Reviving Sternheimer stain: A single-center retrospective study to detect the diagnostic utility of urinary tract infections in the emergency department

Nagatomi, Hikaru; Sada, Ryuichi Minoda; Abe, Noriyuki; Miyake, Hirofumi; Akebo, Hiroyuki

doi:https://doi.org/10.1016/j.jiac.2024.02.019

Reviving Sternheimer stain: A single-center retrospective study to detect the diagnostic utility of urinary tract infections in the emergency department

Nagatomi, Hikaru; Sada, Ryuichi Minoda; Abe, Noriyuki et al.

Journal of Infection and Chemotherapy, 2024, 30(8), 768-772

アクセス数:324件（2025-07-04 23:24 集計）

固定URL: https://hdl.handle.net/11094/98225

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タイトル	Reviving Sternheimer stain: A single-center retrospective study to detect the diagnostic utility of urinary tract infections in the emergency department
著者	著者名 Nagatomi, Hikaru
	NRID 1000040961230 1000040961230 ORCID 0000-0002-1455-6822 0000-0002-1455-6822 researchmap permalink sadametal sadametal Scopus Author ID 59319135000 59319135000 著者名 Sada, Ryuichi Minoda
	著者名 Abe, Noriyuki
	著者名 Miyake, Hirofumi
	著者名 Akebo, Hiroyuki
キーワード等	Gram stain
	Pyuria
	Sternheimer stain
内容	Nagatomi H., Sada R.M., Abe N., et al. Reviving Sternheimer stain: A single-center retrospective study to detect the diagnostic utility of urinary tract infections in the emergency department. Journal of Infection and Chemotherapy 30, 768 (2024); https://doi.org/10.1016/j.jiac.2024.02.019.
抄録	Introduction: Qualitative urinalysis using the Sternheimer stain is a common method in Japan for identifying bacteriuria, but there is a lack of studies examining its test characteristics. In this study, we aimed to investigate the sensitivity and specificity of the Sternheimer stain for urine culture results and compare it with the sensitivity and specificity of the Gram stain. Our goal was to determine the usefulness of the Sternheimer stain in identifying bacteriuria. Patients and methods: Among 986 patients aged 16 years or older from whom samples for both urinalysis and urine culture were obtained at the emergency room of Tenri Hospital from January 2019 to December 2019, 342 patients with pyuria, defined as the presence of 10 or more white cells per cubic millimeter in a urine specimen, who had not received prior antimicrobial therapy were included. Urine cultures were used for comparison to determine the sensitivity and specificity of Sternheimer and Gram stain in this patient group. A positive Sternheimer stain result was defined as bacteriuria ≥ (1+), and that of Gram stain was defined as ≥ 1/1 field of high-power ( × 1000) oil immersion. Results: Using urine culture results for comparison, the sensitivity of Sternheimer stain was 92.2%, the specificity was 48.5%, the positive likelihood ratio was 1.79, and the negative likelihood ratio was 0.16. Discussion: Sternheimer stain is a rapid and useful method to exclude bacteriuria in a group of patients with pyuria in the emergency department.
出版者	Elsevier B.V.
収録物名	Journal of Infection and Chemotherapy
巻(号)	30(8)
ページ	768-772
刊行年月	2024-08-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/98225
PISSN	1341321X
eISSN	14377780
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.1016/j.jiac.2024.02.019
アクセス権	embargoed access
権利情報	© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
権利情報	The full-text file will be made open to the public on 1 August 2025 in accordance with the publisher's policy.
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Journal of Infection and Chemotherapy. 2024, 30(8), p. 768-772
DC.title	Reviving Sternheimer stain: A single-center retrospective study to detect the diagnostic utility of urinary tract infections in the emergency department
DC.creator	Nagatomi, Hikaru
	Sada, Ryuichi Minoda
	Abe, Noriyuki
	Miyake, Hirofumi
	Akebo, Hiroyuki
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-08-01
DC.identifier	https://doi.org/10.1016/j.jiac.2024.02.019
DC.subject	Gram stain
	Pyuria
	Sternheimer stain
DC.description	Nagatomi H., Sada R.M., Abe N., et al. Reviving Sternheimer stain: A single-center retrospective study to detect the diagnostic utility of urinary tract infections in the emergency department. Journal of Infection and Chemotherapy 30, 768 (2024); https://doi.org/10.1016/j.jiac.2024.02.019.
DCTERMS.abstract	Introduction: Qualitative urinalysis using the Sternheimer stain is a common method in Japan for identifying bacteriuria, but there is a lack of studies examining its test characteristics. In this study, we aimed to investigate the sensitivity and specificity of the Sternheimer stain for urine culture results and compare it with the sensitivity and specificity of the Gram stain. Our goal was to determine the usefulness of the Sternheimer stain in identifying bacteriuria. Patients and methods: Among 986 patients aged 16 years or older from whom samples for both urinalysis and urine culture were obtained at the emergency room of Tenri Hospital from January 2019 to December 2019, 342 patients with pyuria, defined as the presence of 10 or more white cells per cubic millimeter in a urine specimen, who had not received prior antimicrobial therapy were included. Urine cultures were used for comparison to determine the sensitivity and specificity of Sternheimer and Gram stain in this patient group. A positive Sternheimer stain result was defined as bacteriuria ≥ (1+), and that of Gram stain was defined as ≥ 1/1 field of high-power ( × 1000) oil immersion. Results: Using urine culture results for comparison, the sensitivity of Sternheimer stain was 92.2%, the specificity was 48.5%, the positive likelihood ratio was 1.79, and the negative likelihood ratio was 0.16. Discussion: Sternheimer stain is a rapid and useful method to exclude bacteriuria in a group of patients with pyuria in the emergency department.
DC.rights	© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
DC.rights	The full-text file will be made open to the public on 1 August 2025 in accordance with the publisher's policy.
DC.format	98225
citation_title	Reviving Sternheimer stain: A single-center retrospective study to detect the diagnostic utility of urinary tract infections in the emergency department
citation_author	Nagatomi, Hikaru
	Sada, Ryuichi Minoda
	Abe, Noriyuki
	Miyake, Hirofumi
	Akebo, Hiroyuki
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2024-08-01
citation_journal_title	Journal of Infection and Chemotherapy
citation_volume	30
citation_issue	8
citation_firstpage	768
citation_lastpage	772
citation_doi	https://doi.org/10.1016/j.jiac.2024.02.019
citation_public_url	https://hdl.handle.net/11094/98225

Reviving Sternheimer stain: A single-center retrospective study to detect the diagnostic utility of urinary tract infections in the emergency department

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