Factories without walls: The molecular architecture and functions of non-membrane organelles in small RNA-guided genome protection

Kawaguchi, Shinichi; Isshiki, Wakana; Kai, Toshie

Biochimica et Biophysica Acta - General Subjects, 2025, 1869(7), 130811

Number of Access:41（2025-09-05 10:57 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/102197

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Title
Factories without walls: The molecular architecture and functions of non-membrane organelles in small RNA-guided genome protection
Creator
NRID
1000040321749 1000040321749
researchmap permalink
1234567890abc 1234567890abc
Scopus Author ID
36762092600 36762092600
CreatorName
Kawaguchi, Shinichi
CreatorName
Isshiki, Wakana
NRID
1000040579786 1000040579786
ORCID
0000-0001-8675-8469 0000-0001-8675-8469
researchmap permalink
toshie_kai toshie_kai
Scopus Author ID
7102037941 7102037941
CreatorName
Kai, Toshie
Subject
Non-membrane structure
Yb body
Nuage
piRNA
LLPS
Protein-protein interaction
AlphaFold3
Description
Kawaguchi S., Isshiki W., Kai T.. Factories without walls: The molecular architecture and functions of non-membrane organelles in small RNA-guided genome protection. Biochimica et Biophysica Acta - General Subjects 1869, 130811 (2025); https://doi.org/10.1016/j.bbagen.2025.130811.
Abstract
Non-membrane organelles, Yb body and nuage, play an essential role in piRNA-guided genome defense in Drosophila gonad by mediating piRNA biogenesis and transposon silencing. Yb body, found in somatic follicle cells, is responsible for primary piRNA processing, while nuage, located in germline cells, facilitates the ping-pong cycle to amplify the piRNAs corresponding to both sense and antisense strands of the expressed transposons. These organelles are assembled by liquid-liquid phase separation (LLPS) and protein-protein interactions, integrating RNA helicases (Vasa, Armitage), Tudor domain-containing proteins (Krimper, Tejas, Qin/Kumo), and proteins containing both domains (Yb, SoYb, Spn-E). Within these condensates, we summarize the protein-protein interactions experimentally validated and predicted by AlphaFold3, providing new structural insights into the non-membrane organelle assembly. This review highlights how the dynamic organization of Yb body and nuage enables efficient RNA processing, ensuring transposon suppression and genome stability.
Publisher
Elsevier B.V.
Source Title
Biochimica et Biophysica Acta - General Subjects
Volume (Issue)
1869(7)
Page
130811
Date of Issued
2025-05-03
Language
English
Handle URL
https://hdl.handle.net/11094/102197
PISSN
03044165
eISSN
18728006
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1016/j.bbagen.2025.130811
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article