Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells

Kaneda, Eri; Kawai, Takafumi; Okamura, Yasushi et al.

Physiological Reports, 2025, 13(6), e70236

Number of Access:567（2025-07-18 10:04 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/100966

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Title
Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells
Creator
CreatorName
Kaneda, Eri
NRID
1000070614915 1000070614915
researchmap permalink
t_kawa t_kawa
Scopus Author ID
55465095900 55465095900
CreatorName
Kawai, Takafumi
NRID
1000080201987 1000080201987
ORCID
0000-0001-5386-7968 0000-0001-5386-7968
researchmap permalink
yasushiokamura yasushiokamura
Scopus Author ID
55322083200 55322083200
CreatorName
Okamura, Yasushi
NRID
1000070544237 1000070544237
ORCID
0000-0003-0015-6569 0000-0003-0015-6569
researchmap permalink
s-miyagawa3154 s-miyagawa3154
Scopus Author ID
55560624700 55560624700
CreatorName
Miyagawa, Shigeru
Subject
neuromodulation
patch clamp
static magnetic fields
sympathetic neuron
Description
Kaneda E., Kawai T., Okamura Y., et al. Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells. Physiological Reports 13, e70236 (2025); https://doi.org/10.14814/phy2.70236.
Abstract
While exposure of moderate static magnetic fields (SMF) can alter neuronal excitability, the effects on sympathetic neurons remain underexplored. This study investigates the effects of moderate SMF on Kv channels in the plasma membrane of sympathetic neuron-like PC12 cells. The current density of Kv channels was significantly lower in the 18-h magnet-exposed group, with effects persisting even after the magnet was removed before patch-clamp measurements. The current density of outward current in the presence of TEA was not different between the two groups, indicating that magnetic field affects TEA-sensitive Kv channels. To further explore these changes, RNA sequencing was performed on samples from both the Sham and 18-h magnet-exposed groups, identifying 37 moderate SMF-sensitive genes. Changes in mRNA expression levels and KEGG analysis suggested that pathways involved in the inhibition of neuronal excitability, such as GABAB receptor activation and Kir3 channel opening, may be more likely to be activated. In conclusion, moderate SMF is strongly associated with reduced current density in PC12 cells, particularly affecting Kv channels. The present study provides fundamental information on the influence of long-term SMF exposure on the excitability of sympathetic neurons.
Publisher
American Physiological Society
Source Title
Physiological Reports
Volume (Issue)
13(6)
Page
e70236
Date of Issued
2025-03-22
Language
English
Handle URL
https://hdl.handle.net/11094/100966
eISSN
2051817X
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.14814/phy2.70236
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article