Creating coveted bioluminescence colors for simultaneous multi-color bioimaging

Hattori, Mitsuru; Wazawa, Tetsuichi; Orioka, Mariko et al.

Science Advances, 2025, 11(4), eadp4750

Number of Access:694（2025-11-02 16:26 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/100434

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Title
Creating coveted bioluminescence colors for simultaneous multi-color bioimaging
Creator
NRID
1000020589858 1000020589858
ORCID
0000-0003-1189-6554 0000-0003-1189-6554
researchmap permalink
mhatto mhatto
Scopus Author ID
7401882679 7401882679
CreatorName
Hattori, Mitsuru
NRID
1000080359851 1000080359851
researchmap permalink
wazawa wazawa
CreatorName
Wazawa, Tetsuichi
CreatorName
Orioka, Mariko
NRID
1000060710944 1000060710944
CreatorName
Hiruta, Yuki
NRID
1000020311350 1000020311350
ORCID
0000-0003-2650-9895 0000-0003-2650-9895
researchmap permalink
ng1 ng1
Scopus Author ID
7403433377 7403433377
CreatorName
Nagai, Takeharu
Description
Hattori M., Wazawa T., Orioka M., et al. Creating coveted bioluminescence colors for simultaneous multi-color bioimaging. Science Advances 11, eadp4750 (2025); https://doi.org/10.1126/sciadv.adp4750.
Abstract
Bioluminescence, an optical marker that does not require excitation by light, allows researchers to simultaneously observe multiple targets, each exhibiting a different color. Notably, the colors of the bioluminescent proteins must sufficiently vary to enable simultaneous detection. Here, we aimed to introduce a method that can be used to expand the color variation by tuning dual-acceptor bioluminescence resonance energy transfer. Using this approach, we could visualize multiple targets with up to 20 colors through single-shot acquisition using a color complementary metal-oxide semiconductor camera. Overall, this method enables simple and simultaneous observation of multiple biological targets and phenomena.
Publisher
American Association for the Advancement of Science
Source Title
Science Advances
Volume (Issue)
11(4)
Page
eadp4750
Date of Issued
2025-01-24
Language
English
Handle URL
https://hdl.handle.net/11094/100434
eISSN
23752548
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1126/sciadv.adp4750
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article