Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging

Wee, Wen Ann; Andini, Tatum Melati; Kumagai, Tomotaka; Chinnathambi, Shanmugavel; Pandian, Ganesh N.; Millius, Arthur; Sugiyama, Hiroshi; Standley, Daron M.; Park, Soyoung

doi:https://doi.org/10.1016/j.aca.2025.344130

Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging

Wee, Wen Ann; Andini, Tatum Melati; Kumagai, Tomotaka et al.

Analytica Chimica Acta, 2025, 1359, 344130

Number of Access:14（2025-08-20 06:44 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/102199

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
AnalChimActa_1359	pdf	None	6.84 MB	28	2025.07.10

Creative Commons : Attribution - NonCommercial

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging
Creator	CreatorName Wee, Wen Ann
	CreatorName Andini, Tatum Melati
	CreatorName Kumagai, Tomotaka
	NRID 1000060922341 1000060922341 CreatorName Chinnathambi, Shanmugavel
	CreatorName Pandian, Ganesh N.
	NRID 1000080624858 1000080624858 CreatorName Millius, Arthur
	CreatorName Sugiyama, Hiroshi
	NRID 1000000448028 1000000448028 researchmap permalink 7000011861 7000011861 CreatorName Standley, Daron M.
	NRID 1000010628556 1000010628556 researchmap permalink 7000009174 7000009174 CreatorName Park, Soyoung
Subject	Fluorescent probe
	LD probe
	Lipid droplets
	Push-pull dye
	Solvatochromism
Description	Wee W.A., Andini T.M., Kumagai T., et al. Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging. Analytica Chimica Acta 1359, 344130 (2025); https://doi.org/10.1016/j.aca.2025.344130.
Abstract	Lipid droplets (LDs) are ubiquitous cellular organelles with a neutral lipid core containing triacylglycerols and cholesteryl esters surrounded by phospholipids. Recent findings indicate that LDs are intricately linked to diseases, such as cancer and neurological disorders, in addition to their roles in cellular senescence and immune responses. Herein, we describe a simple yet robust push-pull molecule, N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline (NiTA), as a versatile LD fluorescent probe. NiTA showed an absorption spectrum with a substantial bathochromic shift and a fluorescence spectrum with excellent solvatochromism. Leveraging the remarkable photophysical features of NiTA, we stained LDs in major immune cells, including T and B cells, and macrophages, and monitored the changes in LDs under oxidative and starvation conditions. Furthermore, we demonstrated the applicability of NiTA for visualizing the organization of medaka fish (Oryzias latipes) embryos during development. We expect the small yet powerful NiTA to be utilized in various applications, including fluorescence mapping to observe LD numbers, morphology, and polarity changes in animals and cells.
Publisher	Elsevier B.V.
Source Title	Analytica Chimica Acta
Volume (Issue)	1359
Page	344130
Date of Issued	2025-05-03
Language	English
Handle URL	https://hdl.handle.net/11094/102199
PISSN	00032670
eISSN	18734324
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.aca.2025.344130
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Analytica Chimica Acta. 2025, 1359, p. 344130
DC.title	Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging
DC.creator	Wee, Wen Ann
	Andini, Tatum Melati
	Kumagai, Tomotaka
	Chinnathambi, Shanmugavel
	Pandian, Ganesh N.
	Millius, Arthur
	Sugiyama, Hiroshi
	Standley, Daron M.
	Park, Soyoung
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-05-03
DC.identifier	https://doi.org/10.1016/j.aca.2025.344130
DC.subject	Fluorescent probe
	LD probe
	Lipid droplets
	Push-pull dye
	Solvatochromism
DC.description	Wee W.A., Andini T.M., Kumagai T., et al. Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging. Analytica Chimica Acta 1359, 344130 (2025); https://doi.org/10.1016/j.aca.2025.344130.
DCTERMS.abstract	Lipid droplets (LDs) are ubiquitous cellular organelles with a neutral lipid core containing triacylglycerols and cholesteryl esters surrounded by phospholipids. Recent findings indicate that LDs are intricately linked to diseases, such as cancer and neurological disorders, in addition to their roles in cellular senescence and immune responses. Herein, we describe a simple yet robust push-pull molecule, N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline (NiTA), as a versatile LD fluorescent probe. NiTA showed an absorption spectrum with a substantial bathochromic shift and a fluorescence spectrum with excellent solvatochromism. Leveraging the remarkable photophysical features of NiTA, we stained LDs in major immune cells, including T and B cells, and macrophages, and monitored the changes in LDs under oxidative and starvation conditions. Furthermore, we demonstrated the applicability of NiTA for visualizing the organization of medaka fish (Oryzias latipes) embryos during development. We expect the small yet powerful NiTA to be utilized in various applications, including fluorescence mapping to observe LD numbers, morphology, and polarity changes in animals and cells.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	102199
citation_title	Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging
citation_author	Wee, Wen Ann
	Andini, Tatum Melati
	Kumagai, Tomotaka
	Chinnathambi, Shanmugavel
	Pandian, Ganesh N.
	Millius, Arthur
	Sugiyama, Hiroshi
	Standley, Daron M.
	Park, Soyoung
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2025-05-03
citation_journal_title	Analytica Chimica Acta
citation_volume	1359
citation_firstpage	344130
citation_doi	https://doi.org/10.1016/j.aca.2025.344130
citation_public_url	https://hdl.handle.net/11094/102199

Tiny but mighty! N,N-dimethyl-4-(5-nitrothiophen-2-yl)aniline, a push-pull fluorescent dye for lipid droplet imaging

Link to primary information

Item Information

Show details