Toxoplasma GRA15 expression on dendritic cells inhibits B cell differentiation and antibody production

Nakayama, Yuki; Ihara, Fumiaki; Okuzaki, Daisuke et al.

Parasitology International, 2025, 105, 102995

Number of Access:910（2025-08-18 12:33 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/100208

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Title
Toxoplasma GRA15 expression on dendritic cells inhibits B cell differentiation and antibody production
Creator
CreatorName
Nakayama, Yuki
NRID
1000000800773 1000000800773
researchmap permalink
fihara fihara
CreatorName
Ihara, Fumiaki
NRID
1000000346131 1000000346131
ORCID
0000-0002-4552-783X 0000-0002-4552-783X
researchmap permalink
read0092307 read0092307
Scopus Author ID
6506703054 6506703054
CreatorName
Okuzaki, Daisuke
NRID
1000090431395 1000090431395
CreatorName
Nishikawa, Yoshifumi
NRID
1000030631551 1000030631551
ORCID
0000-0001-6440-3958 0000-0001-6440-3958
researchmap permalink
miwa-s miwa-s
Scopus Author ID
16319941500 16319941500
CreatorName
Sasai, Miwa
NRID
1000000444521 1000000444521
researchmap permalink
read0151116 read0151116
Scopus Author ID
55552856200 55552856200
CreatorName
Yamamoto, Masahiro
Subject
B cell
GRA15
Plasma cell and germinal center B cell
Specific antibody production
Toxoplasma gondii
Description
Nakayama Y., Ihara F., Okuzaki D., et al. Toxoplasma GRA15 expression on dendritic cells inhibits B cell differentiation and antibody production. Parasitology International 105, 102995 (2025); https://doi.org/10.1016/j.parint.2024.102995.
Abstract
One of the dense granule proteins named GRA15 in Toxoplasma gondii (T. gondii), is known to support an innate immune response in host through activation of NF-κB. However, little is known about advantages of GRA15 for parasites. By examining the role of GRA15 in the host-parasite interactions, it was clarified that GRA15 in T. gondii suppressed acquired immune responses in host. Wild-type parasite infection to C57BL/6 mice resulted in lower titers of T. gondii antibody and lower plasma cell counts compared to Δgra15 T. gondii. To identify host cells in which GRA15 acts to suppress antibody production, we generated conditional knock-in mice that express GRA15 in specific cell lineages. Anti-T. gondii antibodies were not reduced in macrophages of conditional knock-in mice after infection with Δgra15 T. gondii, while the production of T. gondii antibody was suppressed in dendritic cells of the conditional knock-in mice (CD11c-Cre/GRA15cKI). In the CD11c-Cre/GRA15cKI immunized with ovalbumin (OVA), the titers of anti-OVA antibody were reduced compared to control mice. Furthermore, the number of OVA antigen-specific T cells was also decreased in CD11c-Cre/GRA15cKI. These data showed that GRA15 in dendritic cells suppressed T cell-mediated humoral immunity. These findings might implicate the pathological significance of GRA15 and facilitate Toxoplasma vaccines production.
Publisher
Elsevier Ireland Ltd
Source Title
Parasitology International
Volume (Issue)
105
Page
102995
Date of Issued
2025-04-01
Language
English
Handle URL
https://hdl.handle.net/11094/100208
PISSN
13835769
eISSN
18730329
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1016/j.parint.2024.102995
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article