Ex-vivo Imaging of Glial Energy Metabolism in the Neonatal Mouse Brain during Convulsive Seizures with Intranasal Radiotracer Administration

Hosoi, Rie; Tada, Kenya; Hayakawa, Takahiro et al.

Molecular Imaging and Biology, 2025

Number of Access:218（2025-11-05 06:31 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/101011

Link to primary information 

File Format Terms of use Size Views Date.Available Description information
MolImagingBiol pdf None 2.41 MB 22 2025.04.17  
Creative Commons : Attribution

Item Information

Output File Export EndNote Basic Export Mendeley

Title
Ex-vivo Imaging of Glial Energy Metabolism in the Neonatal Mouse Brain during Convulsive Seizures with Intranasal Radiotracer Administration
Creator
NRID
1000030291446 1000030291446
researchmap permalink
read0051667 read0051667
Scopus Author ID
6701916426 6701916426
CreatorName
Hosoi, Rie
CreatorName
Tada, Kenya
CreatorName
Hayakawa, Takahiro
CreatorName
Haga, Yuka
Subject
Glia
Intranasal administration
Neonatal mouse brain
Pentylenetetrazole
Seizure
Description
The version of record of this article, first published in Molecular Imaging and Biology, is available online at Publisher’s website: https://doi.org/10.1007/s11307-025-02000-9
Abstract
Purpose: In this study, we examined changes in glial energy metabolism in neonatal mouse brain images obtained under pathological conditions following intranasal administration of the radiotracer [2-14C]acetate. Procedures: [2-14C]acetate was administered via the mouse nasal cavity, after which autoradiograms of the brain of 7-day-old mice were obtained. Radio thin-layer chromatography was applied for metabolite analysis of brain radioactivity. We also compared brain uptake of [2-14C]acetate when administrated intranasally and intravenously in 3-week-old mice. To confirm selective uptake by glial cells, [2-14C]acetate was injected into the nasal cavity of mice injected with a glial toxin in the brain. Pentylenetetrazole (PTZ) was applied to induce seizures. Results: Intranasally administered [2-14C]acetate was rapidly incorporated into the brains of 7-day-old mice, reaching its highest uptake level 20 min after administration. After 20 min of intranasal [2-14C]acetate administration, glutamate and glutamine accounted for 32 ± 2.5% and 30 ± 3.4% of total brain radioactivity, respectively. There was no difference in the radioactivity distribution in the brain between intranasal and intravenous administration, except in the ventral olfactory bulb in 3-week-old mice. Microinjection of the glial-specific toxin fluorocitrate reduced the accumulation of radioactivity in the brain by 60% following intranasal administration in 3-week-old mice. The uptake of [2-14C]acetate in the brains of 7-day-old mice significantly decreased 30 min after systemic PTZ administration, suggesting a decrease in energy metabolism in glial cells during seizures. Conclusions: Quantitative images of biological functions in the neonatal mouse brain can be obtained by intranasal administration. This technique allowed the observation of a decrease in acetate uptake associated with convulsive seizures. The results of this study could be applied to the imaging of biological brain functions and research on neurological disorders using labeled probes in neonatal mice.
Publisher
Springer Science and Business Media Deutschland GmbH
Source Title
Molecular Imaging and Biology
Date of Issued
2025-03-25
Language
English
Handle URL
https://hdl.handle.net/11094/101011
PISSN
15361632
eISSN
18602002
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1007/s11307-025-02000-9
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article