Swallowing-like activity elicited in neonatal rat medullary slice preparation

Kondo, Takahide; Yamanishi, Tadashi; Nishio, Takahiro; Yokota, Yusuke; Seikai, Tetsuya; Enomoto, Akifumi; Harada, Takeshi; Tsuji, Tadataka; Tanaka, Susumu

doi:https://doi.org/10.1016/j.brainres.2024.148955

Swallowing-like activity elicited in neonatal rat medullary slice preparation

Kondo, Takahide; Yamanishi, Tadashi; Nishio, Takahiro et al.

Brain Research, 2024, 1837, 148955

アクセス数:999件（2025-07-06 10:13 集計）

固定URL: https://hdl.handle.net/11094/97161

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タイトル	Swallowing-like activity elicited in neonatal rat medullary slice preparation
著者	NRID 1000090870444 1000090870444 著者名 Kondo, Takahide
	NRID 1000020397780 1000020397780 著者名 Yamanishi, Tadashi
	NRID 1000080974253 1000080974253 著者名 Nishio, Takahiro
	NRID 1000010448128 1000010448128 ORCID 0000-0001-9282-6544 0000-0001-9282-6544 researchmap permalink yusuke-yokota-0823 yusuke-yokota-0823 Scopus Author ID 56730005700 56730005700 著者名 Yokota, Yusuke
	NRID 1000040713194 1000040713194 著者名 Seikai, Tetsuya
	NRID 1000070432549 1000070432549 著者名 Enomoto, Akifumi
	NRID 1000000403030 1000000403030 researchmap permalink takeshi_harada takeshi_harada ORCID 0000-0002-6437-8614 0000-0002-6437-8614 Scopus Author ID 57205905423 57205905423 著者名 Harada, Takeshi
	NRID 1000050527231 1000050527231 著者名 Tsuji, Tadataka
	NRID 1000000367541 1000000367541 ORCID 0000-0002-0306-3627 0000-0002-0306-3627 researchmap permalink mes5 mes5 Scopus Author ID 56395750500 56395750500 著者名 Tanaka, Susumu
キーワード等	Central pattern generators
	Deglutition
	Electric stimulation
	Hypoglossal nerve
	Solitary nucleus
	Vagus nerve
内容	Kondo T., Yamanishi T., Nishio T., et al. Swallowing-like activity elicited in neonatal rat medullary slice preparation. Brain Research 1837, 148955 (2024); https://doi.org/10.1016/j.brainres.2024.148955.
抄録	Swallowing is induced by a central pattern generator in the nucleus tractus solitarius (NTS). We aimed to create a medullary slice preparation to elucidate the neural architecture of the central pattern generator of swallowing (Sw-CPG) and record its neural activities. Experiments were conducted on 2-day-old Sprague–Dawley rats (n = 46). The brainstem-spinal cord was transected at the pontomedullary and cervicothoracic junctions; the medulla was sliced transversely at thicknesses of 600, 700, or 800 μm. The rostral end of the slice was 100 μm rostral to the vagus nerve. We recorded hypoglossal nerve activity and electrically stimulated the vagus nerve or microinjected bicuculline methiodide (BIC) into the NTS. The 800-μm slices generated both rhythmic respiratory activity and electrically elicited neural activity. The 700-μm slices generated only respiratory activity, while the 600-μm slices did not generate any neural activity. BIC microinjection into the NTS in 800-μm slices resulted in the typical activity that closely resembled the swallowing activity reported in other experiments. This swallowing-like activity consistently lengthened the respiratory interval. Despite complete inhibition of respiratory activity, weak swallowing-like activity was observed under bath application of a non-NMDA receptor antagonist. Contrastingly, bath application of NMDA receptor antagonists resulted in a complete loss of swallowing-like activity and no change in respiratory activity. These results suggest that the 800-μm medullary slice preparation contains both afferent and efferent neural circuits and pattern generators of swallowing activity. Additionally, NMDA receptors may be necessary for generating swallowing activity. This medullary slice preparation can therefore elucidate Sw-CPG neural networks.
出版者	Elsevier B.V.
収録物名	Brain Research
巻(号)	1837
ページ	148955
刊行年月	2024-08-15
言語	英語
ハンドルURL	https://hdl.handle.net/11094/97161
PISSN	00068993
eISSN	18726240
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.brainres.2024.148955
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Brain Research. 2024, 1837, p. 148955
DC.title	Swallowing-like activity elicited in neonatal rat medullary slice preparation
DC.creator	Kondo, Takahide
	Yamanishi, Tadashi
	Nishio, Takahiro
	Yokota, Yusuke
	Seikai, Tetsuya
	Enomoto, Akifumi
	Harada, Takeshi
	Tsuji, Tadataka
	Tanaka, Susumu
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-08-15
DC.identifier	https://doi.org/10.1016/j.brainres.2024.148955
DC.subject	Central pattern generators
	Deglutition
	Electric stimulation
	Hypoglossal nerve
	Solitary nucleus
	Vagus nerve
DC.description	Kondo T., Yamanishi T., Nishio T., et al. Swallowing-like activity elicited in neonatal rat medullary slice preparation. Brain Research 1837, 148955 (2024); https://doi.org/10.1016/j.brainres.2024.148955.
DCTERMS.abstract	Swallowing is induced by a central pattern generator in the nucleus tractus solitarius (NTS). We aimed to create a medullary slice preparation to elucidate the neural architecture of the central pattern generator of swallowing (Sw-CPG) and record its neural activities. Experiments were conducted on 2-day-old Sprague–Dawley rats (n = 46). The brainstem-spinal cord was transected at the pontomedullary and cervicothoracic junctions; the medulla was sliced transversely at thicknesses of 600, 700, or 800 μm. The rostral end of the slice was 100 μm rostral to the vagus nerve. We recorded hypoglossal nerve activity and electrically stimulated the vagus nerve or microinjected bicuculline methiodide (BIC) into the NTS. The 800-μm slices generated both rhythmic respiratory activity and electrically elicited neural activity. The 700-μm slices generated only respiratory activity, while the 600-μm slices did not generate any neural activity. BIC microinjection into the NTS in 800-μm slices resulted in the typical activity that closely resembled the swallowing activity reported in other experiments. This swallowing-like activity consistently lengthened the respiratory interval. Despite complete inhibition of respiratory activity, weak swallowing-like activity was observed under bath application of a non-NMDA receptor antagonist. Contrastingly, bath application of NMDA receptor antagonists resulted in a complete loss of swallowing-like activity and no change in respiratory activity. These results suggest that the 800-μm medullary slice preparation contains both afferent and efferent neural circuits and pattern generators of swallowing activity. Additionally, NMDA receptors may be necessary for generating swallowing activity. This medullary slice preparation can therefore elucidate Sw-CPG neural networks.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	97161
citation_title	Swallowing-like activity elicited in neonatal rat medullary slice preparation
citation_author	Kondo, Takahide
	Yamanishi, Tadashi
	Nishio, Takahiro
	Yokota, Yusuke
	Seikai, Tetsuya
	Enomoto, Akifumi
	Harada, Takeshi
	Tsuji, Tadataka
	Tanaka, Susumu
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2024-08-15
citation_journal_title	Brain Research
citation_volume	1837
citation_firstpage	148955
citation_doi	https://doi.org/10.1016/j.brainres.2024.148955
citation_public_url	https://hdl.handle.net/11094/97161

Swallowing-like activity elicited in neonatal rat medullary slice preparation

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