学術雑誌論文

学術雑誌論文

iScience. 2024, 27(11), p. 111214

Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis

Elsevier Inc.

英語

2024-11-15

https://doi.org/10.1016/j.isci.2024.111214

Fukuda M., Okanishi H., Ino D., et al. Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis. iScience 27, 111214 (2024); https://doi.org/10.1016/j.isci.2024.111214.

The cochlea contains two extracellular fluids, perilymph and endolymph. Endolymph exhibits high potential of approximately +80 to +110 mV (depending on species), which sensitizes sensory hair cells. Other properties of this unique fluid remain elusive, owing to its minuscule volume in rodent cochlea. We therefore developed a technique to collect high-purity endolymph from mouse cochleae. Comprehensive proteomic analysis of sampled endolymph using liquid chromatography with mass spectrometry identified 301 proteins, dominated by molecules engaged in immunity and proteostasis. Approximately 30% of these proteins were undetectable in our perilymph. A combination of mass spectrometry and different approaches revealed that, compared to perilymph, endolymph was enriched with α2-macroglobulin, osteopontin, apolipoprotein D, apolipoprotein E, and apolipoprotein J/clusterin. In other cells or tissues, α2-macroglobulin, apolipoprotein E, and apolipoprotein J contribute to the clearance of degraded proteins from extracellular fluid. Altogether, with the proteins described here, endolymph may play a protective role in stabilizing cochlear homeostasis.

This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

98558

Protein profile of mouse endolymph suggests a role in controlling cochlear homeostasis

Elsevier Inc.

英語

2024-11-15

iScience

27

11

111214

https://doi.org/10.1016/j.isci.2024.111214

https://hdl.handle.net/11094/98558