Phototuning of Hyaluronic-Acid-Based Hydrogel Properties to Control Network Formation in Human Vascular Endothelial Cells

Elvitigala, Kelum Chamara Manoj Lakmal; Mohan, Lakshmi; Mubarok, Wildan et al.

Advanced Healthcare Materials, 2024, 13(17), 2303787

アクセス数:365件（2025-07-03 18:07 集計）

固定URL: https://hdl.handle.net/11094/97143

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タイトル
Phototuning of Hyaluronic-Acid-Based Hydrogel Properties to Control Network Formation in Human Vascular Endothelial Cells
著者
著者名
Elvitigala, Kelum Chamara Manoj Lakmal
著者名
Mohan, Lakshmi
著者名
Mubarok, Wildan
NRID
1000020359938 1000020359938
ORCID
0000-0002-1041-4798 0000-0002-1041-4798
researchmap permalink
sakaishinji sakaishinji
Scopus Author ID
35450082900 35450082900
著者名
Sakai, Shinji
キーワード等
composite hydrogel
degradation
HUVEC
hyaluronic acid
photo-crosslinking
内容
Elvitigala K.C.M.L., Mohan L., Mubarok W., et al. Phototuning of Hyaluronic-Acid-Based Hydrogel Properties to Control Network Formation in Human Vascular Endothelial Cells. Advanced Healthcare Materials , (2024); https://doi.org/10.1002/adhm.202303787.
抄録
In vitro network formation by endothelial cells serves as a fundamental model for studies aimed at understanding angiogenesis. The morphogenesis of these cells to form a network is intricately regulated by the mechanical and biochemical properties of the extracellular matrix. Here the effects of modulating these properties in hydrogels derived from phenolated hyaluronic acid (HA-Ph) and phenolated gelatin (Gelatin-Ph) are presented. Visible-light irradiation in the presence of tris(2,2′-bipyridyl)ruthenium(II) chloride hexahydrate and sodium persulfate induces the crosslinking of these polymers, thereby forming a hydrogel and degrading HA-Ph. Human vascular endothelial cells form networks on the hydrogel prepared by visible-light irradiation for 45 min (42 W cm−2 at 450 nm) but not on the hydrogels prepared by irradiation for 15, 30, or 60 min. The irradiation time-dependent degradation of HA-Ph and the changes in the mechanical stiffness of the hydrogels, coupled with the expressions of RhoA and β-actin genes and CD44 receptors in the cells, reveal that the network formation is synergistically influenced by the hydrogel stiffness and HA-Ph degradation. These findings highlight the potential of tailoring HA-based hydrogel properties to modulate human vascular endothelial cell responses, which is critical for advancing their application in vascular tissue engineering.
出版者
John Wiley and Sons Inc
収録物名
Advanced Healthcare Materials
巻(号)
13(17)
ページ
2303787
刊行年月
2024-07-01
言語
英語
ハンドルURL
https://hdl.handle.net/11094/97143
PISSN
21922640
eISSN
21922659
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1002/adhm.202303787
アクセス権
open access
権利情報
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article