Influence of bone morphogenetic protein (BMP) signaling and masticatory load on morphological alterations of the mouse mandible during postnatal development

Uptegrove, Amber; Chen, Coral; Sahagun-Bisson, Madison; Kulkarni, Anshul K.; Louie, Ke'ale W.; Ueharu, Hiroki; Mishina, Yuji; Omi-Sugihara, Maiko

doi:https://doi.org/10.1016/j.archoralbio.2024.106096

Influence of bone morphogenetic protein (BMP) signaling and masticatory load on morphological alterations of the mouse mandible during postnatal development

Uptegrove, Amber; Chen, Coral; Sahagun-Bisson, Madison et al.

Archives of Oral Biology, 2025, 169, 106096

アクセス数:485件（2026-02-15 22:22 集計）

固定URL: https://hdl.handle.net/11094/98437

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タイトル	Influence of bone morphogenetic protein (BMP) signaling and masticatory load on morphological alterations of the mouse mandible during postnatal development
著者	著者名 Uptegrove, Amber
	著者名 Chen, Coral
	著者名 Sahagun-Bisson, Madison
	著者名 Kulkarni, Anshul K.
	著者名 Louie, Ke'ale W.
	著者名 Ueharu, Hiroki
	著者名 Mishina, Yuji
	ORCID 0000-0002-1347-9044 0000-0002-1347-9044 researchmap permalink maikosugihara maikosugihara 著者名 Omi-Sugihara, Maiko
キーワード等	BMP
	Condyle
	Mandible
	Mastication
	Mechanical loading
	Soft diet
内容	Uptegrove A., Chen C., Sahagun-Bisson M., et al. Influence of bone morphogenetic protein (BMP) signaling and masticatory load on morphological alterations of the mouse mandible during postnatal development. Archives of Oral Biology 169, 106096 (2025); https://doi.org/10.1016/j.archoralbio.2024.106096.
抄録	Objective: Bone homeostasis relies on several contributing factors, encompassing growth factors and mechanical stimuli. While bone morphogenetic protein (BMP) signaling is acknowledged for its essential role in skeletal development, its specific impact on mandibular morphogenesis remains unexplored. Here, we investigated the involvement of BMP signaling and mechanical loading through mastication in postnatal mandibular morphogenesis. Design: We employed conditional deletion of Bmpr1a in osteoblasts and chondrocytes via Osterix-Cre. Cre activity was induced at birth for the 3-week group and at three weeks for the 9-week and 12-week groups, respectively. The conditional knockout (cKO) and control mice were given either a regular diet (hard diet, HD) or a powdered diet (soft diet, SD) from 3 weeks until sample collection, followed by micro-CT and histological analysis. Results: The cKO mice exhibited shorter anterior lengths and a posteriorly inclined ramus across all age groups compared to the control mice. The cKO mice displayed an enlarged hypertrophic cartilage area along with fewer osteoclast numbers in the subchondral bone of the condyle compared to the control group at three weeks, followed by a reduction in the cartilage area in the posterior region at twelve weeks. Superimposed imaging and histomorphometrical analysis of the condyle revealed that BMP signaling primarily affects the posterior part of the condyle, while mastication affects the anterior part. Conclusions: Using 3D landmark-based geometric morphometrics and histological assessments of the mandible, we demonstrated that BMP signaling and mechanical loading reciprocally contribute to the morphological alterations of the mandible and condyle during postnatal development.
出版者	Elsevier Ltd
収録物名	Archives of Oral Biology
巻(号)	169
ページ	106096
刊行年月	2025-01-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/98437
PISSN	00039969
eISSN	18791506
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.archoralbio.2024.106096
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Archives of Oral Biology. 2025, 169, p. 106096
DC.title	Influence of bone morphogenetic protein (BMP) signaling and masticatory load on morphological alterations of the mouse mandible during postnatal development
DC.creator	Uptegrove, Amber
	Chen, Coral
	Sahagun-Bisson, Madison
	Kulkarni, Anshul K.
	Louie, Ke'ale W.
	Ueharu, Hiroki
	Mishina, Yuji
	Omi-Sugihara, Maiko
DC.publisher	Elsevier Ltd
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-01-01
DC.identifier	https://doi.org/10.1016/j.archoralbio.2024.106096
DC.subject	BMP
	Condyle
	Mandible
	Mastication
	Mechanical loading
	Soft diet
DC.description	Uptegrove A., Chen C., Sahagun-Bisson M., et al. Influence of bone morphogenetic protein (BMP) signaling and masticatory load on morphological alterations of the mouse mandible during postnatal development. Archives of Oral Biology 169, 106096 (2025); https://doi.org/10.1016/j.archoralbio.2024.106096.
DCTERMS.abstract	Objective: Bone homeostasis relies on several contributing factors, encompassing growth factors and mechanical stimuli. While bone morphogenetic protein (BMP) signaling is acknowledged for its essential role in skeletal development, its specific impact on mandibular morphogenesis remains unexplored. Here, we investigated the involvement of BMP signaling and mechanical loading through mastication in postnatal mandibular morphogenesis. Design: We employed conditional deletion of Bmpr1a in osteoblasts and chondrocytes via Osterix-Cre. Cre activity was induced at birth for the 3-week group and at three weeks for the 9-week and 12-week groups, respectively. The conditional knockout (cKO) and control mice were given either a regular diet (hard diet, HD) or a powdered diet (soft diet, SD) from 3 weeks until sample collection, followed by micro-CT and histological analysis. Results: The cKO mice exhibited shorter anterior lengths and a posteriorly inclined ramus across all age groups compared to the control mice. The cKO mice displayed an enlarged hypertrophic cartilage area along with fewer osteoclast numbers in the subchondral bone of the condyle compared to the control group at three weeks, followed by a reduction in the cartilage area in the posterior region at twelve weeks. Superimposed imaging and histomorphometrical analysis of the condyle revealed that BMP signaling primarily affects the posterior part of the condyle, while mastication affects the anterior part. Conclusions: Using 3D landmark-based geometric morphometrics and histological assessments of the mandible, we demonstrated that BMP signaling and mechanical loading reciprocally contribute to the morphological alterations of the mandible and condyle during postnatal development.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	98437
citation_title	Influence of bone morphogenetic protein (BMP) signaling and masticatory load on morphological alterations of the mouse mandible during postnatal development
citation_author	Uptegrove, Amber
	Chen, Coral
	Sahagun-Bisson, Madison
	Kulkarni, Anshul K.
	Louie, Ke'ale W.
	Ueharu, Hiroki
	Mishina, Yuji
	Omi-Sugihara, Maiko
citation_publisher	Elsevier Ltd
citation_language	英語
citation_date	2025-01-01
citation_journal_title	Archives of Oral Biology
citation_volume	169
citation_firstpage	106096
citation_doi	https://doi.org/10.1016/j.archoralbio.2024.106096
citation_public_url	https://hdl.handle.net/11094/98437

Influence of bone morphogenetic protein (BMP) signaling and masticatory load on morphological alterations of the mouse mandible during postnatal development

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