Variability in non-tumor areas of colorectal cancer patients as revealed by endoscopic intestinal step biopsies

Ikuta, Shoko; Saito, Yutaka; Takata, So; Nakatani, Yoichiro; Nagatomo, Izumi; Shiba, Satoshi; Takeda, Yoshito; Totoki, Yasushi; Mizutani, Sayaka; Sunakawa, Hironori; Ikematsu, Hiroaki; Takamaru, Hiroyuki; Kumanogoh, Atsushi; Yachida, Shinichi

doi:https://doi.org/10.1186/s12943-024-02159-9

Variability in non-tumor areas of colorectal cancer patients as revealed by endoscopic intestinal step biopsies

Ikuta, Shoko; Saito, Yutaka; Takata, So et al.

Molecular Cancer, 2024, 23, 249

アクセス数:41件（2025-04-06 21:14 集計）

固定URL: https://hdl.handle.net/11094/98555

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タイトル	Variability in non-tumor areas of colorectal cancer patients as revealed by endoscopic intestinal step biopsies
著者	著者名 Ikuta, Shoko
	NRID 1000090501859 1000090501859 著者名 Saito, Yutaka
	著者名 Takata, So
	NRID 1000010847891 1000010847891 ORCID 0000-0001-7604-0242 0000-0001-7604-0242 researchmap permalink nakatani_yoichiro nakatani_yoichiro Scopus Author ID 8534216500 8534216500 著者名 Nakatani, Yoichiro
	NRID 1000010570583 1000010570583 researchmap permalink saxon-w saxon-w Scopus Author ID 13404305500 13404305500 著者名 Nagatomo, Izumi
	NRID 1000000797003 1000000797003 著者名 Shiba, Satoshi
	NRID 1000040452388 1000040452388 researchmap permalink yoshi10 yoshi10 Scopus Author ID 7404001848 7404001848 著者名 Takeda, Yoshito
	NRID 1000010373333 1000010373333 researchmap permalink ytotoki ytotoki Scopus Author ID 58966284600 58966284600 著者名 Totoki, Yasushi
	NRID 1000070790278 1000070790278 著者名 Mizutani, Sayaka
	NRID 1000050844792 1000050844792 著者名 Sunakawa, Hironori
	著者名 Ikematsu, Hiroaki
	著者名 Takamaru, Hiroyuki
	NRID 1000010294125 1000010294125 researchmap permalink read0051725 read0051725 Scopus Author ID 6602726311 6602726311 ORCID 0000-0003-4749-7117 0000-0003-4749-7117 著者名 Kumanogoh, Atsushi
	NRID 1000020359920 1000020359920 researchmap permalink read0210591 read0210591 Scopus Author ID 57226203270 57226203270 著者名 Yachida, Shinichi
キーワード等	Adenoma
	CIBERSORTx
	Colorectal cancer
	Field cancerization
	Immune cell
	Left-sided colorectum
	Right-sided colon
	Terminal ileum
	Transcriptome
内容	Ikuta S., Saito Y., Takata S., et al. Variability in non-tumor areas of colorectal cancer patients as revealed by endoscopic intestinal step biopsies. Molecular Cancer 23, 249 (2024); https://doi.org/10.1186/s12943-024-02159-9.
抄録	A comprehensive endoscopic small and large intestinal untargeted step biopsy procedure was conducted to compare gene expression between the normal intestinal mucosa of healthy individuals and that of patients with colorectal tumors. From 78 participants (healthy individuals [n = 17], patients with colorectal conventional adenomas [n = 6], patients with Tis–T1 colorectal cancer [n = 41], patients with T2–4 colorectal cancer [n = 14]), biopsies of normal mucosa of the terminal ileum, right-sided colon (cecum and ascending colon), and left-sided colorectum (descending colon, sigmoid colon, and rectum) were obtained using a lower gastrointestinal endoscope. RNA was extracted from all samples, and total transcriptome sequencing was performed. Transcriptome data from 388 samples was analyzed. DNA was also extracted from tumor biopsy tissues and analyzed for whole-exome sequencing. In healthy individuals, gene expression differed significantly among the terminal ileum, right-sided colon, and left-sided colorectum, presumably linked to embryological factors. There were differences in gene expression in the normal mucosa in colorectal cancer patients, compared to healthy controls. Patients with tumors, especially T2–4 colorectal cancer, showed considerable variation in gene expression in non-tumor tissues, even in the terminal ileum distant from the tumor site. Based on endoscopic biopsies, the results imply cancer-predisposing conditions in seemingly normal tissues. The present study points to the importance of small intestine and cancer-predisposing conditions in the colon of colorectal cancer patients, with possible implications for developing novel immunotherapy and other therapeutic modalities.
出版者	BioMed Central Ltd
収録物名	Molecular Cancer
巻(号)	23
ページ	249
刊行年月	2024-11-07
言語	英語
ハンドルURL	https://hdl.handle.net/11094/98555
eISSN	14764598
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1186/s12943-024-02159-9
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Molecular Cancer. 2024, 23, p. 249
DC.title	Variability in non-tumor areas of colorectal cancer patients as revealed by endoscopic intestinal step biopsies
DC.creator	Ikuta, Shoko
	Saito, Yutaka
	Takata, So
	Nakatani, Yoichiro
	Nagatomo, Izumi
	Shiba, Satoshi
	Takeda, Yoshito
	Totoki, Yasushi
	Mizutani, Sayaka
	Sunakawa, Hironori
	Ikematsu, Hiroaki
	Takamaru, Hiroyuki
	Kumanogoh, Atsushi
	Yachida, Shinichi
DC.publisher	BioMed Central Ltd
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-11-07
DC.identifier	https://doi.org/10.1186/s12943-024-02159-9
DC.subject	Adenoma
	CIBERSORTx
	Colorectal cancer
	Field cancerization
	Immune cell
	Left-sided colorectum
	Right-sided colon
	Terminal ileum
	Transcriptome
DC.description	Ikuta S., Saito Y., Takata S., et al. Variability in non-tumor areas of colorectal cancer patients as revealed by endoscopic intestinal step biopsies. Molecular Cancer 23, 249 (2024); https://doi.org/10.1186/s12943-024-02159-9.
DCTERMS.abstract	A comprehensive endoscopic small and large intestinal untargeted step biopsy procedure was conducted to compare gene expression between the normal intestinal mucosa of healthy individuals and that of patients with colorectal tumors. From 78 participants (healthy individuals [n = 17], patients with colorectal conventional adenomas [n = 6], patients with Tis–T1 colorectal cancer [n = 41], patients with T2–4 colorectal cancer [n = 14]), biopsies of normal mucosa of the terminal ileum, right-sided colon (cecum and ascending colon), and left-sided colorectum (descending colon, sigmoid colon, and rectum) were obtained using a lower gastrointestinal endoscope. RNA was extracted from all samples, and total transcriptome sequencing was performed. Transcriptome data from 388 samples was analyzed. DNA was also extracted from tumor biopsy tissues and analyzed for whole-exome sequencing. In healthy individuals, gene expression differed significantly among the terminal ileum, right-sided colon, and left-sided colorectum, presumably linked to embryological factors. There were differences in gene expression in the normal mucosa in colorectal cancer patients, compared to healthy controls. Patients with tumors, especially T2–4 colorectal cancer, showed considerable variation in gene expression in non-tumor tissues, even in the terminal ileum distant from the tumor site. Based on endoscopic biopsies, the results imply cancer-predisposing conditions in seemingly normal tissues. The present study points to the importance of small intestine and cancer-predisposing conditions in the colon of colorectal cancer patients, with possible implications for developing novel immunotherapy and other therapeutic modalities.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	98555
citation_title	Variability in non-tumor areas of colorectal cancer patients as revealed by endoscopic intestinal step biopsies
citation_author	Ikuta, Shoko
	Saito, Yutaka
	Takata, So
	Nakatani, Yoichiro
	Nagatomo, Izumi
	Shiba, Satoshi
	Takeda, Yoshito
	Totoki, Yasushi
	Mizutani, Sayaka
	Sunakawa, Hironori
	Ikematsu, Hiroaki
	Takamaru, Hiroyuki
	Kumanogoh, Atsushi
	Yachida, Shinichi
citation_publisher	BioMed Central Ltd
citation_language	英語
citation_date	2024-11-07
citation_journal_title	Molecular Cancer
citation_volume	23
citation_firstpage	249
citation_doi	https://doi.org/10.1186/s12943-024-02159-9
citation_public_url	https://hdl.handle.net/11094/98555

Variability in non-tumor areas of colorectal cancer patients as revealed by endoscopic intestinal step biopsies

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