学術雑誌論文

学術雑誌論文

Oncotarget. 2018, 9(75), p. 34132-34141

Establishment of a novel platform cell line for efficient and precise evaluation of T cell receptor functional avidity

Impact Journals

英語

2018-09-25

https://doi.org/10.18632/oncotarget.26139

Morimoto S., Fujiki F., Kondo K., et al. Establishment of a novel platform cell line for efficient and precise evaluation of T cell receptor functional avidity. Oncotarget 9, 34132 (2018); https://doi.org/10.18632/oncotarget.26139.

Adoptive T-cell therapy with T cell receptor (TCR) -engineered T cells is an attractive strategy for cancer treatment and the success in this therapy is dependent on the functional avidity of the transduced TCRs against targeted tumor antigens. Therefore, the establishment of the methodology of the efficient and precise evaluation of TCR functional avidity has been awaited. Here, we show a novel platform cell line, named 2D3, which enables the functional avidity of transduced TCRs to be evaluated efficiently and precisely. In the 2D3, the precise TCR functional avidity of transduced TCRs is easily evaluable by the expression of green fluorescent protein (GFP) reporter gene driven by nuclear factor of activated T cells (NFAT) activation via TCR signaling. Four different TCRs of HLA-A*24:02-restricted Wilms' tumor gene 1 (WT1)-specific CD8+ cytotoxic T lymphocytes (CTLs) were transduced into 2D3 cells and the functional avidities of these four TCRs were evaluated. The evaluated functional avidity of these TCRs positively correlated with cell proliferation, cytokine production, and WT1-specific cytotoxicity of the TCR-transduced CD8+ T cells in response to WT1 antigen. These results showed that 2D3 cell line was a novel and stable tool useful for the efficient and precise evaluation of the functional avidity of isolated and transduced TCRs in developing TCR-based immunotherapy.

© Morimoto et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

98828

Establishment of a novel platform cell line for efficient and precise evaluation of T cell receptor functional avidity

Impact Journals

英語

2018-09-25

Oncotarget

9

75

34132

34141

https://doi.org/10.18632/oncotarget.26139

https://hdl.handle.net/11094/98828