Versatile confocal microscopy for imaging objects oscillating at frequencies exceeding the frame rate

Adachi, Kanta; Mukai, Seita; Higami, Kenichi; Saito, Takumi; Nakamura, Nobutomo

doi:https://doi.org/10.1063/5.0264700

Versatile confocal microscopy for imaging objects oscillating at frequencies exceeding the frame rate

Adachi, Kanta; Mukai, Seita; Higami, Kenichi et al.

Applied Physics Letters, 2025, 126(26), 263704

Number of Access:55（2025-08-15 06:46 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/102074

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Title	Versatile confocal microscopy for imaging objects oscillating at frequencies exceeding the frame rate
Creator	NRID 1000050823879 1000050823879 ORCID 0000-0002-5321-3777 0000-0002-5321-3777 researchmap permalink kantaadachi kantaadachi Scopus Author ID 57146267500 57146267500 CreatorName Adachi, Kanta
	CreatorName Mukai, Seita
	CreatorName Higami, Kenichi
	NRID 1000060967397 1000060967397 ORCID 0000-0001-6433-6302 0000-0001-6433-6302 researchmap permalink takumi_saito_zx takumi_saito_zx Scopus Author ID 57218441161 57218441161 CreatorName Saito, Takumi
	NRID 1000050452404 1000050452404 researchmap permalink nnakamura nnakamura Scopus Author ID 7403356186 7403356186 CreatorName Nakamura, Nobutomo
Description	K. Adachi et al., Appl. Phys. Lett. 126, 263704 (2025) https://doi.org/10.1063/5.0264700
Abstract	Confocal microscopy typically has a low frame rate but allows high-resolution imaging deep within objects. We developed a versatile method to capture confocal images of periodically moving objects at frequencies exceeding the frame rate. This method requires no additional equipment or compromise in imaging capabilities and is compatible with all confocal laser scanning microscopes. We have demonstrated its use to visualize colloidal particle motion during elastic wave propagation at 1600 Hz—two orders of magnitude faster than standard confocal microscopy. Particle oscillation amplitude and phase exhibited attenuation and delay with propagation distance, allowing the determination of the elastic wave's amplitude attenuation and phase velocity. The single-particle analysis further revealed that the direction of elastic wave propagation depended on the orientation of the colloidal grains.
Publisher	AIP Publishing
Source Title	Applied Physics Letters
Volume (Issue)	126(26)
Page	263704
Date of Issued	2025-07-01
Language	English
Handle URL	https://hdl.handle.net/11094/102074
PISSN	00036951
eISSN	10773118
Relation.isVersionOf	DOI (Publisher Version) https://doi.org/10.1063/5.0264700
Access Rights	open access
CopyRight	This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in (citation of published article) and may be found at (URL/link for published article abstract).
oaire:version	Accepted Manuscript
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Applied Physics Letters. 2025, 126(26), p. 263704
DC.title	Versatile confocal microscopy for imaging objects oscillating at frequencies exceeding the frame rate
DC.creator	Adachi, Kanta
	Mukai, Seita
	Higami, Kenichi
	Saito, Takumi
	Nakamura, Nobutomo
DC.publisher	AIP Publishing
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-07-01
DC.identifier	https://doi.org/10.1063/5.0264700
DC.description	K. Adachi et al., Appl. Phys. Lett. 126, 263704 (2025) https://doi.org/10.1063/5.0264700
DCTERMS.abstract	Confocal microscopy typically has a low frame rate but allows high-resolution imaging deep within objects. We developed a versatile method to capture confocal images of periodically moving objects at frequencies exceeding the frame rate. This method requires no additional equipment or compromise in imaging capabilities and is compatible with all confocal laser scanning microscopes. We have demonstrated its use to visualize colloidal particle motion during elastic wave propagation at 1600 Hz—two orders of magnitude faster than standard confocal microscopy. Particle oscillation amplitude and phase exhibited attenuation and delay with propagation distance, allowing the determination of the elastic wave's amplitude attenuation and phase velocity. The single-particle analysis further revealed that the direction of elastic wave propagation depended on the orientation of the colloidal grains.
DC.rights	This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in (citation of published article) and may be found at (URL/link for published article abstract).
DC.format	102074
citation_title	Versatile confocal microscopy for imaging objects oscillating at frequencies exceeding the frame rate
citation_author	Adachi, Kanta
	Mukai, Seita
	Higami, Kenichi
	Saito, Takumi
	Nakamura, Nobutomo
citation_publisher	AIP Publishing
citation_language	English
citation_date	2025-07-01
citation_journal_title	Applied Physics Letters
citation_volume	126
citation_issue	26
citation_firstpage	263704
citation_doi	https://doi.org/10.1063/5.0264700
citation_public_url	https://hdl.handle.net/11094/102074

Versatile confocal microscopy for imaging objects oscillating at frequencies exceeding the frame rate

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