Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging

Rui, Xuehui; Okamoto, Yukihiro; Fukushima, Shuichiro; Morishita Watanabe, Nozomi; Umakoshi, Hiroshi

doi:https://doi.org/10.1038/s41598-024-65812-9

Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging

Rui, Xuehui; Okamoto, Yukihiro; Fukushima, Shuichiro et al.

Scientific Reports, 2024, 14(1), 15831

アクセス数:764件（2025-07-10 23:48 集計）

固定URL: https://hdl.handle.net/11094/97844

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タイトル	Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging
著者	著者名 Rui, Xuehui
	NRID 1000050503918 1000050503918 researchmap permalink read1-okamoto read1-okamoto Scopus Author ID 35269706600 35269706600 著者名 Okamoto, Yukihiro
	NRID 1000040362644 1000040362644 researchmap permalink read0107422 read0107422 Scopus Author ID 7202543214 7202543214 著者名 Fukushima, Shuichiro
	NRID 1000040892683 1000040892683 ORCID 0000-0002-3461-3660 0000-0002-3461-3660 researchmap permalink nozomiwatanabe nozomiwatanabe Scopus Author ID 57190045741 57190045741 著者名 Morishita Watanabe, Nozomi
	NRID 1000020311772 1000020311772 researchmap permalink read0191167 read0191167 Scopus Author ID 6701925853 6701925853 著者名 Umakoshi, Hiroshi
内容	Rui X., Okamoto Y., Fukushima S., et al. Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging. Scientific Reports 14, 15831 (2024); https://doi.org/10.1038/s41598-024-65812-9.
抄録	2-Hydroxyoleic acid (2-OHOA) has gained attention as a membrane lipid therapy (MLT) anti-cancer drug. However, in the viewpoint of anti-cancer drug, 2-OHOA shows poor water solubility and its effectiveness still has space for improvement. Thus, this study aimed to overcome the problems by formulating 2-OHOA into liposome dosage form. Furthermore, in the context of MLT reagents, the influence of 2-OHOA on the biophysical properties of the cytoplasmic membrane remains largely unexplored. To bridge this gap, our study specifically focused the alterations in cancer cell membrane fluidity and lipid packing characteristics before and after treatment. By using a two-photon microscope and the Laurdan fluorescence probe, we noted that liposomes incorporating 2-OHOA induced a more significant reduction in cancer cell membrane fluidity, accompanied by a heightened rate of cellular apoptosis when compared to the non-formulated 2-OHOA. Importantly, the enhanced efficacy of 2-OHOA within the liposomal formulation demonstrated a correlation with its endocytic uptake mechanism. In conclusion, our findings underscore the significant influence of 2-OHOA on the biophysical properties of cancer plasma membranes, emphasizing the potential of liposomes as an optimized delivery system for 2-OHOA in anti-cancer therapy.
出版者	Nature Research
収録物名	Scientific Reports
巻(号)	14(1)
ページ	15831
刊行年月	2024-07-09
言語	英語
ハンドルURL	https://hdl.handle.net/11094/97844
eISSN	20452322
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41598-024-65812-9
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Scientific Reports. 2024, 14(1), p. 15831
DC.title	Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging
DC.creator	Rui, Xuehui
	Okamoto, Yukihiro
	Fukushima, Shuichiro
	Morishita Watanabe, Nozomi
	Umakoshi, Hiroshi
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-07-09
DC.identifier	https://doi.org/10.1038/s41598-024-65812-9
DC.description	Rui X., Okamoto Y., Fukushima S., et al. Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging. Scientific Reports 14, 15831 (2024); https://doi.org/10.1038/s41598-024-65812-9.
DCTERMS.abstract	2-Hydroxyoleic acid (2-OHOA) has gained attention as a membrane lipid therapy (MLT) anti-cancer drug. However, in the viewpoint of anti-cancer drug, 2-OHOA shows poor water solubility and its effectiveness still has space for improvement. Thus, this study aimed to overcome the problems by formulating 2-OHOA into liposome dosage form. Furthermore, in the context of MLT reagents, the influence of 2-OHOA on the biophysical properties of the cytoplasmic membrane remains largely unexplored. To bridge this gap, our study specifically focused the alterations in cancer cell membrane fluidity and lipid packing characteristics before and after treatment. By using a two-photon microscope and the Laurdan fluorescence probe, we noted that liposomes incorporating 2-OHOA induced a more significant reduction in cancer cell membrane fluidity, accompanied by a heightened rate of cellular apoptosis when compared to the non-formulated 2-OHOA. Importantly, the enhanced efficacy of 2-OHOA within the liposomal formulation demonstrated a correlation with its endocytic uptake mechanism. In conclusion, our findings underscore the significant influence of 2-OHOA on the biophysical properties of cancer plasma membranes, emphasizing the potential of liposomes as an optimized delivery system for 2-OHOA in anti-cancer therapy.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	97844
citation_title	Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging
citation_author	Rui, Xuehui
	Okamoto, Yukihiro
	Fukushima, Shuichiro
	Morishita Watanabe, Nozomi
	Umakoshi, Hiroshi
citation_publisher	Nature Research
citation_language	英語
citation_date	2024-07-09
citation_journal_title	Scientific Reports
citation_volume	14
citation_issue	1
citation_firstpage	15831
citation_doi	https://doi.org/10.1038/s41598-024-65812-9
citation_public_url	https://hdl.handle.net/11094/97844

Investigating the impact of 2-OHOA-embedded liposomes on biophysical properties of cancer cell membranes via Laurdan two-photon microscopy imaging

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