Comparative prospective study on the clinical utility of G-banding and next-generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan

Honda, Hidemine; Takiuchi, Tsuyoshi; Handa, Mika; Wakui, Nao; Tsuji, Saori; Goto, Takeshi; Suzuki, Shota; Saji, Fumie; Miyake, Tatsuya; Goto, Sakae; Okamura, Satomi; Yamada, Tomomi; Kodama, Michiko; Kimura, Tadashi

doi:https://doi.org/10.1002/rmb2.12655

Comparative prospective study on the clinical utility of G-banding and next-generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan

Honda, Hidemine; Takiuchi, Tsuyoshi; Handa, Mika et al.

Reproductive Medicine and Biology, 2025, 24(1), e12655

Number of Access:563（2025-07-18 17:49 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/101404

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Title	Comparative prospective study on the clinical utility of G-banding and next-generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan
Creator	CreatorName Honda, Hidemine
	NRID 1000040733358 1000040733358 researchmap permalink Takiuchi-gyne Takiuchi-gyne Scopus Author ID 51764431400 51764431400 CreatorName Takiuchi, Tsuyoshi
	researchmap permalink mikahanda mikahanda CreatorName Handa, Mika
	CreatorName Wakui, Nao
	CreatorName Tsuji, Saori
	CreatorName Goto, Takeshi
	CreatorName Suzuki, Shota
	CreatorName Saji, Fumie
	NRID 1000000814766 1000000814766 researchmap permalink a-1 a-1 Scopus Author ID 57188931396 57188931396 CreatorName Miyake, Tatsuya
	NRID 1000070293826 1000070293826 CreatorName Goto, Sakae
	CreatorName Okamura, Satomi
	NRID 1000060363371 1000060363371 researchmap permalink ymd ymd Scopus Author ID 35254679600 35254679600 CreatorName Yamada, Tomomi
	NRID 1000070791391 1000070791391 ORCID 0000-0003-4046-7572 0000-0003-4046-7572 researchmap permalink mkyh mkyh Scopus Author ID 35422516200 35422516200 CreatorName Kodama, Michiko
	NRID 1000090240845 1000090240845 CreatorName Kimura, Tadashi
Subject	fetal chromosomal abnormalities
	G-banding
	miscarriage
	next-generation sequencing
	products of conception
Description	Honda H., Takiuchi T., Handa M., et al. Comparative prospective study on the clinical utility of G-banding and next-generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan. Reproductive Medicine and Biology 24, e12655 (2025); https://doi.org/10.1002/rmb2.12655.
Abstract	Purpose: To compare the clinical utility of G-banding and next-generation sequencing (NGS) for chromosomal analysis of products of conception (POC), a crucial tool for detecting fetal chromosomal abnormalities which are major causes of miscarriage and stillbirth. Methods: We evaluated the clinical utility of both techniques in a prospective analysis of 40 patients who experienced miscarriages or stillbirths between 6 and 36 weeks of gestation under Advanced Medical Care A in Japan. Both methods were applied to the same POC samples. The primary outcome was the proportion of patients with a presumed cause of miscarriage or stillbirth among all submitted samples. Results: NGS presumed the cause in 75.0% (30/40) of cases, significantly outperforming G-banding's 42.5% (17/40) (p < 0.01). G-banding could analyze 67.5% (27/40) of the samples owing to culture failure, whereas NGS successfully analyzed all samples (100%, 40/40) (p < 0.01). Among the successfully analyzed samples, NGS presumed the cause in 70.3% (19/27) of cases, compared with 62.9% (17/27) for G-banding (p = 0.31). For miscarriages before 12 weeks, NGS presumed the cause in 73.5% (25/34) of cases, significantly higher than the 44.1% (15/34) (p < 0.01) presumed using G-banding. Conclusions: These results highlight the superior efficacy of NGS over G-banding for presuming causes of miscarriage or stillbirth.
Publisher	John Wiley and Sons Ltd
Source Title	Reproductive Medicine and Biology
Volume (Issue)	24(1)
Page	e12655
Date of Issued	2025-04-30
Language	English
Handle URL	https://hdl.handle.net/11094/101404
PISSN	14455781
eISSN	14470578
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1002/rmb2.12655
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Reproductive Medicine and Biology. 2025, 24(1), p. e12655
DC.title	Comparative prospective study on the clinical utility of G-banding and next-generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan
DC.creator	Honda, Hidemine
	Takiuchi, Tsuyoshi
	Handa, Mika
	Wakui, Nao
	Tsuji, Saori
	Goto, Takeshi
	Suzuki, Shota
	Saji, Fumie
	Miyake, Tatsuya
	Goto, Sakae
	Okamura, Satomi
	Yamada, Tomomi
	Kodama, Michiko
	Kimura, Tadashi
DC.publisher	John Wiley and Sons Ltd
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-04-30
DC.identifier	https://doi.org/10.1002/rmb2.12655
DC.subject	fetal chromosomal abnormalities
	G-banding
	miscarriage
	next-generation sequencing
	products of conception
DC.description	Honda H., Takiuchi T., Handa M., et al. Comparative prospective study on the clinical utility of G-banding and next-generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan. Reproductive Medicine and Biology 24, e12655 (2025); https://doi.org/10.1002/rmb2.12655.
DCTERMS.abstract	Purpose: To compare the clinical utility of G-banding and next-generation sequencing (NGS) for chromosomal analysis of products of conception (POC), a crucial tool for detecting fetal chromosomal abnormalities which are major causes of miscarriage and stillbirth. Methods: We evaluated the clinical utility of both techniques in a prospective analysis of 40 patients who experienced miscarriages or stillbirths between 6 and 36 weeks of gestation under Advanced Medical Care A in Japan. Both methods were applied to the same POC samples. The primary outcome was the proportion of patients with a presumed cause of miscarriage or stillbirth among all submitted samples. Results: NGS presumed the cause in 75.0% (30/40) of cases, significantly outperforming G-banding's 42.5% (17/40) (p < 0.01). G-banding could analyze 67.5% (27/40) of the samples owing to culture failure, whereas NGS successfully analyzed all samples (100%, 40/40) (p < 0.01). Among the successfully analyzed samples, NGS presumed the cause in 70.3% (19/27) of cases, compared with 62.9% (17/27) for G-banding (p = 0.31). For miscarriages before 12 weeks, NGS presumed the cause in 73.5% (25/34) of cases, significantly higher than the 44.1% (15/34) (p < 0.01) presumed using G-banding. Conclusions: These results highlight the superior efficacy of NGS over G-banding for presuming causes of miscarriage or stillbirth.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	101404
citation_title	Comparative prospective study on the clinical utility of G-banding and next-generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan
citation_author	Honda, Hidemine
	Takiuchi, Tsuyoshi
	Handa, Mika
	Wakui, Nao
	Tsuji, Saori
	Goto, Takeshi
	Suzuki, Shota
	Saji, Fumie
	Miyake, Tatsuya
	Goto, Sakae
	Okamura, Satomi
	Yamada, Tomomi
	Kodama, Michiko
	Kimura, Tadashi
citation_publisher	John Wiley and Sons Ltd
citation_language	English
citation_date	2025-04-30
citation_journal_title	Reproductive Medicine and Biology
citation_volume	24
citation_issue	1
citation_firstpage	e12655
citation_doi	https://doi.org/10.1002/rmb2.12655
citation_public_url	https://hdl.handle.net/11094/101404

Comparative prospective study on the clinical utility of G-banding and next-generation sequencing for chromosomal analysis of products of conception under Advanced Medical Care A in Japan

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