MYCBPAP is a central apparatus protein required for centrosome–nuclear envelope docking and sperm tail biogenesis in mice

Wang, Haoting; Kobayashi, Hiroko; Shimada, Keisuke; Oura, Seiya; Oyama, Yuki; Kitakaze, Hiroaki; Noda, Taichi; Yabuta, Norikazu; Miyata, Haruhiko; Ikawa, Masahito

doi:https://doi.org/10.1242/jcs.261962

アクセス数:18件（2025-01-03 16:19 集計）

固定URL: https://hdl.handle.net/11094/98222

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タイトル	MYCBPAP is a central apparatus protein required for centrosome–nuclear envelope docking and sperm tail biogenesis in mice
著者	著者名 Wang, Haoting
	著者名 Kobayashi, Hiroko
	NRID 1000060779601 1000060779601 ORCID 0000-0003-3739-7163 0000-0003-3739-7163 researchmap permalink nununununu nununununu Scopus Author ID 56479808000 56479808000 著者名 Shimada, Keisuke
	著者名 Oura, Seiya
	ORCID 0000-0003-4579-5487 0000-0003-4579-5487 著者名 Oyama, Yuki
	NRID 1000050981958 1000050981958 researchmap permalink hiroakikitakaze hiroakikitakaze 著者名 Kitakaze, Hiroaki
	NRID 1000050712551 1000050712551 著者名 Noda, Taichi
	NRID 1000010343245 1000010343245 researchmap permalink read0092303 read0092303 Scopus Author ID 6603956412 6603956412 著者名 Yabuta, Norikazu
	NRID 1000050604732 1000050604732 ORCID 0000-0003-4758-5803 0000-0003-4758-5803 researchmap permalink hmiyata hmiyata Scopus Author ID 37161837000 37161837000 著者名 Miyata, Haruhiko
	NRID 1000020304066 1000020304066 ORCID 0000-0001-9859-6217 0000-0001-9859-6217 researchmap permalink read0054910 read0054910 Scopus Author ID 16689054500 16689054500 著者名 Ikawa, Masahito
キーワード等	Central pair
	Centriole
	Flagellum formation
	Genome editing
	Knockout mice
内容	Wang H., Kobayashi H., Shimada K., et al. MYCBPAP is a central apparatus protein required for centrosome–nuclear envelope docking and sperm tail biogenesis in mice. Journal of Cell Science 137, jcs261962 (2024); https://doi.org/10.1242/jcs.261962.
抄録	The structure of the sperm flagellar axoneme is highly conserved across species and serves the essential function of generating motility to facilitate the meeting of spermatozoa with the egg. During spermiogenesis, the axoneme elongates from the centrosome, and subsequently the centrosome docks onto the nuclear envelope to continue tail biogenesis. Mycbpap is expressed predominantly in mouse and human testes and conserved in Chlamydomonas as FAP147. A previous cryo-electron microscopy analysis has revealed the localization of FAP147 to the central apparatus of the axoneme. Here, we generated Mycbpap-knockout mice and demonstrated the essential role of Mycbpap in male fertility. Deletion of Mycbpap led to disrupted centrosome–nuclear envelope docking and abnormal flagellar biogenesis. Furthermore, we generated transgenic mice with tagged MYCBPAP, which restored the fertility of Mycbpap-knockout males. Interactome analyses of MYCBPAP using Mycbpap transgenic mice unveiled binding partners of MYCBPAP including central apparatus proteins, such as CFAP65 and CFAP70, which constitute the C2a projection, and centrosome-associated proteins, such as CCP110. These findings provide insights into a MYCBPAP-dependent regulation of the centrosome–nuclear envelope docking and sperm tail biogenesis.
出版者	Company of Biologists Ltd
収録物名	Journal of Cell Science
巻(号)	137(16)
ページ	jcs261962
刊行年月	2024-08-29
言語	英語
ハンドルURL	https://hdl.handle.net/11094/98222
PISSN	00219533
eISSN	14779137
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1242/jcs.261962
アクセス権	embargoed access
権利情報	The full-text file will be made open to the public on 29 August 2025 in accordance with the publisher's policy.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Journal of Cell Science. 2024, 137(16), p. jcs261962
DC.title	MYCBPAP is a central apparatus protein required for centrosome–nuclear envelope docking and sperm tail biogenesis in mice
DC.creator	Wang, Haoting
	Kobayashi, Hiroko
	Shimada, Keisuke
	Oura, Seiya
	Oyama, Yuki
	Kitakaze, Hiroaki
	Noda, Taichi
	Yabuta, Norikazu
	Miyata, Haruhiko
	Ikawa, Masahito
DC.publisher	Company of Biologists Ltd
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-08-29
DC.identifier	https://doi.org/10.1242/jcs.261962
DC.subject	Central pair
	Centriole
	Flagellum formation
	Genome editing
	Knockout mice
DC.description	Wang H., Kobayashi H., Shimada K., et al. MYCBPAP is a central apparatus protein required for centrosome–nuclear envelope docking and sperm tail biogenesis in mice. Journal of Cell Science 137, jcs261962 (2024); https://doi.org/10.1242/jcs.261962.
DCTERMS.abstract	The structure of the sperm flagellar axoneme is highly conserved across species and serves the essential function of generating motility to facilitate the meeting of spermatozoa with the egg. During spermiogenesis, the axoneme elongates from the centrosome, and subsequently the centrosome docks onto the nuclear envelope to continue tail biogenesis. Mycbpap is expressed predominantly in mouse and human testes and conserved in Chlamydomonas as FAP147. A previous cryo-electron microscopy analysis has revealed the localization of FAP147 to the central apparatus of the axoneme. Here, we generated Mycbpap-knockout mice and demonstrated the essential role of Mycbpap in male fertility. Deletion of Mycbpap led to disrupted centrosome–nuclear envelope docking and abnormal flagellar biogenesis. Furthermore, we generated transgenic mice with tagged MYCBPAP, which restored the fertility of Mycbpap-knockout males. Interactome analyses of MYCBPAP using Mycbpap transgenic mice unveiled binding partners of MYCBPAP including central apparatus proteins, such as CFAP65 and CFAP70, which constitute the C2a projection, and centrosome-associated proteins, such as CCP110. These findings provide insights into a MYCBPAP-dependent regulation of the centrosome–nuclear envelope docking and sperm tail biogenesis.
DC.rights	The full-text file will be made open to the public on 29 August 2025 in accordance with the publisher's policy.
DC.format	98222
citation_title	MYCBPAP is a central apparatus protein required for centrosome–nuclear envelope docking and sperm tail biogenesis in mice
citation_author	Wang, Haoting
	Kobayashi, Hiroko
	Shimada, Keisuke
	Oura, Seiya
	Oyama, Yuki
	Kitakaze, Hiroaki
	Noda, Taichi
	Yabuta, Norikazu
	Miyata, Haruhiko
	Ikawa, Masahito
citation_publisher	Company of Biologists Ltd
citation_language	英語
citation_date	2024-08-29
citation_journal_title	Journal of Cell Science
citation_volume	137
citation_issue	16
citation_firstpage	jcs261962
citation_doi	https://doi.org/10.1242/jcs.261962
citation_public_url	https://hdl.handle.net/11094/98222

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