Improved ion detection sensitivity in mass spectrometry imaging using tapping-mode scanning probe electrospray ionization to visualize localized lipids in mouse testes

Otsuka, Yoichi; Okada, Maki; Hashidate-Yoshida, Tomomi; Nagata, Katsuyuki; Yamada, Makoto; Goto, Motohito; Sun, Mengze; Shindou, Hideo; Toyoda, Michisato

doi:https://doi.org/10.1007/s00216-024-05641-x

Improved ion detection sensitivity in mass spectrometry imaging using tapping-mode scanning probe electrospray ionization to visualize localized lipids in mouse testes

Otsuka, Yoichi; Okada, Maki; Hashidate-Yoshida, Tomomi et al.

Analytical and Bioanalytical Chemistry, 2024, 417(2), 275-286

アクセス数:1,129件（2025-08-29 20:04 集計）

固定URL: https://hdl.handle.net/11094/98817

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タイトル	Improved ion detection sensitivity in mass spectrometry imaging using tapping-mode scanning probe electrospray ionization to visualize localized lipids in mouse testes
著者	NRID 1000070756460 1000070756460 researchmap permalink yoichiotsuka yoichiotsuka Scopus Author ID 55181504000 55181504000 ORCID 0000-0003-2304-6637 0000-0003-2304-6637 著者名 Otsuka, Yoichi
	著者名 Okada, Maki
	著者名 Hashidate-Yoshida, Tomomi
	著者名 Nagata, Katsuyuki
	著者名 Yamada, Makoto
	NRID 1000020469985 1000020469985 著者名 Goto, Motohito
	著者名 Sun, Mengze
	NRID 1000010401027 1000010401027 著者名 Shindou, Hideo
	NRID 1000080283828 1000080283828 ORCID 0000-0001-5950-6039 0000-0001-5950-6039 researchmap permalink toyodam toyodam Scopus Author ID 7201358693 7201358693 著者名 Toyoda, Michisato
キーワード等	Ambient sampling and ionization
	Docosahexaenoic acid–containing phospholipids
	Lipid localization
	Mass spectrometry imaging
	Mouse testis
	Spermatogenesis
内容	The version of record of this article, first published in Analytical and Bioanalytical Chemistry, is available online at Publisher’s website: https://doi.org/10.1007/s00216-024-05641-x.
抄録	Mass spectrometry imaging (MSI) is a promising analytical method to visualize the distribution of lipids in biological tissues. To clarify the relationship between cellular distribution and lipid types in a tissue, it is crucial to achieve both an improvement in ion detection sensitivity and a reduction in the ionization area. We report methods for improving the efficiency of ion transfer to a mass spectrometer and miniaturizing the extraction area of a sample for tapping-mode scanning probe electrospray ionization (t-SPESI), atmospheric pressure sampling, and ionization methods. To verify the efficacy of the new t-SPESI measurement system, MSI was performed on mouse testes with a pixel size of 5 µm. Lipid images of the testes from wild-type (WT) and lysophospholipid acyltransferase 3 (LPLAT3) knockout mice revealed the characteristic distribution of docosahexaenoic acid–containing phospholipids (DHA-PLs). A comparison of the ion images obtained by MSI and optical images of the same tissues stained with hematoxylin and eosin suggested that the distribution of DHA-PLs was significantly altered by spermatogenesis in the WT mouse testes.
出版者	Springer Science and Business Media Deutschland GmbH
収録物名	Analytical and Bioanalytical Chemistry
巻(号)	417(2)
ページ	275-286
刊行年月	2024-11-22
言語	英語
ハンドルURL	https://hdl.handle.net/11094/98817
PISSN	16182642
eISSN	16182650
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1007/s00216-024-05641-x
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Analytical and Bioanalytical Chemistry. 2024, 417(2), p. 275-286
DC.title	Improved ion detection sensitivity in mass spectrometry imaging using tapping-mode scanning probe electrospray ionization to visualize localized lipids in mouse testes
DC.creator	Otsuka, Yoichi
	Okada, Maki
	Hashidate-Yoshida, Tomomi
	Nagata, Katsuyuki
	Yamada, Makoto
	Goto, Motohito
	Sun, Mengze
	Shindou, Hideo
	Toyoda, Michisato
DC.publisher	Springer Science and Business Media Deutschland GmbH
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-11-22
DC.identifier	https://doi.org/10.1007/s00216-024-05641-x
DC.subject	Ambient sampling and ionization
	Docosahexaenoic acid–containing phospholipids
	Lipid localization
	Mass spectrometry imaging
	Mouse testis
	Spermatogenesis
DC.description	The version of record of this article, first published in Analytical and Bioanalytical Chemistry, is available online at Publisher’s website: https://doi.org/10.1007/s00216-024-05641-x.
DCTERMS.abstract	Mass spectrometry imaging (MSI) is a promising analytical method to visualize the distribution of lipids in biological tissues. To clarify the relationship between cellular distribution and lipid types in a tissue, it is crucial to achieve both an improvement in ion detection sensitivity and a reduction in the ionization area. We report methods for improving the efficiency of ion transfer to a mass spectrometer and miniaturizing the extraction area of a sample for tapping-mode scanning probe electrospray ionization (t-SPESI), atmospheric pressure sampling, and ionization methods. To verify the efficacy of the new t-SPESI measurement system, MSI was performed on mouse testes with a pixel size of 5 µm. Lipid images of the testes from wild-type (WT) and lysophospholipid acyltransferase 3 (LPLAT3) knockout mice revealed the characteristic distribution of docosahexaenoic acid–containing phospholipids (DHA-PLs). A comparison of the ion images obtained by MSI and optical images of the same tissues stained with hematoxylin and eosin suggested that the distribution of DHA-PLs was significantly altered by spermatogenesis in the WT mouse testes.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	98817
citation_title	Improved ion detection sensitivity in mass spectrometry imaging using tapping-mode scanning probe electrospray ionization to visualize localized lipids in mouse testes
citation_author	Otsuka, Yoichi
	Okada, Maki
	Hashidate-Yoshida, Tomomi
	Nagata, Katsuyuki
	Yamada, Makoto
	Goto, Motohito
	Sun, Mengze
	Shindou, Hideo
	Toyoda, Michisato
citation_publisher	Springer Science and Business Media Deutschland GmbH
citation_language	英語
citation_date	2024-11-22
citation_journal_title	Analytical and Bioanalytical Chemistry
citation_volume	417
citation_issue	2
citation_firstpage	275
citation_lastpage	286
citation_doi	https://doi.org/10.1007/s00216-024-05641-x
citation_public_url	https://hdl.handle.net/11094/98817

Improved ion detection sensitivity in mass spectrometry imaging using tapping-mode scanning probe electrospray ionization to visualize localized lipids in mouse testes

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