatility of mixed microbial cultures (MMCs) constructed from WAS by enriching PHA-accumulating bacteria using an aerobic dynamic discharge (ADD) process fed with acetate. Twelve different substrates, including organic acids, saccharides, and alcohols, were selected as the test substrates. In single-batch assays, the highest PHA production (583–680 mg/L) was achieved using butyrate, acetate, and pyruvate. In fed-batch assays, > 30 wt% PHA content was achieved using acetate, butyrate, propionate, lactate, and ethanol, with the highest content (60.3 wt%) using acetate. These results indicate that acetate-fed MMC by the ADD process could efficiently produce PHA from volatile fatty acids, lactate, pyruvate, and ethanol. Polyhydroxybutyrate was preferentially produced from acetate, butyrate, pyruvate, lactate, and ethanol, whereas polyhydroxyvalerate was notably produced from propionate. The results suggest that PHA can be efficiently produced from a wide range of substrates using MMCs enriched on a single substrate.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	97121
citation_title	Potential of activated sludge-derived mixed microbial culture enriched on acetate to produce polyhydroxyalkanoates from various substrates
citation_author	Ren, Yu
	Inoue, Daisuke
	Ike, Michihiko
citation_publisher	Springer
citation_language	英語
citation_date	2024-01-01
citation_journal_title	Journal of Material Cycles and Waste Management
citation_volume	26
citation_firstpage	2355
citation_lastpage	2365
citation_doi	https://doi.org/10.1007/s10163-024-01974-y
citation_public_url	https://hdl.handle.net/11094/97121

Potential of activated sludge-derived mixed microbial culture enriched on acetate to produce polyhydroxyalkanoates from various substrates

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