Osteogenic tailoring of oriented bone matrix organization using on/off micropatterning for osteoblast adhesion on titanium surfaces

Matsuzaka, Tadaaki; Matsugaki, Aira; Ishihara, Kazuhiko; Nakano, Takayoshi

doi:https://doi.org/10.1016/j.actbio.2024.12.017

Osteogenic tailoring of oriented bone matrix organization using on/off micropatterning for osteoblast adhesion on titanium surfaces

Matsuzaka, Tadaaki; Matsugaki, Aira; Ishihara, Kazuhiko et al.

Acta Biomaterialia, 2025, 192, 487-500

Number of Access:372（2025-07-27 15:54 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/100380

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Title	Osteogenic tailoring of oriented bone matrix organization using on/off micropatterning for osteoblast adhesion on titanium surfaces
Creator	CreatorName Matsuzaka, Tadaaki
	NRID 1000010592529 1000010592529 ORCID 0000-0002-8427-7124 0000-0002-8427-7124 researchmap permalink aira aira Scopus Author ID 24172720900 24172720900 CreatorName Matsugaki, Aira
	NRID 1000090193341 1000090193341 researchmap permalink read0007946 read0007946 CreatorName Ishihara, Kazuhiko
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 CreatorName Nakano, Takayoshi
Subject	Apatite/collagen orientation
	Bone regeneration
	Osteoblast orientation
	Photoreactive MPC polymer
	Stripe-micropatterned titanium substrate
Description	Matsuzaka T., Matsugaki A., Ishihara K., et al. Osteogenic tailoring of oriented bone matrix organization using on/off micropatterning for osteoblast adhesion on titanium surfaces. Acta Biomaterialia 192, 487 (2025); https://doi.org/10.1016/j.actbio.2024.12.017.
Abstract	Titanium (Ti) implants are well known for their mechanical reliability and chemical stability, crucial for successful bone regeneration. Various shape control and surface modification techniques to enhance biological activity have been developed. Despite the crucial importance of the collagen/apatite bone microstructure for mechanical function, antimicrobial properties, and biocompatibility, precise and versatile pattern control for regenerating the microstructure remains challenging. Here, we developed a novel osteogenic tailoring stripe-micropatterned MPC-Ti substrate that induces genetic-level control of oriented bone matrix organization. This biomaterial was created by micropatterning 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer onto a titanium (Ti) surface through a selective photoreaction. The stripe-micropatterned MPC-Ti substrate establishes a distinct interface for cell adhesion, robustly inducing osteoblast cytoskeleton alignment through actin cytoskeletal alignment, and facilitating the formation of a bone-mimicking-oriented collagen/apatite tissue. Moreover, our study revealed that this bone alignment process is promoted through the activation of the Wnt/β-catenin signaling pathway, which is triggered by nuclear deformation induced by strong cellular alignment guidance. This innovative material is essential for personalized next-generation medical devices, offering high customizability and active restoration of the bone microstructure. Statement of Significance: This study demonstrates a novel osteogenic tailoring stripe-micropatterned MPC-Ti substrate that induces osteoblast alignment and bone matrix orientation based on genetic mechanism. By employing a light-reactive MPC polymer, we successfully micropatterned the titanium surface, creating a biomaterial that stimulates unidirectional osteoblast alignment and enhances the formation of natural bone-mimetic anisotropic microstructures. The innovative approach of regulating cell adhesion and cytoskeletal alignment activates the Wnt/β-catenin signaling pathway, crucial for both bone differentiation and orientation. This study presents the first biomaterial that artificially induces the construction of mechanically superior anisotropic bone tissue, and it is expected to promote functional bone regeneration by enhancing bone differentiation and orientation—targeting both the quantity and quality of bone tissue.
Publisher	Elsevier Ltd
Source Title	Acta Biomaterialia
Volume (Issue)	192
Page	487-500
Date of Issued	2025-01-15
Language	English
Handle URL	https://hdl.handle.net/11094/100380
PISSN	17427061
eISSN	18787568
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.actbio.2024.12.017
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	Acta Biomaterialia. 2025, 192, p. 487-500
DC.title	Osteogenic tailoring of oriented bone matrix organization using on/off micropatterning for osteoblast adhesion on titanium surfaces
DC.creator	Matsuzaka, Tadaaki
	Matsugaki, Aira
	Ishihara, Kazuhiko
	Nakano, Takayoshi
DC.publisher	Elsevier Ltd
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-01-15
DC.identifier	https://doi.org/10.1016/j.actbio.2024.12.017
DC.subject	Apatite/collagen orientation
	Bone regeneration
	Osteoblast orientation
	Photoreactive MPC polymer
	Stripe-micropatterned titanium substrate
DC.description	Matsuzaka T., Matsugaki A., Ishihara K., et al. Osteogenic tailoring of oriented bone matrix organization using on/off micropatterning for osteoblast adhesion on titanium surfaces. Acta Biomaterialia 192, 487 (2025); https://doi.org/10.1016/j.actbio.2024.12.017.
DCTERMS.abstract	Titanium (Ti) implants are well known for their mechanical reliability and chemical stability, crucial for successful bone regeneration. Various shape control and surface modification techniques to enhance biological activity have been developed. Despite the crucial importance of the collagen/apatite bone microstructure for mechanical function, antimicrobial properties, and biocompatibility, precise and versatile pattern control for regenerating the microstructure remains challenging. Here, we developed a novel osteogenic tailoring stripe-micropatterned MPC-Ti substrate that induces genetic-level control of oriented bone matrix organization. This biomaterial was created by micropatterning 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer onto a titanium (Ti) surface through a selective photoreaction. The stripe-micropatterned MPC-Ti substrate establishes a distinct interface for cell adhesion, robustly inducing osteoblast cytoskeleton alignment through actin cytoskeletal alignment, and facilitating the formation of a bone-mimicking-oriented collagen/apatite tissue. Moreover, our study revealed that this bone alignment process is promoted through the activation of the Wnt/β-catenin signaling pathway, which is triggered by nuclear deformation induced by strong cellular alignment guidance. This innovative material is essential for personalized next-generation medical devices, offering high customizability and active restoration of the bone microstructure. Statement of Significance: This study demonstrates a novel osteogenic tailoring stripe-micropatterned MPC-Ti substrate that induces osteoblast alignment and bone matrix orientation based on genetic mechanism. By employing a light-reactive MPC polymer, we successfully micropatterned the titanium surface, creating a biomaterial that stimulates unidirectional osteoblast alignment and enhances the formation of natural bone-mimetic anisotropic microstructures. The innovative approach of regulating cell adhesion and cytoskeletal alignment activates the Wnt/β-catenin signaling pathway, crucial for both bone differentiation and orientation. This study presents the first biomaterial that artificially induces the construction of mechanically superior anisotropic bone tissue, and it is expected to promote functional bone regeneration by enhancing bone differentiation and orientation—targeting both the quantity and quality of bone tissue.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	100380
citation_title	Osteogenic tailoring of oriented bone matrix organization using on/off micropatterning for osteoblast adhesion on titanium surfaces
citation_author	Matsuzaka, Tadaaki
	Matsugaki, Aira
	Ishihara, Kazuhiko
	Nakano, Takayoshi
citation_publisher	Elsevier Ltd
citation_language	English
citation_date	2025-01-15
citation_journal_title	Acta Biomaterialia
citation_volume	192
citation_firstpage	487
citation_lastpage	500
citation_doi	https://doi.org/10.1016/j.actbio.2024.12.017
citation_public_url	https://hdl.handle.net/11094/100380

Osteogenic tailoring of oriented bone matrix organization using on/off micropatterning for osteoblast adhesion on titanium surfaces

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