Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity

Ogaya, Yuko; Kadota, Tamami; Hamada, Masakazu; Nomura, Ryota; Nakano, Kazuhiko

doi:https://doi.org/10.1080/20002297.2024.2339158

Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity

Ogaya, Yuko; Kadota, Tamami; Hamada, Masakazu et al.

Journal of Oral Microbiology, 2024, 16(1), 2339158

アクセス数:37件（2025-04-10 22:53 集計）

固定URL: https://hdl.handle.net/11094/97219

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タイトル	Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity
著者	NRID 1000040803078 1000040803078 researchmap permalink y_ogaya y_ogaya Scopus Author ID 56460237100 56460237100 著者名 Ogaya, Yuko
	NRID 1000010908643 1000010908643 researchmap permalink kadota-tamami kadota-tamami 著者名 Kadota, Tamami
	NRID 1000080506361 1000080506361 researchmap permalink 6361 6361 Scopus Author ID 36616148000 36616148000 著者名 Hamada, Masakazu
	NRID 1000090437385 1000090437385 著者名 Nomura, Ryota
	NRID 1000000379083 1000000379083 researchmap permalink read0202427 read0202427 Scopus Author ID 7402011667 7402011667 著者名 Nakano, Kazuhiko
キーワード等	Helicobacter pylori
	metagenomic analysis
	oral cavity
	oral microbiome
	saliva
内容	Ogaya Y., Kadota T., Hamada M., et al. Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity. Journal of Oral Microbiology 16, 2339158 (2024); https://doi.org/10.1080/20002297.2024.2339158.
抄録	Objective: Helicobacter pylori infection is acquired in childhood via the oral cavity, although its relationship with the characteristics of the oral microbiome has not been elucidated. In this study, we performed comprehensive analysis of the oral microbiome in children and adults with or without H. pylori in the oral cavity. Methods: Bacterial DNA was extracted from 41 adult and 21 child saliva specimens, and H. pylori was detected using PCR. 16S rRNA gene amplification was performed for next-generation sequencing. Bioinformatic analyses were conducted using Quantitative Insights into Microbial Ecology 2 (QIIME 2). Results: Faith’s phylogenetic diversity analysis showed a significant difference between H. pylori-negative adult and child specimens in terms of α-diversity (p < 0.05), while no significant difference was observed between H. pylori-positive adult and child specimens. There was also a significant difference in β-diversity between H. pylori-positive and negative child specimens (p < 0.05). Taxonomic analysis at the genus level revealed that Porphyromonas was the only bacterium that was significantly more abundant in both H. pylori-positive adults and children than in corresponding negative specimens (p < 0.01 and p < 0.05, respectively). Conclusion: These results suggest unique oral microbiome characteristics in children with H. pylori infection in the oral cavity.
出版者	Taylor and Francis Ltd.
収録物名	Journal of Oral Microbiology
巻(号)	16(1)
ページ	2339158
刊行年月	2024-04-11
言語	英語
ハンドルURL	https://hdl.handle.net/11094/97219
eISSN	20002297
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1080/20002297.2024.2339158
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Journal of Oral Microbiology. 2024, 16(1), p. 2339158
DC.title	Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity
DC.creator	Ogaya, Yuko
	Kadota, Tamami
	Hamada, Masakazu
	Nomura, Ryota
	Nakano, Kazuhiko
DC.publisher	Taylor and Francis Ltd.
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-04-11
DC.identifier	https://doi.org/10.1080/20002297.2024.2339158
DC.subject	Helicobacter pylori
	metagenomic analysis
	oral cavity
	oral microbiome
	saliva
DC.description	Ogaya Y., Kadota T., Hamada M., et al. Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity. Journal of Oral Microbiology 16, 2339158 (2024); https://doi.org/10.1080/20002297.2024.2339158.
DCTERMS.abstract	Objective: Helicobacter pylori infection is acquired in childhood via the oral cavity, although its relationship with the characteristics of the oral microbiome has not been elucidated. In this study, we performed comprehensive analysis of the oral microbiome in children and adults with or without H. pylori in the oral cavity. Methods: Bacterial DNA was extracted from 41 adult and 21 child saliva specimens, and H. pylori was detected using PCR. 16S rRNA gene amplification was performed for next-generation sequencing. Bioinformatic analyses were conducted using Quantitative Insights into Microbial Ecology 2 (QIIME 2). Results: Faith’s phylogenetic diversity analysis showed a significant difference between H. pylori-negative adult and child specimens in terms of α-diversity (p < 0.05), while no significant difference was observed between H. pylori-positive adult and child specimens. There was also a significant difference in β-diversity between H. pylori-positive and negative child specimens (p < 0.05). Taxonomic analysis at the genus level revealed that Porphyromonas was the only bacterium that was significantly more abundant in both H. pylori-positive adults and children than in corresponding negative specimens (p < 0.01 and p < 0.05, respectively). Conclusion: These results suggest unique oral microbiome characteristics in children with H. pylori infection in the oral cavity.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	97219
citation_title	Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity
citation_author	Ogaya, Yuko
	Kadota, Tamami
	Hamada, Masakazu
	Nomura, Ryota
	Nakano, Kazuhiko
citation_publisher	Taylor and Francis Ltd.
citation_language	英語
citation_date	2024-04-11
citation_journal_title	Journal of Oral Microbiology
citation_volume	16
citation_issue	1
citation_firstpage	2339158
citation_doi	https://doi.org/10.1080/20002297.2024.2339158
citation_public_url	https://hdl.handle.net/11094/97219

Characterization of the unique oral microbiome of children harboring Helicobacter pylori in the oral cavity

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