Genetically encoded bioluminescent glucose indicator for biological research

Tanaka, Rikuto; Sugiura, Kazunori; Osabe, Kenji; Hattori, Mitsuru; Nagai, Takeharu

doi:https://doi.org/10.1016/j.bbrc.2024.151092

Genetically encoded bioluminescent glucose indicator for biological research

Tanaka, Rikuto; Sugiura, Kazunori; Osabe, Kenji et al.

Biochemical and Biophysical Research Communications, 2025, 742, 151092

Number of Access:653（2025-11-03 13:23 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/100214

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Title	Genetically encoded bioluminescent glucose indicator for biological research
Creator	CreatorName Tanaka, Rikuto
	CreatorName Sugiura, Kazunori
	NRID 1000060751352 1000060751352 CreatorName Osabe, Kenji
	NRID 1000020589858 1000020589858 researchmap permalink mhatto mhatto Scopus Author ID 7401882679 7401882679 ORCID 0000-0003-1189-6554 0000-0003-1189-6554 CreatorName Hattori, Mitsuru
	NRID 1000020311350 1000020311350 ORCID 0000-0003-2650-9895 0000-0003-2650-9895 researchmap permalink ng1 ng1 Scopus Author ID 7403433377 7403433377 CreatorName Nagai, Takeharu
Subject	Bioluminescence imaging
	Genetically encoded bioluminescent indicator
	Glucose metabolism
Description	Tanaka R., Sugiura K., Osabe K., et al. Genetically encoded bioluminescent glucose indicator for biological research. Biochemical and Biophysical Research Communications 742, 151092 (2025); https://doi.org/10.1016/j.bbrc.2024.151092.
Abstract	Glucose is an essential energy source in living cells and is involved in various phenomena. To understand the roles of glucose, measuring cellular glucose levels is important. Here, we developed a bioluminescent glucose indicator called LOTUS-Glc. Unlike fluorescence, bioluminescence doesn't require excitation light when imaging. Using LOTUS-Glc, we demonstrated drug effect evaluation, concurrent use with the optogenetic tool in HEK293T cells, and the measurement of light-dependent glucose fluctuations in plant-derived protoplasts. LOTUS-Glc would be a useful tool for understanding the roles of glucose in living organisms.
Publisher	Elsevier B.V.
Source Title	Biochemical and Biophysical Research Communications
Volume (Issue)	742
Page	151092
Date of Issued	2025-01-01
Language	English
Handle URL	https://hdl.handle.net/11094/100214
PISSN	0006291X
eISSN	10902104
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.bbrc.2024.151092
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Biochemical and Biophysical Research Communications. 2025, 742, p. 151092
DC.title	Genetically encoded bioluminescent glucose indicator for biological research
DC.creator	Tanaka, Rikuto
	Sugiura, Kazunori
	Osabe, Kenji
	Hattori, Mitsuru
	Nagai, Takeharu
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-01-01
DC.identifier	https://doi.org/10.1016/j.bbrc.2024.151092
DC.subject	Bioluminescence imaging
	Genetically encoded bioluminescent indicator
	Glucose metabolism
DC.description	Tanaka R., Sugiura K., Osabe K., et al. Genetically encoded bioluminescent glucose indicator for biological research. Biochemical and Biophysical Research Communications 742, 151092 (2025); https://doi.org/10.1016/j.bbrc.2024.151092.
DCTERMS.abstract	Glucose is an essential energy source in living cells and is involved in various phenomena. To understand the roles of glucose, measuring cellular glucose levels is important. Here, we developed a bioluminescent glucose indicator called LOTUS-Glc. Unlike fluorescence, bioluminescence doesn't require excitation light when imaging. Using LOTUS-Glc, we demonstrated drug effect evaluation, concurrent use with the optogenetic tool in HEK293T cells, and the measurement of light-dependent glucose fluctuations in plant-derived protoplasts. LOTUS-Glc would be a useful tool for understanding the roles of glucose in living organisms.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	100214
citation_title	Genetically encoded bioluminescent glucose indicator for biological research
citation_author	Tanaka, Rikuto
	Sugiura, Kazunori
	Osabe, Kenji
	Hattori, Mitsuru
	Nagai, Takeharu
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2025-01-01
citation_journal_title	Biochemical and Biophysical Research Communications
citation_volume	742
citation_firstpage	151092
citation_doi	https://doi.org/10.1016/j.bbrc.2024.151092
citation_public_url	https://hdl.handle.net/11094/100214

Genetically encoded bioluminescent glucose indicator for biological research

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