Human induced pluripotent stem cell-derived cardiomyocyte patches ameliorate right ventricular function in a rat pressure-overloaded right ventricle model

Watanabe, Takuji; Kawamura, Takuji; Harada, Akima; Taira, Masaki; Yoshioka, Daisuke; Shimamura, Kazuo; Watabe, Tadashi; Shimosegawa, Eku; Ueno, Takayoshi; Miyagawa, Shigeru

doi:https://doi.org/10.1007/s10047-024-01479-3

Human induced pluripotent stem cell-derived cardiomyocyte patches ameliorate right ventricular function in a rat pressure-overloaded right ventricle model

Watanabe, Takuji; Kawamura, Takuji; Harada, Akima et al.

Journal of Artificial Organs, 2024

Number of Access:1,652（2025-07-28 20:12 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/100124

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Title	Human induced pluripotent stem cell-derived cardiomyocyte patches ameliorate right ventricular function in a rat pressure-overloaded right ventricle model
Creator	NRID 1000080838582 1000080838582 researchmap permalink takuji-watanabe takuji-watanabe Scopus Author ID 57209196810 57209196810 CreatorName Watanabe, Takuji
	NRID 1000060839398 1000060839398 researchmap permalink takuji-kawamura takuji-kawamura Scopus Author ID 55331768500 55331768500 ORCID 0000-0003-2985-0036 0000-0003-2985-0036 CreatorName Kawamura, Takuji
	NRID 1000070535426 1000070535426 CreatorName Harada, Akima
	NRID 1000090624651 1000090624651 Scopus Author ID 57188551084 57188551084 CreatorName Taira, Masaki
	NRID 1000040645959 1000040645959 researchmap permalink d-yoshioka d-yoshioka Scopus Author ID 7003892845 7003892845 CreatorName Yoshioka, Daisuke
	NRID 1000010507205 1000010507205 researchmap permalink kazuo-shimamura kazuo-shimamura Scopus Author ID 35969640700 35969640700 CreatorName Shimamura, Kazuo
	NRID 1000090648932 1000090648932 ORCID 0000-0001-8658-2395 0000-0001-8658-2395 researchmap permalink twosaka2018 twosaka2018 Scopus Author ID 57421862900 57421862900 CreatorName Watabe, Tadashi
	NRID 1000030370258 1000030370258 CreatorName Shimosegawa, Eku
	NRID 1000060437316 1000060437316 researchmap permalink takayoshi-ueno takayoshi-ueno Scopus Author ID 8137931400 8137931400 CreatorName Ueno, Takayoshi
	NRID 1000070544237 1000070544237 ORCID 0000-0003-0015-6569 0000-0003-0015-6569 researchmap permalink s-miyagawa3154 s-miyagawa3154 Scopus Author ID 55560624700 55560624700 CreatorName Miyagawa, Shigeru
Subject	Cardiomyocyte patch
	Congenital heart disease
	hiPS-CM transplantation
	Pressure-overloaded RV failure
	RV failure
Description	The version of record of this article, first published in Journal of Artificial Organs, is available online at Publisher’s website: https://doi.org/10.1007/s10047-024-01479-3.
Abstract	Right ventricular (RV) failure following surgical repair of congenital heart disease affects survival. Human induced pluripotent stem cell-derived cardiomyocyte (hiPS-CM) sheet transplantation ameliorated left ventricular dysfunction in preclinical studies, indicating its efficacy in RV failure in congenital heart disease. This study aimed to evaluate whether hiPS-CMs could improve RV function in rats with pressure-overloaded RV failure. F344/NJcl-rnu/rnu rats underwent pulmonary artery banding (PAB) via left thoracotomy. Four weeks after PAB, hiPS-CM patch transplantation to the RV was performed in the hiPS-CM group (n = 33), and a sham operation was performed in the sham group (n = 18). We evaluated cardiac catheterization, positron emission tomography data, and pathological results 8 weeks following PAB. RV end-diastolic pressure, the time constant of isovolumic relaxation, and end-diastolic pressure–volume relation were significantly ameliorated in the hiPS-CM group compared with in the sham group. Picrosirius red staining revealed that anti-fibrotic effects were significantly higher in the hiPS-CM group than in the sham group. Von Willebrand factor staining revealed significantly higher myocardial capillary vascular density in the hiPS-CM group than in the sham group. hiPS-CMs were detected in the epicardium 4 weeks after hiPS-CM sheet transplantation. The angiogenic gene expression in the myocardium was significantly higher in the hiPS-CM group than in the sham group. Overall, in rats with pressure-overloaded RV failure, hiPS-CM patch transplantation could improve diastolic function, suppress ventricular fibrosis, and increase capillary density, suggesting that it is a promising treatment for RV failure.
Publisher	Springer
Source Title	Journal of Artificial Organs
Date of Issued	2024-12-06
Language	English
Handle URL	https://hdl.handle.net/11094/100124
PISSN	14347229
eISSN	16190904
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1007/s10047-024-01479-3
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Journal of Artificial Organs. 2024
DC.title	Human induced pluripotent stem cell-derived cardiomyocyte patches ameliorate right ventricular function in a rat pressure-overloaded right ventricle model
DC.creator	Watanabe, Takuji
	Kawamura, Takuji
	Harada, Akima
	Taira, Masaki
	Yoshioka, Daisuke
	Shimamura, Kazuo
	Watabe, Tadashi
	Shimosegawa, Eku
	Ueno, Takayoshi
	Miyagawa, Shigeru
DC.publisher	Springer
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-12-06
DC.identifier	https://doi.org/10.1007/s10047-024-01479-3
DC.subject	Cardiomyocyte patch
	Congenital heart disease
	hiPS-CM transplantation
	Pressure-overloaded RV failure
	RV failure
DC.description	The version of record of this article, first published in Journal of Artificial Organs, is available online at Publisher’s website: https://doi.org/10.1007/s10047-024-01479-3.
DCTERMS.abstract	Right ventricular (RV) failure following surgical repair of congenital heart disease affects survival. Human induced pluripotent stem cell-derived cardiomyocyte (hiPS-CM) sheet transplantation ameliorated left ventricular dysfunction in preclinical studies, indicating its efficacy in RV failure in congenital heart disease. This study aimed to evaluate whether hiPS-CMs could improve RV function in rats with pressure-overloaded RV failure. F344/NJcl-rnu/rnu rats underwent pulmonary artery banding (PAB) via left thoracotomy. Four weeks after PAB, hiPS-CM patch transplantation to the RV was performed in the hiPS-CM group (n = 33), and a sham operation was performed in the sham group (n = 18). We evaluated cardiac catheterization, positron emission tomography data, and pathological results 8 weeks following PAB. RV end-diastolic pressure, the time constant of isovolumic relaxation, and end-diastolic pressure–volume relation were significantly ameliorated in the hiPS-CM group compared with in the sham group. Picrosirius red staining revealed that anti-fibrotic effects were significantly higher in the hiPS-CM group than in the sham group. Von Willebrand factor staining revealed significantly higher myocardial capillary vascular density in the hiPS-CM group than in the sham group. hiPS-CMs were detected in the epicardium 4 weeks after hiPS-CM sheet transplantation. The angiogenic gene expression in the myocardium was significantly higher in the hiPS-CM group than in the sham group. Overall, in rats with pressure-overloaded RV failure, hiPS-CM patch transplantation could improve diastolic function, suppress ventricular fibrosis, and increase capillary density, suggesting that it is a promising treatment for RV failure.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	100124
citation_title	Human induced pluripotent stem cell-derived cardiomyocyte patches ameliorate right ventricular function in a rat pressure-overloaded right ventricle model
citation_author	Watanabe, Takuji
	Kawamura, Takuji
	Harada, Akima
	Taira, Masaki
	Yoshioka, Daisuke
	Shimamura, Kazuo
	Watabe, Tadashi
	Shimosegawa, Eku
	Ueno, Takayoshi
	Miyagawa, Shigeru
citation_publisher	Springer
citation_language	English
citation_date	2024-12-06
citation_journal_title	Journal of Artificial Organs
citation_doi	https://doi.org/10.1007/s10047-024-01479-3
citation_public_url	https://hdl.handle.net/11094/100124

Human induced pluripotent stem cell-derived cardiomyocyte patches ameliorate right ventricular function in a rat pressure-overloaded right ventricle model

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