Recent advances in identifying and characterizing secretory proteins of Toxoplasma gondii by CRISPR-based screening

Tachibana, Yuta; Yamamoto, Masahiro

Parasitology International, 2025, 105, 102997

Number of Access:200（2025-08-18 12:33 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/100210

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Title
Recent advances in identifying and characterizing secretory proteins of Toxoplasma gondii by CRISPR-based screening
Creator
CreatorName
Tachibana, Yuta
NRID
1000000444521 1000000444521
researchmap permalink
read0151116 read0151116
Scopus Author ID
55552856200 55552856200
CreatorName
Yamamoto, Masahiro
Subject
CRISPR screen
Dense granule
Microneme
Rhoptry
Secretome
Secretory proteins
Toxoplasma gondii
Description
Tachibana Y., Yamamoto M. Recent advances in identifying and characterizing secretory proteins of Toxoplasma gondii by CRISPR-based screening. Parasitology International 105, 102997 (2025); https://doi.org/10.1016/j.parint.2024.102997.
Abstract
The apicomplexan parasite, Toxoplasma gondii, develops unique secretory organelles, such as micronemes, rhoptries, and dense granules, which do not exist in other well-studied eukaryotic organisms. These secretory organelles are key features of apicomplexan parasites and discharge various proteins that are essential for invasion, replication, egress, host-parasite interactions, and virulence. Many studies have therefore focused on identifying and characterizing the proteins secreted by T. gondii that play essential roles in pathology and that can be targeted for therapeutics and vaccine development. The recent development of functional genetic screens based on CRISPR/Cas9 technology has revolutionized this field and has enabled the identification of genes that contribute to parasite fitness in vitro and in vivo. Consequently, characterization of genes identified by unbiased CRISPR screens has revealed novel aspects of apicomplexan biology. In this review, we describe the development of CRIPSR-based screening technology for T. gondii, and recent advances in our understanding of secretory proteins identified and characterized by CRISPR-based screening.
Publisher
Elsevier Ireland Ltd
Source Title
Parasitology International
Volume (Issue)
105
Page
102997
Date of Issued
2025-04-01
Language
English
Handle URL
https://hdl.handle.net/11094/100210
PISSN
13835769
eISSN
18730329
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1016/j.parint.2024.102997
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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Version of Record
Category
学術雑誌論文 Journal Article