Basic research for ultrasound-guided injection into skeletal muscle lesions in an experimental animal model

Fujimoto, K.; Kanamoto, Takashi; Otani, S.; Miyazaki, R.; Ebina, K.; Nakata, K.

doi:https://doi.org/10.1302/2046-3758.141.BJR-2024-0090.R1

Basic research for ultrasound-guided injection into skeletal muscle lesions in an experimental animal model

Fujimoto, K.; Kanamoto, Takashi; Otani, S. et al.

Bone and Joint Research, 2025, 14(1), 33-41

Number of Access:691（2025-08-14 12:00 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/101066

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Title	Basic research for ultrasound-guided injection into skeletal muscle lesions in an experimental animal model
Creator	CreatorName Fujimoto, K.
	NRID 1000020512049 1000020512049 researchmap permalink kanamoto kanamoto Scopus Author ID 7005012135 7005012135 CreatorName Kanamoto, Takashi
	CreatorName Otani, S.
	CreatorName Miyazaki, R.
	NRID 1000070612076 1000070612076 ORCID 0000-0002-2426-1024 0000-0002-2426-1024 researchmap permalink k-ebina k-ebina Scopus Author ID 8415453300 8415453300 CreatorName Ebina, K.
	NRID 1000000283747 1000000283747 researchmap permalink ken-nakata ken-nakata Scopus Author ID 7402151308 7402151308 CreatorName Nakata, K.
Description	Fujimoto K., Kanamoto T., Otani S., et al. Basic research for ultrasound-guided injection into skeletal muscle lesions in an experimental animal model. Bone and Joint Research 14, 33 (2025); https://doi.org/10.1302/2046-3758.141.BJR-2024-0090.R1.
Abstract	Aims Ultrasound-guided injection techniques are expected to enhance therapeutic efficacy for skeletal muscle injuries and disorders, but basic knowledge is lacking. The purpose of this study was to examine the diagnostic accuracy of ultrasound for abnormal skeletal muscle lesions, and to examine the distribution patterns of solution and cells injected into abnormal muscle lesions under ultrasound guidance. Methods A cardiotoxin (CTX)-induced muscle injury model was used. Briefly, CTX was injected into tibialis anterior muscle in rats under ultrasound observation. First, the diagnostic accuracy of abnormal muscle lesions on ultrasound was examined by comparing ultrasound findings and histology. Next, Fast Green solution and green fluorescent protein (GFP)-labelled cells were simultaneously injected into the abnormal muscle lesions under ultrasound guidance, and their distribution was evaluated. Results Evaluation of short-axis ultrasound images and cross-sectional histological staining showed a strong correlation (r = 0.927; p < 0.001) between the maximum muscle damage area in ultrasound and haematoxylin and eosin (H&E) staining evaluations. Histological analysis showed that ultrasound-guided injection could successfully deliver Fast Green solution around the myofibres at the site of injury. In contrast, the distribution of injected cells was very localized compared to the area stained with Fast Green. Conclusion This experimental animal study demonstrated the potential of ultrasound to quantitatively visualize abnormalities of skeletal muscle. It also showed that ultrasound-guided injections allowed for highly accurate distribution of solution and cells in abnormal muscle tissue, but the patterns of solution and cell distribution were markedly different. Although future studies using a more clinically relevant model are necessary, these results are important findings when considering biological therapies for skeletal muscle injuries and disorders.
Publisher	British Editorial Society of Bone and Joint Surgery
Source Title	Bone and Joint Research
Volume (Issue)	14(1)
Page	33-41
Date of Issued	2025-01-17
Language	English
Handle URL	https://hdl.handle.net/11094/101066
eISSN	20463758
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1302/2046-3758.141.BJR-2024-0090.R1
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Bone and Joint Research. 2025, 14(1), p. 33-41
DC.title	Basic research for ultrasound-guided injection into skeletal muscle lesions in an experimental animal model
DC.creator	Fujimoto, K.
	Kanamoto, Takashi
	Otani, S.
	Miyazaki, R.
	Ebina, K.
	Nakata, K.
DC.publisher	British Editorial Society of Bone and Joint Surgery
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-01-17
DC.identifier	https://doi.org/10.1302/2046-3758.141.BJR-2024-0090.R1
DC.description	Fujimoto K., Kanamoto T., Otani S., et al. Basic research for ultrasound-guided injection into skeletal muscle lesions in an experimental animal model. Bone and Joint Research 14, 33 (2025); https://doi.org/10.1302/2046-3758.141.BJR-2024-0090.R1.
DCTERMS.abstract	Aims Ultrasound-guided injection techniques are expected to enhance therapeutic efficacy for skeletal muscle injuries and disorders, but basic knowledge is lacking. The purpose of this study was to examine the diagnostic accuracy of ultrasound for abnormal skeletal muscle lesions, and to examine the distribution patterns of solution and cells injected into abnormal muscle lesions under ultrasound guidance. Methods A cardiotoxin (CTX)-induced muscle injury model was used. Briefly, CTX was injected into tibialis anterior muscle in rats under ultrasound observation. First, the diagnostic accuracy of abnormal muscle lesions on ultrasound was examined by comparing ultrasound findings and histology. Next, Fast Green solution and green fluorescent protein (GFP)-labelled cells were simultaneously injected into the abnormal muscle lesions under ultrasound guidance, and their distribution was evaluated. Results Evaluation of short-axis ultrasound images and cross-sectional histological staining showed a strong correlation (r = 0.927; p < 0.001) between the maximum muscle damage area in ultrasound and haematoxylin and eosin (H&E) staining evaluations. Histological analysis showed that ultrasound-guided injection could successfully deliver Fast Green solution around the myofibres at the site of injury. In contrast, the distribution of injected cells was very localized compared to the area stained with Fast Green. Conclusion This experimental animal study demonstrated the potential of ultrasound to quantitatively visualize abnormalities of skeletal muscle. It also showed that ultrasound-guided injections allowed for highly accurate distribution of solution and cells in abnormal muscle tissue, but the patterns of solution and cell distribution were markedly different. Although future studies using a more clinically relevant model are necessary, these results are important findings when considering biological therapies for skeletal muscle injuries and disorders.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	101066
citation_title	Basic research for ultrasound-guided injection into skeletal muscle lesions in an experimental animal model
citation_author	Fujimoto, K.
	Kanamoto, Takashi
	Otani, S.
	Miyazaki, R.
	Ebina, K.
	Nakata, K.
citation_publisher	British Editorial Society of Bone and Joint Surgery
citation_language	English
citation_date	2025-01-17
citation_journal_title	Bone and Joint Research
citation_volume	14
citation_issue	1
citation_firstpage	33
citation_lastpage	41
citation_doi	https://doi.org/10.1302/2046-3758.141.BJR-2024-0090.R1
citation_public_url	https://hdl.handle.net/11094/101066

Basic research for ultrasound-guided injection into skeletal muscle lesions in an experimental animal model

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