学術雑誌論文

学術雑誌論文

Nucleic Acids Research. 2024, 52(22), p. 13530-13543

Profiling of i-motif-binding proteins reveals functional roles of nucleolin in regulation of high-order DNA structures

Oxford University Press (OUP)

英語

2024-11-19

https://doi.org/10.1093/nar/gkae1001

Ban Yuki, Ando Yuka, Terai Yuma, et al. Profiling of i-motif-binding proteins reveals functional roles of nucleolin in regulation of high-order DNA structures. Nucleic Acids Research 48, 2013 (2024); https://doi.org/10.1093/nar/gkae1001.

Non-canonical DNA structures, such as the G-quadruplex (G4) and i-motif (iM), are formed at guanine- and cytosine-rich sequences, respectively, in living cells and involved in regulating various biological processes during the cell cycle. Therefore, the formation and resolution of these non-canonical structures must be dynamically regulated by physiological conditions or factors that can bind G4 and iM structures. Although many G4 binding proteins responsible for tuning the G4 structure have been discovered, the structural regulation of iM by iM-binding proteins remains enigmatic. In this study, we developed a protein-labeling DNA probe bearing an alkyne moiety through a reactive linker, for proximity-labeling of nucleic acid-binding proteins, and searched for new iM-binding proteins. Alkyne-modified proteins in the nuclear extract of HeLa cells were labeled with biotin via a click reaction and then captured with streptavidin-coated magnetic beads. This fingerprint-targeting enrichment, followed by proteome analyses, identified new candidate proteins that potentially bind to the iM structure, in addition to the reported iM-binding proteins. Among the newly identified candidates, we characterized a nucleolar protein, nucleolin, that binds to the iM structure and relaxes it, while nucleolin stabilizes the G4 structure.

This article is licensed under a Creative Commons Attribution 4.0 International License.

98550

Profiling of i-motif-binding proteins reveals functional roles of nucleolin in regulation of high-order DNA structures

Oxford University Press (OUP)

英語

2024-11-19

Nucleic Acids Research

52

22

13530

13543

https://doi.org/10.1093/nar/gkae1001

https://hdl.handle.net/11094/98550