Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis

Mwaniki, Stephen; Sawant, Priyanka; Osemwenkhae, Osaretin P.; Fujita, Yurika; Ito, Masaru; Furukohri, Asako; Shinohara, Akira

doi:https://doi.org/10.1111/gtc.13138

Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis

Mwaniki, Stephen; Sawant, Priyanka; Osemwenkhae, Osaretin P. et al.

Genes to Cells, 2024, 29(8), 650-666

アクセス数:573件（2025-07-03 22:53 集計）

固定URL: https://hdl.handle.net/11094/97194

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タイトル	Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis
著者	著者名 Mwaniki, Stephen
	著者名 Sawant, Priyanka
	著者名 Osemwenkhae, Osaretin P.
	NRID 1000050893913 1000050893913 ORCID 0000-0002-4350-7229 0000-0002-4350-7229 researchmap permalink yurikafujita yurikafujita Scopus Author ID 57146062800 57146062800 著者名 Fujita, Yurika
	NRID 1000030869061 1000030869061 researchmap permalink msrito msrito Scopus Author ID 35278000500 35278000500 著者名 Ito, Masaru
	NRID 1000090546293 1000090546293 researchmap permalink afurukohri afurukohri Scopus Author ID 25228605000 25228605000 著者名 Furukohri, Asako
	NRID 1000000252578 1000000252578 ORCID 0000-0003-4207-8247 0000-0003-4207-8247 researchmap permalink akirashinohara13 akirashinohara13 Scopus Author ID 7102342573 7102342573 著者名 Shinohara, Akira
キーワード等	cleavage
	Dmc1
	Mei5-Sae3
	meiotic recombination
	Rad51
内容	This is the peer reviewed version of the following article: Mwaniki S., Sawant P., Osemwenkhae O.P., et al. Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis. Genes to Cells, (2024) which has been published in final form at https://doi.org/10.1111/gtc.13138. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
抄録	Interhomolog recombination in meiosis is mediated by the Dmc1 recombinase. The Mei5-Sae3 complex of Saccharomyces cerevisiae promotes Dmc1 assembly and functions with Dmc1 for homology-mediated repair of meiotic DNA double-strand breaks. How Mei5-Sae3 facilitates Dmc1 assembly remains poorly understood. In this study, we created and characterized several mei5 mutants featuring the amino acid substitutions of basic residues. We found that Arg97 of Mei5, conserved in its ortholog, SFR1 (complex with SWI5), RAD51 mediator, in humans and other organisms, is critical for complex formation with Sae3 for Dmc1 assembly. Moreover, the substitution of either Arg117 or Lys133 with Ala in Mei5 resulted in the production of a C-terminal truncated Mei5 protein during yeast meiosis. Notably, the shorter Mei5-R117A protein was observed in meiotic cells but not in mitotic cells when expressed, suggesting a unique regulation of Dmc1-mediated recombination by posttranslational processing of Mei5-Sae3.
出版者	John Wiley and Sons Inc
収録物名	Genes to Cells
巻(号)	29(8)
ページ	650-666
刊行年月	2024-06-25
言語	英語
ハンドルURL	https://hdl.handle.net/11094/97194
PISSN	13569597
eISSN	13652443
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.1111/gtc.13138
アクセス権	embargoed access
権利情報	© 2024 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
権利情報	The full-text file will be made open to the public on 25 June 2025 in accordance with the publisher's policy.
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Genes to Cells. 2024, 29(8), p. 650-666
DC.title	Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis
DC.creator	Mwaniki, Stephen
	Sawant, Priyanka
	Osemwenkhae, Osaretin P.
	Fujita, Yurika
	Ito, Masaru
	Furukohri, Asako
	Shinohara, Akira
DC.publisher	John Wiley and Sons Inc
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-06-25
DC.identifier	https://doi.org/10.1111/gtc.13138
DC.subject	cleavage
	Dmc1
	Mei5-Sae3
	meiotic recombination
	Rad51
DC.description	This is the peer reviewed version of the following article: Mwaniki S., Sawant P., Osemwenkhae O.P., et al. Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis. Genes to Cells, (2024) which has been published in final form at https://doi.org/10.1111/gtc.13138. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
DCTERMS.abstract	Interhomolog recombination in meiosis is mediated by the Dmc1 recombinase. The Mei5-Sae3 complex of Saccharomyces cerevisiae promotes Dmc1 assembly and functions with Dmc1 for homology-mediated repair of meiotic DNA double-strand breaks. How Mei5-Sae3 facilitates Dmc1 assembly remains poorly understood. In this study, we created and characterized several mei5 mutants featuring the amino acid substitutions of basic residues. We found that Arg97 of Mei5, conserved in its ortholog, SFR1 (complex with SWI5), RAD51 mediator, in humans and other organisms, is critical for complex formation with Sae3 for Dmc1 assembly. Moreover, the substitution of either Arg117 or Lys133 with Ala in Mei5 resulted in the production of a C-terminal truncated Mei5 protein during yeast meiosis. Notably, the shorter Mei5-R117A protein was observed in meiotic cells but not in mitotic cells when expressed, suggesting a unique regulation of Dmc1-mediated recombination by posttranslational processing of Mei5-Sae3.
DC.rights	© 2024 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
DC.rights	The full-text file will be made open to the public on 25 June 2025 in accordance with the publisher's policy.
DC.format	97194
citation_title	Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis
citation_author	Mwaniki, Stephen
	Sawant, Priyanka
	Osemwenkhae, Osaretin P.
	Fujita, Yurika
	Ito, Masaru
	Furukohri, Asako
	Shinohara, Akira
citation_publisher	John Wiley and Sons Inc
citation_language	英語
citation_date	2024-06-25
citation_journal_title	Genes to Cells
citation_volume	29
citation_issue	8
citation_firstpage	650
citation_lastpage	666
citation_doi	https://doi.org/10.1111/gtc.13138
citation_public_url	https://hdl.handle.net/11094/97194

Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis

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