Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues

Watanabe, Kenichi; Takayama, Sayo; Yamada, Toichiro; Hashimoto, Masayo; Tadano, Jun; Nakagawa, Tetsuya; Watanabe, Takao; Fukusaki, Eiichiro; Miyawaki, Izuru; Shimma, Shuichi

doi:https://doi.org/10.1007/s00216-024-05477-5

Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues

Watanabe, Kenichi; Takayama, Sayo; Yamada, Toichiro et al.

Analytical and Bioanalytical Chemistry, 2024, 416(26), 5579-5593

アクセス数:197件（2025-06-07 19:18 集計）

固定URL: https://hdl.handle.net/11094/98111

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タイトル	Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues
著者	著者名 Watanabe, Kenichi
	著者名 Takayama, Sayo
	著者名 Yamada, Toichiro
	著者名 Hashimoto, Masayo
	著者名 Tadano, Jun
	著者名 Nakagawa, Tetsuya
	著者名 Watanabe, Takao
	NRID 1000040273594 1000040273594 ORCID 0000-0003-3069-5547 0000-0003-3069-5547 researchmap permalink fukusaki-eiichiro fukusaki-eiichiro Scopus Author ID 35473325000 35473325000 著者名 Fukusaki, Eiichiro
	著者名 Miyawaki, Izuru
	NRID 1000030515896 1000030515896 researchmap permalink shimmax shimmax Scopus Author ID 23486936900 23486936900 著者名 Shimma, Shuichi
キーワード等	Brain
	Clozapine
	Matrix-assisted laser desorption/ionization
	Neurotransmitters
	Quantitative mass spectrometry imaging
内容	The version of record of this article, first published in Analytical and Bioanalytical Chemistry, is available online at Publisher’s website: https://doi.org/10.1007/s00216-024-05477-5.
抄録	Understanding the relationship between the concentration of a drug and its therapeutic efficacy or side effects is crucial in drug development, especially to understand therapeutic efficacy in central nervous system drug, quantifying drug-induced site-specific changes in the levels of endogenous metabolites, such as neurotransmitters. In recent times, evaluation of quantitative distribution of drugs and endogenous metabolites using matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) has attracted much attention in drug discovery research. However, MALDI-MSI quantification (quantitative mass spectrometry imaging, QMSI) is an emerging technique, and needs to be further developed for practicable and convenient use in drug discovery research. In this study, we developed a reliable QMSI method for quantification of clozapine (antipsychotic drug) and dopamine and its metabolites in the rat brain using MALDI-MSI. An improved mimetic tissue model using powdered frozen tissue for QMSI was established as an alternative method, enabling the accurate quantification of clozapine levels in the rat brain. Furthermore, we used the improved method to evaluate drug-induced fluctuations in the concentrations of dopamine and its metabolites. This method can quantitatively evaluate drug localization in the brain and drug-induced changes in the concentration of endogenous metabolites, demonstrating the usefulness of QMSI.
出版者	Springer Science and Business Media Deutschland GmbH
収録物名	Analytical and Bioanalytical Chemistry
巻(号)	416(26)
ページ	5579-5593
刊行年月	2024-08-10
言語	英語
ハンドルURL	https://hdl.handle.net/11094/98111
PISSN	16182642
eISSN	16182650
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1007/s00216-024-05477-5
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

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資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Analytical and Bioanalytical Chemistry. 2024, 416(26), p. 5579-5593
DC.title	Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues
DC.creator	Watanabe, Kenichi
	Takayama, Sayo
	Yamada, Toichiro
	Hashimoto, Masayo
	Tadano, Jun
	Nakagawa, Tetsuya
	Watanabe, Takao
	Fukusaki, Eiichiro
	Miyawaki, Izuru
	Shimma, Shuichi
DC.publisher	Springer Science and Business Media Deutschland GmbH
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-08-10
DC.identifier	https://doi.org/10.1007/s00216-024-05477-5
DC.subject	Brain
	Clozapine
	Matrix-assisted laser desorption/ionization
	Neurotransmitters
	Quantitative mass spectrometry imaging
DC.description	The version of record of this article, first published in Analytical and Bioanalytical Chemistry, is available online at Publisher’s website: https://doi.org/10.1007/s00216-024-05477-5.
DCTERMS.abstract	Understanding the relationship between the concentration of a drug and its therapeutic efficacy or side effects is crucial in drug development, especially to understand therapeutic efficacy in central nervous system drug, quantifying drug-induced site-specific changes in the levels of endogenous metabolites, such as neurotransmitters. In recent times, evaluation of quantitative distribution of drugs and endogenous metabolites using matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) has attracted much attention in drug discovery research. However, MALDI-MSI quantification (quantitative mass spectrometry imaging, QMSI) is an emerging technique, and needs to be further developed for practicable and convenient use in drug discovery research. In this study, we developed a reliable QMSI method for quantification of clozapine (antipsychotic drug) and dopamine and its metabolites in the rat brain using MALDI-MSI. An improved mimetic tissue model using powdered frozen tissue for QMSI was established as an alternative method, enabling the accurate quantification of clozapine levels in the rat brain. Furthermore, we used the improved method to evaluate drug-induced fluctuations in the concentrations of dopamine and its metabolites. This method can quantitatively evaluate drug localization in the brain and drug-induced changes in the concentration of endogenous metabolites, demonstrating the usefulness of QMSI.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	98111
citation_title	Novel mimetic tissue standards for precise quantitative mass spectrometry imaging of drug and neurotransmitter concentrations in rat brain tissues
citation_author	Watanabe, Kenichi
	Takayama, Sayo
	Yamada, Toichiro
	Hashimoto, Masayo
	Tadano, Jun
	Nakagawa, Tetsuya
	Watanabe, Takao
	Fukusaki, Eiichiro
	Miyawaki, Izuru
	Shimma, Shuichi
citation_publisher	Springer Science and Business Media Deutschland GmbH
citation_language	英語
citation_date	2024-08-10
citation_journal_title	Analytical and Bioanalytical Chemistry
citation_volume	416
citation_issue	26
citation_firstpage	5579
citation_lastpage	5593
citation_doi	https://doi.org/10.1007/s00216-024-05477-5
citation_public_url	https://hdl.handle.net/11094/98111