Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae

Ohe, Yutaro; Hasebe, Masaharu; Hamanaka, Yoshitaka et al.

Journal of Comparative Physiology A, 2025

Number of Access:293（2025-09-07 12:27 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/100330

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Title
Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae
Creator
CreatorName
Ohe, Yutaro
NRID
1000040802822 1000040802822
CreatorName
Hasebe, Masaharu
NRID
1000010647572 1000010647572
researchmap permalink
7_7_7 7_7_7
Scopus Author ID
36769083600 36769083600
CreatorName
Hamanaka, Yoshitaka
NRID
1000070347483 1000070347483
CreatorName
Goto, G. Shin
NRID
1000090254383 1000090254383
researchmap permalink
read0182838 read0182838
Scopus Author ID
7006284711 7006284711
CreatorName
Shiga, Sakiko
Subject
PDF
PTTH
s-LNv
Varicosity
Glutamate
sNPF
Description
The version of record of this article, first published in Journal of Comparative Physiology A, is available online at Publisher’s website: https://doi.org/10.1007/s00359-024-01729-y.
Abstract
Larvae of the flesh fly, Sarcophaga similis exhibit photoperiodic responses to control pupal diapause. Although the external coincidence model is applicable to S. similis photoperiodism, it remains unknown how the circadian clock system integrates day-length information. To explore the mechanisms, we examined the neural circuitry involving circadian clock lateral neurons (LNs) and prothoracicotropic hormone (PTTH) neurons. We also examined the photoperiodic effects on LN-fiber patterns in third-instar S. similis larvae. Immunohistochemistry showed that the clock protein PERIOD and the neuropeptide pigment-dispersing factor (PDF) were co-localized in four cells per brain hemisphere, and we named these PDF-LNs of S. similis. Single-cell polymerase chain reaction of backfilled neurons from the ring gland showed that two pairs of pars lateralis neurons with contralateral axons (PL-c neurons) to the ring gland expressed ptth. Double labeling with immunohistochemistry and backfills revealed that PDF-immunoreactive varicose fibers projected close to fibers from PL-c neurons. short neuropeptide f (snpf) receptor and glutamate-gated chloride channel but not pdf receptor were expressed in PL-c neurons. sNPF and L-glutamate but not PDF acutely inhibited the spontaneous firing activity of PL-c neurons. The number of PDF-immunoreactive varicosities of PDF-LNs in the dorsal protocerebrum was significantly higher under short-day than that under long-day conditions in a time-dependent manner. These results suggest that sNPF and/or glutamate signaling to PTTH neurons and PDF-LNs form a potential neural circuity for the photoperiodic control of pupal diapause and that photoperiod modifies the connectivity strength between PDF-LNs and their post- or pre-neurons in the circuitry.
Publisher
Springer Science and Business Media LLC
Source Title
Journal of Comparative Physiology A
Date of Issued
2025-01-15
Language
English
Handle URL
https://hdl.handle.net/11094/100330
PISSN
03407594
eISSN
14321351
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1007/s00359-024-01729-y
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article