Robust tissue pattern formation by coupling morphogen signal and cell adhesion

Mizuno, Kosuke; Hirashima, Tsuyoshi; Toda, Satoshi

doi:https://doi.org/10.1038/s44319-024-00261-z

Robust tissue pattern formation by coupling morphogen signal and cell adhesion

Mizuno, Kosuke; Hirashima, Tsuyoshi; Toda, Satoshi

EMBO Reports, 2024, 25, 4803-4826

アクセス数:384件（2026-02-15 21:10 集計）

固定URL: https://hdl.handle.net/11094/98357

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
EMBORep_s44319-024-00261-z	pdf	なし	10.6 MB	182	2024.10.29

Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	Robust tissue pattern formation by coupling morphogen signal and cell adhesion
著者	researchmap permalink rice-eater rice-eater 著者名 Mizuno, Kosuke
	ORCID 0000-0001-7323-9627 0000-0001-7323-9627 著者名 Hirashima, Tsuyoshi
	NRID 1000020738835 1000020738835 researchmap permalink satoshi.toda satoshi.toda Scopus Author ID 57202382051 57202382051 著者名 Toda, Satoshi
キーワード等	Cadherin
	Cell Adhesion
	Morphogen
	Pattern Formation
	Synthetic Biology
内容	Mizuno K., Hirashima T., Toda S. Robust tissue pattern formation by coupling morphogen signal and cell adhesion. EMBO Reports, (2024); https://doi.org/10.1038/s44319-024-00261-z.
抄録	Morphogens, locally produced signaling molecules, form a concentration gradient to guide tissue patterning. Tissue patterns emerge as a collaboration between morphogen diffusion and responsive cell behaviors, but the mechanisms through which diffusing morphogens define precise spatial patterns amidst biological fluctuations remain unclear. To investigate how cells respond to diffusing proteins to generate tissue patterns, we develop SYMPLE3D, a 3D culture platform. By engineering gene expression responsive to artificial morphogens, we observe that coupling morphogen signals with cadherin-based adhesion is sufficient to convert a morphogen gradient into distinct tissue domains. Morphogen-induced cadherins gather activated cells into a single domain, removing ectopically activated cells. In addition, we reveal a switch-like induction of cadherin-mediated compaction and cell mixing, homogenizing activated cells within the morphogen gradient to form a uniformly activated domain with a sharp boundary. These findings highlight the cooperation between morphogen gradients and cell adhesion in robust tissue patterning and introduce a novel method for tissue engineering to develop new tissue domains in organoids.
出版者	EMBO Press
収録物名	EMBO Reports
巻(号)	25
ページ	4803-4826
刊行年月	2024-09-27
言語	英語
ハンドルURL	https://hdl.handle.net/11094/98357
PISSN	1469221X
eISSN	14693178
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s44319-024-00261-z
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	EMBO Reports. 2024, 25, p. 4803-4826
DC.title	Robust tissue pattern formation by coupling morphogen signal and cell adhesion
DC.creator	Mizuno, Kosuke
	Hirashima, Tsuyoshi
	Toda, Satoshi
DC.publisher	EMBO Press
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-09-27
DC.identifier	https://doi.org/10.1038/s44319-024-00261-z
DC.subject	Cadherin
	Cell Adhesion
	Morphogen
	Pattern Formation
	Synthetic Biology
DC.description	Mizuno K., Hirashima T., Toda S. Robust tissue pattern formation by coupling morphogen signal and cell adhesion. EMBO Reports, (2024); https://doi.org/10.1038/s44319-024-00261-z.
DCTERMS.abstract	Morphogens, locally produced signaling molecules, form a concentration gradient to guide tissue patterning. Tissue patterns emerge as a collaboration between morphogen diffusion and responsive cell behaviors, but the mechanisms through which diffusing morphogens define precise spatial patterns amidst biological fluctuations remain unclear. To investigate how cells respond to diffusing proteins to generate tissue patterns, we develop SYMPLE3D, a 3D culture platform. By engineering gene expression responsive to artificial morphogens, we observe that coupling morphogen signals with cadherin-based adhesion is sufficient to convert a morphogen gradient into distinct tissue domains. Morphogen-induced cadherins gather activated cells into a single domain, removing ectopically activated cells. In addition, we reveal a switch-like induction of cadherin-mediated compaction and cell mixing, homogenizing activated cells within the morphogen gradient to form a uniformly activated domain with a sharp boundary. These findings highlight the cooperation between morphogen gradients and cell adhesion in robust tissue patterning and introduce a novel method for tissue engineering to develop new tissue domains in organoids.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	98357
citation_title	Robust tissue pattern formation by coupling morphogen signal and cell adhesion
citation_author	Mizuno, Kosuke
	Hirashima, Tsuyoshi
	Toda, Satoshi
citation_publisher	EMBO Press
citation_language	英語
citation_date	2024-09-27
citation_journal_title	EMBO Reports
citation_volume	25
citation_firstpage	4803
citation_lastpage	4826
citation_doi	https://doi.org/10.1038/s44319-024-00261-z
citation_public_url	https://hdl.handle.net/11094/98357

Robust tissue pattern formation by coupling morphogen signal and cell adhesion

閲覧可能ファイル

論文情報

論文詳細を表示