Ethiodized Oil Emulsion for Sustained Release of Anti–PD-L1 Antibodies

Satomura, Hiroki; Kimura, Yasushi; Kennoki, Norifumi; Tomotake, Kosuke; Yano, Hiroki; Koretsune, Yuji; Katayama, Daisuke; Tanaka, Kaishu; Ono, Yusuke; Higashihara, Hiroki; Tomiyama, Noriyuki

doi:https://doi.org/10.1016/j.jvir.2025.01.040

Ethiodized Oil Emulsion for Sustained Release of Anti–PD-L1 Antibodies

Satomura, Hiroki; Kimura, Yasushi; Kennoki, Norifumi et al.

Journal of Vascular and Interventional Radiology, 2025, 36(5), 861-868.e2

Number of Access:927（2025-07-27 09:10 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/101080

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Title	Ethiodized Oil Emulsion for Sustained Release of Anti–PD-L1 Antibodies
Creator	CreatorName Satomura, Hiroki
	NRID 1000080774223 1000080774223 researchmap permalink kimurayasushi kimurayasushi CreatorName Kimura, Yasushi
	researchmap permalink HepaSphere HepaSphere CreatorName Kennoki, Norifumi
	CreatorName Tomotake, Kosuke
	CreatorName Yano, Hiroki
	CreatorName Koretsune, Yuji
	researchmap permalink daisuke_katayama daisuke_katayama CreatorName Katayama, Daisuke
	NRID 1000050839701 1000050839701 researchmap permalink ushuika- ushuika- Scopus Author ID 55311437600 55311437600 CreatorName Tanaka, Kaishu
	NRID 1000090816901 1000090816901 researchmap permalink y-ono y-ono Scopus Author ID 56406831000 56406831000 CreatorName Ono, Yusuke
	NRID 1000090423186 1000090423186 researchmap permalink hiroki-portal hiroki-portal Scopus Author ID 13605077500 13605077500 CreatorName Higashihara, Hiroki
	NRID 1000050294070 1000050294070 researchmap permalink 3430 3430 Scopus Author ID 7006024942 7006024942 CreatorName Tomiyama, Noriyuki
Description	Satomura H., Kimura Y., Kennoki N., et al. Ethiodized Oil Emulsion for Sustained Release of Anti–PD-L1 Antibodies. Journal of Vascular and Interventional Radiology , (2025); https://doi.org/10.1016/j.jvir.2025.01.040.
Abstract	Purpose: To develop and assess an ethiodized oil (Lipiodol) Pickering emulsion containing anti–programmed cell death ligand 1 (PD-L1) antibodies through in vitro experiments. Materials and Methods: The emulsion was created by combining ethiodized oil with poly (lactic-co-glycolic acid) (PLGA) nanoparticles and anti–PD-L1 antibodies. Confocal laser microscopy was used to evaluate the encapsulation of the antibodies within the Pickering emulsion. To assess the stability, the emulsion was visually examined, and droplet sizes were measured under a light microscope. For the sustained release evaluation, the emulsion was introduced into saline and incubated in a shaking bath, after which the supernatant was collected over time. The concentration of anti–PD-L1 antibodies in the supernatant was determined using a bicinchoninic acid assay. Western blotting and flow cytometry were employed to confirm the functionality of the released antibodies. A conventional ethiodized oil emulsion was used as a control for comparison. Results: The anti–PD-L1 antibodies were encapsulated within the layer of PLGA nanoparticles, positioned at the interface between the water and oil phases, as confirmed by confocal laser microscopy. The ethiodized oil Pickering emulsion demonstrated long-term stability with significantly smaller droplet sizes (P < .001). Moreover, the emulsion facilitated a gradual and sustained release of the anti–PD-L1 antibodies over an 8-week period (P < .001). The antibodies released from the emulsion specifically targeted PD-L1. Conclusions: This study demonstrated that ethiodized oil Pickering emulsions effectively encapsulate anti–PD-L1 antibodies and enable their sustained release, highlighting their potential as a therapeutic agent for primary and secondary liver cancers.
Publisher	Elsevier Inc.
Source Title	Journal of Vascular and Interventional Radiology
Volume (Issue)	36(5)
Page	861-868.e2
Date of Issued	2025-05
Language	English
Handle URL	https://hdl.handle.net/11094/101080
PISSN	10510443
eISSN	15357732
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.jvir.2025.01.040
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Journal of Vascular and Interventional Radiology. 2025, 36(5), p. 861-868.e2
DC.title	Ethiodized Oil Emulsion for Sustained Release of Anti–PD-L1 Antibodies
DC.creator	Satomura, Hiroki
	Kimura, Yasushi
	Kennoki, Norifumi
	Tomotake, Kosuke
	Yano, Hiroki
	Koretsune, Yuji
	Katayama, Daisuke
	Tanaka, Kaishu
	Ono, Yusuke
	Higashihara, Hiroki
	Tomiyama, Noriyuki
DC.publisher	Elsevier Inc.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-05
DC.identifier	https://doi.org/10.1016/j.jvir.2025.01.040
DC.description	Satomura H., Kimura Y., Kennoki N., et al. Ethiodized Oil Emulsion for Sustained Release of Anti–PD-L1 Antibodies. Journal of Vascular and Interventional Radiology , (2025); https://doi.org/10.1016/j.jvir.2025.01.040.
DCTERMS.abstract	Purpose: To develop and assess an ethiodized oil (Lipiodol) Pickering emulsion containing anti–programmed cell death ligand 1 (PD-L1) antibodies through in vitro experiments. Materials and Methods: The emulsion was created by combining ethiodized oil with poly (lactic-co-glycolic acid) (PLGA) nanoparticles and anti–PD-L1 antibodies. Confocal laser microscopy was used to evaluate the encapsulation of the antibodies within the Pickering emulsion. To assess the stability, the emulsion was visually examined, and droplet sizes were measured under a light microscope. For the sustained release evaluation, the emulsion was introduced into saline and incubated in a shaking bath, after which the supernatant was collected over time. The concentration of anti–PD-L1 antibodies in the supernatant was determined using a bicinchoninic acid assay. Western blotting and flow cytometry were employed to confirm the functionality of the released antibodies. A conventional ethiodized oil emulsion was used as a control for comparison. Results: The anti–PD-L1 antibodies were encapsulated within the layer of PLGA nanoparticles, positioned at the interface between the water and oil phases, as confirmed by confocal laser microscopy. The ethiodized oil Pickering emulsion demonstrated long-term stability with significantly smaller droplet sizes (P < .001). Moreover, the emulsion facilitated a gradual and sustained release of the anti–PD-L1 antibodies over an 8-week period (P < .001). The antibodies released from the emulsion specifically targeted PD-L1. Conclusions: This study demonstrated that ethiodized oil Pickering emulsions effectively encapsulate anti–PD-L1 antibodies and enable their sustained release, highlighting their potential as a therapeutic agent for primary and secondary liver cancers.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	101080
citation_title	Ethiodized Oil Emulsion for Sustained Release of Anti–PD-L1 Antibodies
citation_author	Satomura, Hiroki
	Kimura, Yasushi
	Kennoki, Norifumi
	Tomotake, Kosuke
	Yano, Hiroki
	Koretsune, Yuji
	Katayama, Daisuke
	Tanaka, Kaishu
	Ono, Yusuke
	Higashihara, Hiroki
	Tomiyama, Noriyuki
citation_publisher	Elsevier Inc.
citation_language	English
citation_date	2025-05
citation_journal_title	Journal of Vascular and Interventional Radiology
citation_volume	36
citation_issue	5
citation_firstpage	861
citation_lastpage	868.e2
citation_doi	https://doi.org/10.1016/j.jvir.2025.01.040
citation_public_url	https://hdl.handle.net/11094/101080

Ethiodized Oil Emulsion for Sustained Release of Anti–PD-L1 Antibodies

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