Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts

Kusuma, Subhan Hadi; Kakizuka, Taishi; Hattori, Mitsuru; Nagai, Takeharu

doi:https://doi.org/10.1073/pnas.2406358121

Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts

Kusuma, Subhan Hadi; Kakizuka, Taishi; Hattori, Mitsuru et al.

Proceedings of the National Academy of Sciences of the United States of America, 2024, 121(41), e2406358121

アクセス数:493件（2025-07-18 11:41 集計）

固定URL: https://hdl.handle.net/11094/98540

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タイトル	Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts
著者	著者名 Kusuma, Subhan Hadi
	ORCID 0000-0003-4269-5923 0000-0003-4269-5923 researchmap permalink t-kakizuka t-kakizuka 著者名 Kakizuka, Taishi
	NRID 1000020589858 1000020589858 researchmap permalink mhatto mhatto Scopus Author ID 7401882679 7401882679 ORCID 0000-0003-1189-6554 0000-0003-1189-6554 著者名 Hattori, Mitsuru
	NRID 1000020311350 1000020311350 ORCID 0000-0003-2650-9895 0000-0003-2650-9895 researchmap permalink ng1 ng1 Scopus Author ID 7403433377 7403433377 著者名 Nagai, Takeharu
キーワード等	bacterial luciferase
	bioimaging
	bioluminescence
	biosensor
内容	Kusuma S.H., Kakizuka T., Hattori M., et al. Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts. Proceedings of the National Academy of Sciences of the United States of America 121, e2406358121 (2024); https://doi.org/10.1073/pnas.2406358121.
抄録	Bioluminescence imaging has become a valuable tool in biological research, offering several advantages over fluorescence-based techniques, including the absence of phototoxicity and photobleaching, along with a higher signal-to-noise ratio. Common bioluminescence imaging methods often require the addition of an external chemical substrate (luciferin), which can result in a decrease in luminescence intensity over time and limit prolonged observations. Since the bacterial bioluminescence system is genetically encoded for luciferase-luciferin production, it enables autonomous bioluminescence (auto-bioluminescence) imaging. However, its application to multiple reporters is restricted due to a limited range of color variants. Here, we report five-color auto-bioluminescence system named Nano-lanternX (NLX), which can be expressed in bacterial, mammalian, and plant hosts, thereby enabling auto-bioluminescence in various living organisms. Utilizing spectral unmixing, we achieved the successful observation of multicolor auto-bioluminescence, enabling detailed single-cell imaging across both bacterial and mammalian cells. We have also expanded the applications of the NLX system, such as multiplexed auto-bioluminescence imaging for gene expression, protein localization, and dynamics of biomolecules within living mammalian cells.
出版者	National Academy of Sciences
収録物名	Proceedings of the National Academy of Sciences of the United States of America
巻(号)	121(41)
ページ	e2406358121
刊行年月	2024-10-08
言語	英語
ハンドルURL	https://hdl.handle.net/11094/98540
PISSN	00278424
eISSN	10916490
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1073/pnas.2406358121
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Proceedings of the National Academy of Sciences of the United States of America. 2024, 121(41), p. e2406358121
DC.title	Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts
DC.creator	Kusuma, Subhan Hadi
	Kakizuka, Taishi
	Hattori, Mitsuru
	Nagai, Takeharu
DC.publisher	National Academy of Sciences
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-10-08
DC.identifier	https://doi.org/10.1073/pnas.2406358121
DC.subject	bacterial luciferase
	bioimaging
	bioluminescence
	biosensor
DC.description	Kusuma S.H., Kakizuka T., Hattori M., et al. Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts. Proceedings of the National Academy of Sciences of the United States of America 121, e2406358121 (2024); https://doi.org/10.1073/pnas.2406358121.
DCTERMS.abstract	Bioluminescence imaging has become a valuable tool in biological research, offering several advantages over fluorescence-based techniques, including the absence of phototoxicity and photobleaching, along with a higher signal-to-noise ratio. Common bioluminescence imaging methods often require the addition of an external chemical substrate (luciferin), which can result in a decrease in luminescence intensity over time and limit prolonged observations. Since the bacterial bioluminescence system is genetically encoded for luciferase-luciferin production, it enables autonomous bioluminescence (auto-bioluminescence) imaging. However, its application to multiple reporters is restricted due to a limited range of color variants. Here, we report five-color auto-bioluminescence system named Nano-lanternX (NLX), which can be expressed in bacterial, mammalian, and plant hosts, thereby enabling auto-bioluminescence in various living organisms. Utilizing spectral unmixing, we achieved the successful observation of multicolor auto-bioluminescence, enabling detailed single-cell imaging across both bacterial and mammalian cells. We have also expanded the applications of the NLX system, such as multiplexed auto-bioluminescence imaging for gene expression, protein localization, and dynamics of biomolecules within living mammalian cells.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	98540
citation_title	Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts
citation_author	Kusuma, Subhan Hadi
	Kakizuka, Taishi
	Hattori, Mitsuru
	Nagai, Takeharu
citation_publisher	National Academy of Sciences
citation_language	英語
citation_date	2024-10-08
citation_journal_title	Proceedings of the National Academy of Sciences of the United States of America
citation_volume	121
citation_issue	41
citation_firstpage	e2406358121
citation_doi	https://doi.org/10.1073/pnas.2406358121
citation_public_url	https://hdl.handle.net/11094/98540

Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts

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