Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis

Kidawara, Sota; Kimura, Shizuki; Takano, Haruka; Inoue, Wakana; Iinuma, Kota; Takahama, Michihiro; Takemura, Naoki; Saitoh, Tatsuya

doi:https://doi.org/10.1248/bpb.b24-00594

Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis

Kidawara, Sota; Kimura, Shizuki; Takano, Haruka et al.

Biological and Pharmaceutical Bulletin, 2024, 47(11), 1920-1926

Number of Access:361（2025-08-16 00:38 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/101357

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Title	Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis
Creator	CreatorName Kidawara, Sota
	CreatorName Kimura, Shizuki
	CreatorName Takano, Haruka
	CreatorName Inoue, Wakana
	CreatorName Iinuma, Kota
	NRID 1000010774755 1000010774755 researchmap permalink mtakahama mtakahama CreatorName Takahama, Michihiro
	NRID 1000050648699 1000050648699 researchmap permalink ntakemura ntakemura Scopus Author ID 57190744932 57190744932 CreatorName Takemura, Naoki
	NRID 1000060456936 1000060456936 researchmap permalink saitohtatsuya saitohtatsuya Scopus Author ID 7401688725 7401688725 CreatorName Saitoh, Tatsuya
Subject	allergic rhinitis
	degranulation
	mast cell
	oridonin
Description	Kidawara S., Kimura S., Takano H., et al. Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis. Biological and Pharmaceutical Bulletin 47, 1920 (2024); https://doi.org/10.1248/bpb.b24-00594.
Abstract	Incidence of type I allergies, such as hay fever, is continuously increasing in developed countries, including Japan. Type I allergies are triggered by chemical mediators, such as histamine, which are released via immunoglobulin E (IgE)-mediated mast cell degranulation. Therefore, medications inhibiting the synthesis, release, and receptor binding of these mediators are commonly used to manage type I allergy symptoms. As self-care disease prevention practices are gaining attention worldwide, regular consumption of food and supplements containing safe components inhibiting mast cell degranulation is a potential strategy to prevent allergic attacks. Here, we aimed to assess the ability of phytochemicals derived from edible plants to inhibit mast cell degranulation using the β-hexosaminidase release assay and investigate their cytotoxicity and efficiency in alleviating allergic symptoms. We found that oridonin, a diterpenoid isolated from Isodon japonicus Hara, strongly inhibited β-hexosaminidase release from both the RBL-2H3 rat cell line and mouse bone marrow-derived mast cells stimulated with dinitrophenyl (DNP)-conjugated human serum albumin after sensitization with DNP-IgE. Oridonin also inhibited β-hexosaminidase release induced by the calcium ionophore, A23187, in both cell types. Notably, oridonin did not adversely affect cell survival at concentrations necessary to inhibit β-hexosaminidase release. In a mouse model of ovalbumin (OVA)-induced allergic rhinitis, intraperitoneal administration of oridonin significantly reduced the nasal rubbing caused by intranasal OVA administration without affecting the serum levels of OVA-specific IgE. Therefore, oridonin could be an effective daily intake component to alleviate allergic diseases by inhibiting mast cell degranulation.
Publisher	Pharmaceutical Society of Japan
Source Title	Biological and Pharmaceutical Bulletin
Volume (Issue)	47(11)
Page	1920-1926
Date of Issued	2024-11-01
Language	English
Handle URL	https://hdl.handle.net/11094/101357
PISSN	09186158
eISSN	13475215
Relation.isSupplementedBy	DOI (Publisher Version) https://doi.org/10.60574/101358
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1248/bpb.b24-00594
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Biological and Pharmaceutical Bulletin. 2024, 47(11), p. 1920-1926
DC.title	Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis
DC.creator	Kidawara, Sota
	Kimura, Shizuki
	Takano, Haruka
	Inoue, Wakana
	Iinuma, Kota
	Takahama, Michihiro
	Takemura, Naoki
	Saitoh, Tatsuya
DC.publisher	Pharmaceutical Society of Japan
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-11-01
DC.identifier	https://doi.org/10.1248/bpb.b24-00594
DC.identifier	https://doi.org/10.60574/101358
DC.subject	allergic rhinitis
	degranulation
	mast cell
	oridonin
DC.description	Kidawara S., Kimura S., Takano H., et al. Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis. Biological and Pharmaceutical Bulletin 47, 1920 (2024); https://doi.org/10.1248/bpb.b24-00594.
DCTERMS.abstract	Incidence of type I allergies, such as hay fever, is continuously increasing in developed countries, including Japan. Type I allergies are triggered by chemical mediators, such as histamine, which are released via immunoglobulin E (IgE)-mediated mast cell degranulation. Therefore, medications inhibiting the synthesis, release, and receptor binding of these mediators are commonly used to manage type I allergy symptoms. As self-care disease prevention practices are gaining attention worldwide, regular consumption of food and supplements containing safe components inhibiting mast cell degranulation is a potential strategy to prevent allergic attacks. Here, we aimed to assess the ability of phytochemicals derived from edible plants to inhibit mast cell degranulation using the β-hexosaminidase release assay and investigate their cytotoxicity and efficiency in alleviating allergic symptoms. We found that oridonin, a diterpenoid isolated from Isodon japonicus Hara, strongly inhibited β-hexosaminidase release from both the RBL-2H3 rat cell line and mouse bone marrow-derived mast cells stimulated with dinitrophenyl (DNP)-conjugated human serum albumin after sensitization with DNP-IgE. Oridonin also inhibited β-hexosaminidase release induced by the calcium ionophore, A23187, in both cell types. Notably, oridonin did not adversely affect cell survival at concentrations necessary to inhibit β-hexosaminidase release. In a mouse model of ovalbumin (OVA)-induced allergic rhinitis, intraperitoneal administration of oridonin significantly reduced the nasal rubbing caused by intranasal OVA administration without affecting the serum levels of OVA-specific IgE. Therefore, oridonin could be an effective daily intake component to alleviate allergic diseases by inhibiting mast cell degranulation.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	101357
citation_title	Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis
citation_author	Kidawara, Sota
	Kimura, Shizuki
	Takano, Haruka
	Inoue, Wakana
	Iinuma, Kota
	Takahama, Michihiro
	Takemura, Naoki
	Saitoh, Tatsuya
citation_publisher	Pharmaceutical Society of Japan
citation_language	English
citation_date	2024-11-01
citation_journal_title	Biological and Pharmaceutical Bulletin
citation_volume	47
citation_issue	11
citation_firstpage	1920
citation_lastpage	1926
citation_doi	https://doi.org/10.1248/bpb.b24-00594
citation_doi	https://doi.org/10.60574/101358
citation_public_url	https://hdl.handle.net/11094/101357

Oridonin Suppresses Mast Cell Degranulation and Alleviates Ovalbumin-Induced Allergic Rhinitis

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