CheB localizes to polar receptor arrays during repellent adaptation

Fukuoka, Hajime; Nishitani, Keisuke; Deguchi, Taiga; Oshima, Taketo; Uchida, Yumiko; Hamamoto, Tatsuki; Che, Yong Suk; Ishijima, Akihiko

doi:https://doi.org/10.1126/sciadv.adp5636

CheB localizes to polar receptor arrays during repellent adaptation

Fukuoka, Hajime; Nishitani, Keisuke; Deguchi, Taiga et al.

Science Advances, 2024, 10(38), eadp5636

アクセス数:22件（2025-03-17 07:14 集計）

固定URL: https://hdl.handle.net/11094/98253

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タイトル	CheB localizes to polar receptor arrays during repellent adaptation
著者	NRID 1000050447190 1000050447190 ORCID 0000-0002-9060-4347 0000-0002-9060-4347 researchmap permalink fps_475-511-530-610 fps_475-511-530-610 Scopus Author ID 7103098825 7103098825 著者名 Fukuoka, Hajime
	著者名 Nishitani, Keisuke
	著者名 Deguchi, Taiga
	著者名 Oshima, Taketo
	researchmap permalink uchidayumiko uchidayumiko Scopus Author ID 55481850700 55481850700 著者名 Uchida, Yumiko
	著者名 Hamamoto, Tatsuki
	著者名 Che, Yong Suk
	NRID 1000080301216 1000080301216 ORCID 0000-0002-8919-9268 0000-0002-8919-9268 researchmap permalink A.Ishijima A.Ishijima Scopus Author ID 7004278926 7004278926 著者名 Ishijima, Akihiko
内容	Fukuoka H., Nishitani K., Deguchi T., et al. CheB localizes to polar receptor arrays during repellent adaptation. Science Advances 10, eadp5636 (2024); https://doi.org/10.1126/sciadv.adp5636.
抄録	Adaptation of the response to stimuli is a fundamental process for all organisms. Here, we show that the adaptation enzyme CheB methylesterase of Escherichia coli assembles to the ON state receptor array after exposure to the repellent l-isoleucine and dissociates from the array after adaptation is complete. The duration of increased CheB localization and the time of highly clockwise-biased flagellar rotation were similar and depended on the strength of the stimulus. The increase in CheB at the receptor array and the decrease in cytoplasmic CheB were both ~100 molecules, which represents 15 to 20% of the total cellular content of CheB. We confirmed that the main binding site for CheB in the ON state array is the P2 domain of phosphorylated CheA, with a second minor site being the carboxyl-terminal pentapeptide of the serine chemoreceptor. Thus, we have been able to quantify the regulation of the signal output of the receptor array by the intracellular dynamics of an adaptation enzyme.
出版者	American Association for the Advancement of Science
収録物名	Science Advances
巻(号)	10(38)
ページ	eadp5636
刊行年月	2024-09-20
言語	英語
ハンドルURL	https://hdl.handle.net/11094/98253
eISSN	23752548
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1126/sciadv.adp5636
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Science Advances. 2024, 10(38), p. eadp5636
DC.title	CheB localizes to polar receptor arrays during repellent adaptation
DC.creator	Fukuoka, Hajime
	Nishitani, Keisuke
	Deguchi, Taiga
	Oshima, Taketo
	Uchida, Yumiko
	Hamamoto, Tatsuki
	Che, Yong Suk
	Ishijima, Akihiko
DC.publisher	American Association for the Advancement of Science
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-09-20
DC.identifier	https://doi.org/10.1126/sciadv.adp5636
DC.description	Fukuoka H., Nishitani K., Deguchi T., et al. CheB localizes to polar receptor arrays during repellent adaptation. Science Advances 10, eadp5636 (2024); https://doi.org/10.1126/sciadv.adp5636.
DCTERMS.abstract	Adaptation of the response to stimuli is a fundamental process for all organisms. Here, we show that the adaptation enzyme CheB methylesterase of Escherichia coli assembles to the ON state receptor array after exposure to the repellent l-isoleucine and dissociates from the array after adaptation is complete. The duration of increased CheB localization and the time of highly clockwise-biased flagellar rotation were similar and depended on the strength of the stimulus. The increase in CheB at the receptor array and the decrease in cytoplasmic CheB were both ~100 molecules, which represents 15 to 20% of the total cellular content of CheB. We confirmed that the main binding site for CheB in the ON state array is the P2 domain of phosphorylated CheA, with a second minor site being the carboxyl-terminal pentapeptide of the serine chemoreceptor. Thus, we have been able to quantify the regulation of the signal output of the receptor array by the intracellular dynamics of an adaptation enzyme.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	98253
citation_title	CheB localizes to polar receptor arrays during repellent adaptation
citation_author	Fukuoka, Hajime
	Nishitani, Keisuke
	Deguchi, Taiga
	Oshima, Taketo
	Uchida, Yumiko
	Hamamoto, Tatsuki
	Che, Yong Suk
	Ishijima, Akihiko
citation_publisher	American Association for the Advancement of Science
citation_language	英語
citation_date	2024-09-20
citation_journal_title	Science Advances
citation_volume	10
citation_issue	38
citation_firstpage	eadp5636
citation_doi	https://doi.org/10.1126/sciadv.adp5636
citation_public_url	https://hdl.handle.net/11094/98253

CheB localizes to polar receptor arrays during repellent adaptation

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