Regulation of Kinetic Properties of GluR2 AMPA Receptor Channels by Alternative Splicing

Koike, Maki; Tsukada, Shota; Tsuzuki, Keisuke et al.

Journal of Neuroscience, 2000, 20(6), 2166-2174

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Identifier to cite or link to this item: https://hdl.handle.net/11094/101969

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Title
Regulation of Kinetic Properties of GluR2 AMPA Receptor Channels by Alternative Splicing
Creator
NRID
1000060396702 1000060396702
ORCID
0000-0003-2679-5975 0000-0003-2679-5975
researchmap permalink
mktani mktani
CreatorName
Koike, Maki
CreatorName
Tsukada, Shota
CreatorName
Tsuzuki, Keisuke
NRID
1000030012397 1000030012397
CreatorName
Kijima, Hiromasa
CreatorName
Ozawa, Seiji
Subject
AMPA receptor channel
GluR2
Flip/flop splice variants
Q/R site
Desensitization
Deactivation
Description
Koike M., Tsukada S., Tsuzuki K., et al. Regulation of kinetic properties of GluR2 AMPA receptor channels by alternative splicing. Journal of Neuroscience 20, 2166 (2000); https://doi.org/10.1523/JNEUROSCI.20-06-02166.2000.
Abstract
The four subunits of the AMPA-type glutamate receptor (GluR1-GluR4 or GluR-A-GluR-D) exist in two distinct forms, flip and flop, generated by alternative splicing of a 115 bp region. The GluR2 subunit plays a key role in determining the functional properties of the AMPA receptor channel. In this study, we examined the differences in kinetic properties between the flip and flop splice variants of the GluR2 subunit expressed in Xenopus oocytes using fast agonist application techniques. Glutamate was applied to outside-out patches from oocytes with piezo-driven double-barreled application pipettes. Because homomeric receptor channels composed of the edited form of GluR2 (GluR2R) produce no appreciable current responses, we expressed the unedited form of GluR2 (GluR2Q) in oocytes, which produced large current responses sufficient for analysis of the kinetic properties. The time constant for desensitization during application of 1 mM glutamate was 5.89 ± 0.17 msec (n = 50) in flip and 1.18 ± 0.05 msec (n = 37) in flop. The deactivation time constant was 0.62 ± 0.06 msec (n = 10) in flip and 0.54 ± 0.05 msec (n = 10) in flop. The steady-state nondesensitizing current was 6.8 ± 0.4% (n = 53) of the peak current in flip, whereas it was almost negligible in flop, being only 1.1 ± 0.1% (n = 36). The slower desensitization kinetics and larger steady-state current responses in the flip variant were also observed in heteromeric receptors assembled from GluR2Q/GluR2R. Thus, desensitization occurred much more prominently in the flop variant in the recombinant GluR2 receptor channels.
Publisher
Society for Neuroscience
Source Title
Journal of Neuroscience
Volume (Issue)
20(6)
Page
2166-2174
Date of Issued
2000-03-15
Language
English
Handle URL
https://hdl.handle.net/11094/101969
PISSN
02706474
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1523/JNEUROSCI.20-06-02166.2000
Access Rights
open access
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article