Enhancing the efficacy of near-infrared photoimmunotherapy through intratumoural delivery of CD44–targeting antibody–photoabsorber conjugates

Adachi, Yuichi; Miyake, Kotaro; Ohira, Kika; Satoh, Shingo; Masuhiro, Kentaro; Edahiro, Ryuya; Shirai, Yuya; Naito, Maiko; Naito, Yujiro; Shiroyama, Takayuki; Koyama, Shohei; Hirata, Haruhiko; Iwahori, Kota; Nagatomo, Izumi; Takeda, Yoshito; Kumanogoh, Atsushi

doi:https://doi.org/10.1016/j.ebiom.2025.105566

Enhancing the efficacy of near-infrared photoimmunotherapy through intratumoural delivery of CD44–targeting antibody–photoabsorber conjugates

Adachi, Yuichi; Miyake, Kotaro; Ohira, Kika et al.

eBioMedicine, 2025, 112, 105566

Number of Access:885（2025-06-26 14:01 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/100994

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Title	Enhancing the efficacy of near-infrared photoimmunotherapy through intratumoural delivery of CD44–targeting antibody–photoabsorber conjugates
Creator	CreatorName Adachi, Yuichi
	NRID 1000080726787 1000080726787 researchmap permalink kotaro_researchmap kotaro_researchmap Scopus Author ID 55856984400 55856984400 CreatorName Miyake, Kotaro
	CreatorName Ohira, Kika
	CreatorName Satoh, Shingo
	CreatorName Masuhiro, Kentaro
	researchmap permalink ryuya_edahiro_88 ryuya_edahiro_88 CreatorName Edahiro, Ryuya
	NRID 1000050899945 1000050899945 CreatorName Shirai, Yuya
	NRID 1000010974686 1000010974686 researchmap permalink maikonaito maikonaito CreatorName Naito, Maiko
	NRID 1000030800982 1000030800982 researchmap permalink y.naito y.naito Scopus Author ID 57195252120 57195252120 CreatorName Naito, Yujiro
	NRID 1000060860062 1000060860062 researchmap permalink shiroyama_t shiroyama_t Scopus Author ID 36476578400 36476578400 CreatorName Shiroyama, Takayuki
	NRID 1000080767559 1000080767559 ORCID 0000-0002-6897-9417 0000-0002-6897-9417 researchmap permalink sk500 sk500 CreatorName Koyama, Shohei
	NRID 1000030546867 1000030546867 researchmap permalink hh_resmed hh_resmed Scopus Author ID 7202342767 7202342767 CreatorName Hirata, Haruhiko
	NRID 1000080566448 1000080566448 researchmap permalink 638ik 638ik Scopus Author ID 15738420000 15738420000 CreatorName Iwahori, Kota
	NRID 1000010570583 1000010570583 researchmap permalink saxon-w saxon-w Scopus Author ID 13404305500 13404305500 CreatorName Nagatomo, Izumi
	NRID 1000040452388 1000040452388 researchmap permalink yoshi10 yoshi10 Scopus Author ID 7404001848 7404001848 CreatorName Takeda, Yoshito
	NRID 1000010294125 1000010294125 ORCID 0000-0003-4749-7117 0000-0003-4749-7117 researchmap permalink read0051725 read0051725 Scopus Author ID 6602726311 6602726311 CreatorName Kumanogoh, Atsushi
Subject	Antibody–photoabsorber conjugate
	Intratumoural administration
	Lung cancer
	Photoimmunotherapy
Description	Adachi Y., Miyake K., Ohira K., et al. Enhancing the efficacy of near-infrared photoimmunotherapy through intratumoural delivery of CD44–targeting antibody–photoabsorber conjugates. eBioMedicine 112, 105566 (2025); https://doi.org/10.1016/j.ebiom.2025.105566.
Abstract	Background: Photoimmunotherapy (PIT) is a potent modality for cancer treatment. The conventional PIT regimen involves the systemic delivery of an antibody–photoabsorber conjugate, followed by a 24-h waiting period to ensure adequate localisation on the target cells. Subsequent exposure to near-infrared (NIR) light selectively damages the target cells. We aimed to improve the efficacy of PIT in vivo by evaluating the effects of the different routes of conjugate administration on treatment outcomes. Methods: Subcutaneous Lewis lung carcinoma tumours were established in mice, targeting cluster of differentiation (CD)44 with an anti-CD44 antibody conjugated to IRDye700DX (IR700). The conjugate was administered via the intravenous or intratumoural route followed by the assessment of antibody binding and therapeutic effects of PIT. Findings: Compared to intravenous administration, intratumoural delivery of the CD44-IR700 conjugate significantly increased the number of cells binding to the conjugate by >five-fold. This method, combined with NIR light irradiation, halved tumour growth when compared to intravenous delivery. Reducing the interval between intratumoural injection and NIR light exposure to 30 min did not diminish efficacy, thereby demonstrating the feasibility of a 1-h procedure. Interpretation: Intratumoural administration of the antibody–photoabsorber conjugate enhanced the efficacy of PIT in vivo. A simplified, 1-h procedure involving conjugate tumour injection followed by irradiation emerged as a potent cancer treatment strategy. Funding: This study was supported by theJapan Society for the Promotion of Science, theJapan Agency for Medical Research and Development,Japan Science and Technology Agency, and theOsaka Medical Research Foundation for Intractable Diseases.
Publisher	Elsevier B.V.
Source Title	eBioMedicine
Volume (Issue)	112
Page	105566
Date of Issued	2025-02-01
Language	English
Handle URL	https://hdl.handle.net/11094/100994
eISSN	23523964
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.ebiom.2025.105566
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCTERMS.bibliographicCitation	eBioMedicine. 2025, 112, p. 105566
DC.title	Enhancing the efficacy of near-infrared photoimmunotherapy through intratumoural delivery of CD44–targeting antibody–photoabsorber conjugates
DC.creator	Adachi, Yuichi
	Miyake, Kotaro
	Ohira, Kika
	Satoh, Shingo
	Masuhiro, Kentaro
	Edahiro, Ryuya
	Shirai, Yuya
	Naito, Maiko
	Naito, Yujiro
	Shiroyama, Takayuki
	Koyama, Shohei
	Hirata, Haruhiko
	Iwahori, Kota
	Nagatomo, Izumi
	Takeda, Yoshito
	Kumanogoh, Atsushi
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-02-01
DC.identifier	https://doi.org/10.1016/j.ebiom.2025.105566
DC.subject	Antibody–photoabsorber conjugate
	Intratumoural administration
	Lung cancer
	Photoimmunotherapy
DC.description	Adachi Y., Miyake K., Ohira K., et al. Enhancing the efficacy of near-infrared photoimmunotherapy through intratumoural delivery of CD44–targeting antibody–photoabsorber conjugates. eBioMedicine 112, 105566 (2025); https://doi.org/10.1016/j.ebiom.2025.105566.
DCTERMS.abstract	Background: Photoimmunotherapy (PIT) is a potent modality for cancer treatment. The conventional PIT regimen involves the systemic delivery of an antibody–photoabsorber conjugate, followed by a 24-h waiting period to ensure adequate localisation on the target cells. Subsequent exposure to near-infrared (NIR) light selectively damages the target cells. We aimed to improve the efficacy of PIT in vivo by evaluating the effects of the different routes of conjugate administration on treatment outcomes. Methods: Subcutaneous Lewis lung carcinoma tumours were established in mice, targeting cluster of differentiation (CD)44 with an anti-CD44 antibody conjugated to IRDye700DX (IR700). The conjugate was administered via the intravenous or intratumoural route followed by the assessment of antibody binding and therapeutic effects of PIT. Findings: Compared to intravenous administration, intratumoural delivery of the CD44-IR700 conjugate significantly increased the number of cells binding to the conjugate by >five-fold. This method, combined with NIR light irradiation, halved tumour growth when compared to intravenous delivery. Reducing the interval between intratumoural injection and NIR light exposure to 30 min did not diminish efficacy, thereby demonstrating the feasibility of a 1-h procedure. Interpretation: Intratumoural administration of the antibody–photoabsorber conjugate enhanced the efficacy of PIT in vivo. A simplified, 1-h procedure involving conjugate tumour injection followed by irradiation emerged as a potent cancer treatment strategy. Funding: This study was supported by theJapan Society for the Promotion of Science, theJapan Agency for Medical Research and Development,Japan Science and Technology Agency, and theOsaka Medical Research Foundation for Intractable Diseases.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	100994
citation_title	Enhancing the efficacy of near-infrared photoimmunotherapy through intratumoural delivery of CD44–targeting antibody–photoabsorber conjugates
citation_author	Adachi, Yuichi
	Miyake, Kotaro
	Ohira, Kika
	Satoh, Shingo
	Masuhiro, Kentaro
	Edahiro, Ryuya
	Shirai, Yuya
	Naito, Maiko
	Naito, Yujiro
	Shiroyama, Takayuki
	Koyama, Shohei
	Hirata, Haruhiko
	Iwahori, Kota
	Nagatomo, Izumi
	Takeda, Yoshito
	Kumanogoh, Atsushi
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2025-02-01
citation_journal_title	eBioMedicine
citation_volume	112
citation_firstpage	105566
citation_doi	https://doi.org/10.1016/j.ebiom.2025.105566
citation_public_url	https://hdl.handle.net/11094/100994

Enhancing the efficacy of near-infrared photoimmunotherapy through intratumoural delivery of CD44–targeting antibody–photoabsorber conjugates

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