New multilocus sequence typing scheme for Enterococcus faecium reveals sequential outbreaks of vancomycin-resistant E. faecium ST1162 and ST610 in a Japanese tertiary medical center

Karino, Masaki; Yanagihara, Masashi; Harada, Tetsuya; Sugo, Megumi; Karino, Mizuki; Ohtaki, Hirofumi; Hanada, Hiroyuki; Takano, Toru; Yamato, Masaya; Okamoto, Shigefumi

doi:https://doi.org/10.1128/spectrum.02131-24

New multilocus sequence typing scheme for Enterococcus faecium reveals sequential outbreaks of vancomycin-resistant E. faecium ST1162 and ST610 in a Japanese tertiary medical center

Karino, Masaki; Yanagihara, Masashi; Harada, Tetsuya et al.

Microbiology Spectrum, 2025, 13(1), e02131-24

アクセス数:698件（2025-08-30 07:13 集計）

固定URL: https://hdl.handle.net/11094/99611

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タイトル	New multilocus sequence typing scheme for Enterococcus faecium reveals sequential outbreaks of vancomycin-resistant E. faecium ST1162 and ST610 in a Japanese tertiary medical center
著者	著者名 Karino, Masaki
	NRID 1000040379954 1000040379954 researchmap permalink 7000022349 7000022349 著者名 Yanagihara, Masashi
	NRID 1000070516723 1000070516723 著者名 Harada, Tetsuya
	著者名 Sugo, Megumi
	著者名 Karino, Mizuki
	NRID 1000000733172 1000000733172 著者名 Ohtaki, Hirofumi
	著者名 Hanada, Hiroyuki
	NRID 1000000263236 1000000263236 researchmap permalink read0185101 read0185101 著者名 Takano, Toru
	著者名 Yamato, Masaya
	NRID 1000050311759 1000050311759 ORCID 0000-0001-9359-380X 0000-0001-9359-380X researchmap permalink SOkamoto_20200212 SOkamoto_20200212 著者名 Okamoto, Shigefumi
キーワード等	Enterococcus faecium
	vancomycin-resistant
	multilocus sequence typing
	pulsed-field gel electrophoresis
	outbreak
内容	Copyright © American Society for Microbiology, Microbiology Spectrum 13(1), 2025, e02131-24; https://doi.org/10.1128/spectrum.02131-24.
抄録	Vancomycin-resistant Enterococcus faecium (VREfm) is a major nosocomial pathogen, and molecular epidemiological tools are crucial for controlling its spread. Pulsed-field gel electrophoresis (PFGE) is still used in clinical laboratories despite the increased accessibility of whole-genome sequencing (WGS). As PFGE equipment is no longer commercially available, clinical laboratories need alternative tools. Highly standardized multilocus sequence typing (MLST) is one option. However, the original MLST scheme for E. faecium, designed in 2002, showed inconsistencies with WGS-based typing. Therefore, the new Bezdíček MLST scheme, which offers more accurate genetic similarity based on genome-wide data, has recently been proposed. To clarify its clinical utility in analyzing nosocomial VREfm transmission, we compared both MLST schemes with PFGE using 68 VREfm isolates collected during an outbreak at a Japanese tertiary medical center in 2019. PFGE analysis identified nine clusters among the 68 strains, including two predominant clusters. The original scheme identified five sequence types (STOs), of which 82.4% (56/68) were STO192. The Bezdíček scheme identified nine sequence types (STBs), subdividing the original STO192 into STB1162 (30/56), STB610 (25/56), and STB895 (1/56). Simpson’s index of diversity values were 0.635, 0.317, and 0.648 for PFGE, the original scheme, and the Bezdíček scheme, respectively. Combining the Bezdíček scheme with admission records provided clearer outbreak visualization, indicating that two distinct STBs independently caused sequential outbreaks. With high discriminatory power comparable with PFGE and global availability, the Bezdíček scheme is a practical and valuable tool for controlling nosocomial VREfm infections in clinical laboratories.
出版者	American Society for Microbiology
収録物名	Microbiology Spectrum
巻(号)	13(1)
ページ	e02131-24
刊行年月	2025-01-07
言語	英語
ハンドルURL	https://hdl.handle.net/11094/99611
eISSN	21650497
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1128/spectrum.02131-24
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCTERMS.bibliographicCitation	Microbiology Spectrum. 2025, 13(1), p. e02131-24
DC.title	New multilocus sequence typing scheme for Enterococcus faecium reveals sequential outbreaks of vancomycin-resistant E. faecium ST1162 and ST610 in a Japanese tertiary medical center
DC.creator	Karino, Masaki
	Yanagihara, Masashi
	Harada, Tetsuya
	Sugo, Megumi
	Karino, Mizuki
	Ohtaki, Hirofumi
	Hanada, Hiroyuki
	Takano, Toru
	Yamato, Masaya
	Okamoto, Shigefumi
DC.publisher	American Society for Microbiology
DC.language' scheme='DCTERMS.RFC1766	英語
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-01-07
DC.identifier	https://doi.org/10.1128/spectrum.02131-24
DC.subject	Enterococcus faecium
	vancomycin-resistant
	multilocus sequence typing
	pulsed-field gel electrophoresis
	outbreak
DC.description	Copyright © American Society for Microbiology, Microbiology Spectrum 13(1), 2025, e02131-24; https://doi.org/10.1128/spectrum.02131-24.
DCTERMS.abstract	Vancomycin-resistant Enterococcus faecium (VREfm) is a major nosocomial pathogen, and molecular epidemiological tools are crucial for controlling its spread. Pulsed-field gel electrophoresis (PFGE) is still used in clinical laboratories despite the increased accessibility of whole-genome sequencing (WGS). As PFGE equipment is no longer commercially available, clinical laboratories need alternative tools. Highly standardized multilocus sequence typing (MLST) is one option. However, the original MLST scheme for E. faecium, designed in 2002, showed inconsistencies with WGS-based typing. Therefore, the new Bezdíček MLST scheme, which offers more accurate genetic similarity based on genome-wide data, has recently been proposed. To clarify its clinical utility in analyzing nosocomial VREfm transmission, we compared both MLST schemes with PFGE using 68 VREfm isolates collected during an outbreak at a Japanese tertiary medical center in 2019. PFGE analysis identified nine clusters among the 68 strains, including two predominant clusters. The original scheme identified five sequence types (STOs), of which 82.4% (56/68) were STO192. The Bezdíček scheme identified nine sequence types (STBs), subdividing the original STO192 into STB1162 (30/56), STB610 (25/56), and STB895 (1/56). Simpson’s index of diversity values were 0.635, 0.317, and 0.648 for PFGE, the original scheme, and the Bezdíček scheme, respectively. Combining the Bezdíček scheme with admission records provided clearer outbreak visualization, indicating that two distinct STBs independently caused sequential outbreaks. With high discriminatory power comparable with PFGE and global availability, the Bezdíček scheme is a practical and valuable tool for controlling nosocomial VREfm infections in clinical laboratories.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	99611
citation_title	New multilocus sequence typing scheme for Enterococcus faecium reveals sequential outbreaks of vancomycin-resistant E. faecium ST1162 and ST610 in a Japanese tertiary medical center
citation_author	Karino, Masaki
	Yanagihara, Masashi
	Harada, Tetsuya
	Sugo, Megumi
	Karino, Mizuki
	Ohtaki, Hirofumi
	Hanada, Hiroyuki
	Takano, Toru
	Yamato, Masaya
	Okamoto, Shigefumi
citation_publisher	American Society for Microbiology
citation_language	英語
citation_date	2025-01-07
citation_journal_title	Microbiology Spectrum
citation_volume	13
citation_issue	1
citation_firstpage	e02131-24
citation_doi	https://doi.org/10.1128/spectrum.02131-24
citation_public_url	https://hdl.handle.net/11094/99611

New multilocus sequence typing scheme for Enterococcus faecium reveals sequential outbreaks of vancomycin-resistant E. faecium ST1162 and ST610 in a Japanese tertiary medical center

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