Light-Activated Gene Expression System Using a Caging-Group-Free Photoactivatable Dye

Nonomura, Tatsuki; Minoshima, Masafumi; Kikuchi, Kazuya

Angewandte Chemie - International Edition, 2024, 64(4), e202416420

アクセス数:351件（2025-08-15 08:02 集計）

固定URL: https://hdl.handle.net/11094/98886

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タイトル
Light-Activated Gene Expression System Using a Caging-Group-Free Photoactivatable Dye
著者
著者名
Nonomura, Tatsuki
NRID
1000020600844 1000020600844
ORCID
0000-0002-9352-3454 0000-0002-9352-3454
researchmap permalink
cbatgcm_m33 cbatgcm_m33
Scopus Author ID
12774750700 12774750700
著者名
Minoshima, Masafumi
NRID
1000070292951 1000070292951
researchmap permalink
kikuchilab315 kikuchilab315
Scopus Author ID
7404088901 7404088901
著者名
Kikuchi, Kazuya
キーワード等
cell-free transcription
multidrug-binding transcriptional regulator
optical gene expression
photoactivatable dye
transcriptional regulation
内容
Nonomura T., Minoshima M., Kikuchi K. Light-Activated Gene Expression System Using a Caging-Group-Free Photoactivatable Dye. Angewandte Chemie - International Edition, (2024); https://doi.org/10.1002/anie.202416420.
抄録
Optical regulation of transcription using chemical compounds is an effective strategy to manipulate gene expression spatiotemporally. Conventional caging approaches with photoremovable protecting groups may require intense UV-light exposure and release potentially toxic byproducts. To address these problems, here we developed a light-mediated transcriptional regulation system by combining a caging-group-free photoactivatable dye PaX560 and a multidrug-binding transcriptional regulator QacR. The cationic dye generated from PaX560 through traceless photoconversion bound QacR and reduced its repressor function, resulting in transcriptional activation. Importantly, this system allowed transcriptional activation with a large dynamic range under mild visible light exposure and simultaneous detection of the state of the photoactivated effector. This module was integrated into the T7 RNA polymerase expression system to demonstrate light-activated transcription in vitro and in living cells.
出版者
John Wiley and Sons Inc
収録物名
Angewandte Chemie - International Edition
巻(号)
64(4)
ページ
e202416420
刊行年月
2024-11-06
言語
英語
ハンドルURL
https://hdl.handle.net/11094/98886
PISSN
14337851
eISSN
15213773
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1002/anie.202416420
アクセス権
open access
権利情報
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article