Enhanced Discriminability of Viral Vectors in Viscous Nanopores

Tsutsui, Makusu; Tsunekawa, Yuji; Wada, Mikako et al.

Small Methods, 2025, 2401321

Number of Access:891（2025-09-04 09:48 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/100348

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Title
Enhanced Discriminability of Viral Vectors in Viscous Nanopores
Creator
NRID
1000050546596 1000050546596
ORCID
0000-0002-4552-1163 0000-0002-4552-1163
researchmap permalink
makusu makusu
Scopus Author ID
8978905800 8978905800
CreatorName
Tsutsui, Makusu
CreatorName
Tsunekawa, Yuji
NRID
1000050810090 1000050810090
CreatorName
Wada, Mikako
NRID
1000020781347 1000020781347
CreatorName
Arima, Akihide
CreatorName
Onodera, Azusa
CreatorName
Nishina, Masumi
CreatorName
Nagoya, Miho
NRID
1000030183916 1000030183916
CreatorName
Baba, Yoshinobu
NRID
1000020092546 1000020092546
researchmap permalink
read0014173 read0014173
CreatorName
Kawai, Tomoji
NRID
1000000326828 1000000326828
CreatorName
Okada, Takashi
Subject
electrochemistry
virus vector
gene therapy
nanofluidics
nanopore
Description
Tsutsui M., Tsunekawa Y., Wada M., et al. Enhanced Discriminability of Viral Vectors in Viscous Nanopores. Small Methods , (2025); https://doi.org/10.1002/smtd.202401321.
Abstract
Achieving safe and efficient gene therapy hinges upon the inspection of genomes enclosed within individual nano-carriers to mitigate potential health risks associated with empty or fragment-filled vectors. Here solid-state nanopore sensing is reported for identifications of intermediate adeno-associated virus (AAV) vectors in liquid. The method exploits the phenomenon of translocation slowdown induced by the viscosity of salt water-organic mixtures. This enables real-time ionic current measurements allowing precise tracking of the electroosmotic flow-driven motions of recombinant AAV vectors in a nanopore. The resulting ionic signals facilitate discrimination between replicative intermediates carrying ssDNA fragments and its full vector counterparts based on genome length-derived subtle nanometer differences in the viral diameters. This rapid and non-destructive means of genome analysis within virus capsids provides a promising avenue toward a robust methodology for ensuring the integrity of AAV vectors before administration.
Publisher
John Wiley and Sons Inc
Source Title
Small Methods
Page
2401321
Date of Issued
2025-01-02
Language
English
Handle URL
https://hdl.handle.net/11094/100348
eISSN
23669608
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1002/smtd.202401321
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article