Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis

Sampathkumar, Arivarasan; Zhong, Chen; Tang, Yuting; Fujita, Yurika; Ito, Masaru; Shinohara, Akira

doi:https://doi.org/10.1038/s41598-024-60082-x

Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis

Sampathkumar, Arivarasan; Zhong, Chen; Tang, Yuting et al.

Scientific Reports, 2024, 14, 9550

アクセス数:54件（2025-05-18 10:25 集計）

固定URL: https://hdl.handle.net/11094/97217

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タイトル	Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis
著者	著者名 Sampathkumar, Arivarasan
	著者名 Zhong, Chen
	著者名 Tang, Yuting
	NRID 1000050893913 1000050893913 ORCID 0000-0002-4350-7229 0000-0002-4350-7229 researchmap permalink yurikafujita yurikafujita Scopus Author ID 57146062800 57146062800 著者名 Fujita, Yurika
	NRID 1000030869061 1000030869061 researchmap permalink msrito msrito Scopus Author ID 35278000500 35278000500 著者名 Ito, Masaru
	NRID 1000000252578 1000000252578 ORCID 0000-0003-4207-8247 0000-0003-4207-8247 researchmap permalink akirashinohara13 akirashinohara13 Scopus Author ID 7102342573 7102342573 著者名 Shinohara, Akira
キーワード等	Auxin degron
	Homologous recombination
	Meiosis
	Recombination checkpoint
	Rfa1
	RPA
	Synapsis checkpoint
内容	Sampathkumar A., Zhong C., Tang Y., et al. Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis. Scientific Reports 14, 9550 (2024); https://doi.org/10.1038/s41598-024-60082-x.
抄録	DNA double-strand breaks (DSBs) activate DNA damage responses (DDRs) in both mitotic and meiotic cells. A single-stranded DNA (ssDNA) binding protein, Replication protein-A (RPA) binds to the ssDNA formed at DSBs to activate ATR/Mec1 kinase for the response. Meiotic DSBs induce homologous recombination monitored by a meiotic DDR called the recombination checkpoint that blocks the pachytene exit in meiotic prophase I. In this study, we further characterized the essential role of RPA in the maintenance of the recombination checkpoint during Saccharomyces cerevisiae meiosis. The depletion of an RPA subunit, Rfa1, in a recombination-defective dmc1 mutant, fully alleviates the pachytene arrest with the persistent unrepaired DSBs. RPA depletion decreases the activity of a meiosis-specific CHK2 homolog, Mek1 kinase, which in turn activates the Ndt80 transcriptional regulator for pachytene exit. These support the idea that RPA is a sensor of ssDNAs for the activation of meiotic DDR. Rfa1 depletion also accelerates the prophase I delay in the zip1 mutant defective in both chromosome synapsis and the recombination, consistent with the notion that the accumulation of ssDNAs rather than defective synapsis triggers prophase I delay in the zip1 mutant.
出版者	Nature Research
収録物名	Scientific Reports
巻(号)	14
ページ	9550
刊行年月	2024-04-25
言語	英語
ハンドルURL	https://hdl.handle.net/11094/97217
eISSN	20452322
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41598-024-60082-x
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Scientific Reports. 2024, 14, p. 9550
DC.title	Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis
DC.creator	Sampathkumar, Arivarasan
	Zhong, Chen
	Tang, Yuting
	Fujita, Yurika
	Ito, Masaru
	Shinohara, Akira
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-04-25
DC.identifier	https://doi.org/10.1038/s41598-024-60082-x
DC.subject	Auxin degron
	Homologous recombination
	Meiosis
	Recombination checkpoint
	Rfa1
	RPA
	Synapsis checkpoint
DC.description	Sampathkumar A., Zhong C., Tang Y., et al. Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis. Scientific Reports 14, 9550 (2024); https://doi.org/10.1038/s41598-024-60082-x.
DCTERMS.abstract	DNA double-strand breaks (DSBs) activate DNA damage responses (DDRs) in both mitotic and meiotic cells. A single-stranded DNA (ssDNA) binding protein, Replication protein-A (RPA) binds to the ssDNA formed at DSBs to activate ATR/Mec1 kinase for the response. Meiotic DSBs induce homologous recombination monitored by a meiotic DDR called the recombination checkpoint that blocks the pachytene exit in meiotic prophase I. In this study, we further characterized the essential role of RPA in the maintenance of the recombination checkpoint during Saccharomyces cerevisiae meiosis. The depletion of an RPA subunit, Rfa1, in a recombination-defective dmc1 mutant, fully alleviates the pachytene arrest with the persistent unrepaired DSBs. RPA depletion decreases the activity of a meiosis-specific CHK2 homolog, Mek1 kinase, which in turn activates the Ndt80 transcriptional regulator for pachytene exit. These support the idea that RPA is a sensor of ssDNAs for the activation of meiotic DDR. Rfa1 depletion also accelerates the prophase I delay in the zip1 mutant defective in both chromosome synapsis and the recombination, consistent with the notion that the accumulation of ssDNAs rather than defective synapsis triggers prophase I delay in the zip1 mutant.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	97217
citation_title	Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis
citation_author	Sampathkumar, Arivarasan
	Zhong, Chen
	Tang, Yuting
	Fujita, Yurika
	Ito, Masaru
	Shinohara, Akira
citation_publisher	Nature Research
citation_language	英語
citation_date	2024-04-25
citation_journal_title	Scientific Reports
citation_volume	14
citation_firstpage	9550
citation_doi	https://doi.org/10.1038/s41598-024-60082-x
citation_public_url	https://hdl.handle.net/11094/97217

Replication protein-A, RPA, plays a pivotal role in the maintenance of recombination checkpoint in yeast meiosis

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