Correlation between the metabolic profile of Nelumbo Seed, a component of Seishinrenshiin, and its inhibitory activity on bladder smooth muscle contraction

Harada, Kazuo; Fukuda, Yuki; Ohkubo, Takahiro et al.

Journal of Natural Medicines, 2025

Number of Access:290（2025-08-14 02:03 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/101008

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Title
Correlation between the metabolic profile of Nelumbo Seed, a component of Seishinrenshiin, and its inhibitory activity on bladder smooth muscle contraction
Creator
NRID
1000050397741 1000050397741
ORCID
0000-0002-9348-1629 0000-0002-9348-1629
researchmap permalink
read0204933 read0204933
Scopus Author ID
16549528100 16549528100
CreatorName
Harada, Kazuo
CreatorName
Fukuda, Yuki
CreatorName
Ohkubo, Takahiro
NRID
1000020179120 1000020179120
CreatorName
Sugaya, Kimio
CreatorName
Osaki, Yukihiko
Subject
Bladder smooth muscle
Metabolic profiling
Neferine
Nelumbo Seed
Seishinrenshiin
Description
The version of record of this article, first published in Journal of Natural Medicines, is available online at Publisher’s website: https://doi.org/10.1007/s11418-025-01889-4
Abstract
Seishinrenshiin (SRI), a Kampo formula, is often used for frequent urination. Nelumbo Seed, a crude-drug component of SRI was reported to inhibit bladder smooth muscle contraction using excised rat bladder tissues. However, the active ingredients of Nelumbo Seed have not yet been identified. In this study, we investigated the active ingredients of Nelumbo Seed that inhibit bladder smooth muscle contraction. We obtained liquid chromatography/mass spectrometry profiles of extracts prepared from five types of Nelumbo Seed materials from different production areas and evaluated their inhibitory effects on excised rat bladder smooth muscle contraction. Analysis of these data using orthogonal projections to latent structures revealed neferine (Nef) as the compound with the highest variable influence on projection. Among the Nelumbo Seed materials processed using different methods, the Nef content was the highest in ‘Sekirenshi’, when embryo and pericarp were not removed at all. We determined the effects of administering Nelumbo Seed extracts with different Nef content on frequent-urination model rats. The Nef content in rat plasma was the highest when the Sekirenshi extract was administered, but Nef was not detected when seed embryos were removed. In this model, administration of the Nelumbo Seed extract improved the maximum bladder contraction pressure and bladder contraction interval, albeit not significantly. In particular, Sekirenshi tended to reduce the maximum bladder contraction pressure compared to other Nelumbo Seed. Our results indicate that although Nef in Nelumbo Seed does not clearly improve frequent urination, it might contribute to the improvement of urination disorder.
Publisher
Springer
Source Title
Journal of Natural Medicines
Date of Issued
2025-03-19
Language
English
Handle URL
https://hdl.handle.net/11094/101008
PISSN
13403443
eISSN
18610293
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1007/s11418-025-01889-4
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
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Version of Record
Category
学術雑誌論文 Journal Article