Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction

Zhang, Jingbo; Li, Junjun; Qu, Xiang; Liu, Yuting; Sun, Lifu; Harada, Akima; Hua, Ying; Sougawa, Nagako; Tabata, Akiko; Liu, Li; Miyagawa, Shigeru

doi:https://doi.org/10.1016/j.bioactmat.2024.03.028

Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction

Zhang, Jingbo; Li, Junjun; Qu, Xiang et al.

Bioactive Materials, 2024, 37, 533-548

アクセス数:34件（2025-04-27 00:17 集計）

固定URL: https://hdl.handle.net/11094/98538

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タイトル	Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction
著者	著者名 Zhang, Jingbo
	NRID 1000010723786 1000010723786 ORCID 0000-0002-7473-4918 0000-0002-7473-4918 researchmap permalink liJJ liJJ Scopus Author ID 52464043600 52464043600 著者名 Li, Junjun
	著者名 Qu, Xiang
	著者名 Liu, Yuting
	著者名 Sun, Lifu
	NRID 1000070535426 1000070535426 著者名 Harada, Akima
	著者名 Hua, Ying
	NRID 1000030432579 1000030432579 researchmap permalink SougawaNagako SougawaNagako 著者名 Sougawa, Nagako
	著者名 Tabata, Akiko
	NRID 1000050380093 1000050380093 researchmap permalink 7000008694 7000008694 Scopus Author ID 55628530013 55628530013 著者名 Liu, Li
	NRID 1000070544237 1000070544237 ORCID 0000-0003-0015-6569 0000-0003-0015-6569 researchmap permalink s-miyagawa3154 s-miyagawa3154 Scopus Author ID 55560624700 55560624700 著者名 Miyagawa, Shigeru
キーワード等	Human adipose-derived mesenchymal stem cell
	Human induced pluripotent stem cell-derived cardiomyocyte
	Myocardial infarction
	Regenerative medicine
	Tissue engineering
内容	Zhang J., Li J., Qu X., et al. Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction. Bioactive Materials 37, 533 (2024); https://doi.org/10.1016/j.bioactmat.2024.03.028.
抄録	Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been widely used in therapy of ischemic heart disease. However, there are still remaining issues that limit the therapeutic efficacy, such as immune rejection and low retention of hiPSC-CMs. Human adipose mesenchymal stromal cells (hADSCs) have been reported to be able to regulate the immune response, promote angiogenesis and promote the maturation of hiPSC-CMs. In this study, we co-cultured these two types of cells on fiber scaffold made of biodegradable poly (D,L-lactic-co-glycolic acid) (PLGA) polymer for several days to develop a composited 3D cardiac tissue sheet. As expected, the cells formed 231.00 ± 15.14 μm thickness tissue, with improved organization, alignment, ECM condition, contractile ability, and paracrine function compared to culture hiPSC-CMs only on PLGA fiber. Furthermore, the composited 3D cardiac tissue sheet significantly promoted the engraftment and survival after transplantation. The composited 3D cardiac tissue sheet also increased cardiac function, attenuated ventricular remodeling, decreased fibrosis, and enhanced angiogenesis in rat myocardial infarction model, indicating that this strategy wound be a promising therapeutic option in the clinical scenario.
出版者	Elsevier B.V.
収録物名	Bioactive Materials
巻(号)	37
ページ	533-548
刊行年月	2024-07-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/98538
PISSN	2452199X
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.bioactmat.2024.03.028
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Bioactive Materials. 2024, 37, p. 533-548
DC.title	Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction
DC.creator	Zhang, Jingbo
	Li, Junjun
	Qu, Xiang
	Liu, Yuting
	Sun, Lifu
	Harada, Akima
	Hua, Ying
	Sougawa, Nagako
	Tabata, Akiko
	Liu, Li
	Miyagawa, Shigeru
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-07-01
DC.identifier	https://doi.org/10.1016/j.bioactmat.2024.03.028
DC.subject	Human adipose-derived mesenchymal stem cell
	Human induced pluripotent stem cell-derived cardiomyocyte
	Myocardial infarction
	Regenerative medicine
	Tissue engineering
DC.description	Zhang J., Li J., Qu X., et al. Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction. Bioactive Materials 37, 533 (2024); https://doi.org/10.1016/j.bioactmat.2024.03.028.
DCTERMS.abstract	Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have been widely used in therapy of ischemic heart disease. However, there are still remaining issues that limit the therapeutic efficacy, such as immune rejection and low retention of hiPSC-CMs. Human adipose mesenchymal stromal cells (hADSCs) have been reported to be able to regulate the immune response, promote angiogenesis and promote the maturation of hiPSC-CMs. In this study, we co-cultured these two types of cells on fiber scaffold made of biodegradable poly (D,L-lactic-co-glycolic acid) (PLGA) polymer for several days to develop a composited 3D cardiac tissue sheet. As expected, the cells formed 231.00 ± 15.14 μm thickness tissue, with improved organization, alignment, ECM condition, contractile ability, and paracrine function compared to culture hiPSC-CMs only on PLGA fiber. Furthermore, the composited 3D cardiac tissue sheet significantly promoted the engraftment and survival after transplantation. The composited 3D cardiac tissue sheet also increased cardiac function, attenuated ventricular remodeling, decreased fibrosis, and enhanced angiogenesis in rat myocardial infarction model, indicating that this strategy wound be a promising therapeutic option in the clinical scenario.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	98538
citation_title	Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction
citation_author	Zhang, Jingbo
	Li, Junjun
	Qu, Xiang
	Liu, Yuting
	Sun, Lifu
	Harada, Akima
	Hua, Ying
	Sougawa, Nagako
	Tabata, Akiko
	Liu, Li
	Miyagawa, Shigeru
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2024-07-01
citation_journal_title	Bioactive Materials
citation_volume	37
citation_firstpage	533
citation_lastpage	548
citation_doi	https://doi.org/10.1016/j.bioactmat.2024.03.028
citation_public_url	https://hdl.handle.net/11094/98538

Development of composite functional tissue sheets using hiPSC-CMs and hADSCs to improve the cardiac function after myocardial infarction

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