Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity

Fujieda, Kazuki; Sumihiro, Hitoshi; Saito, Rika; Kobayashi, Koki; Miyanohara, Yuki; Abe, Shinya; Tsujii, Toshinori; Tu, Hung Ya; Furukawa, Takahisa

doi:https://doi.org/10.1016/j.bbrc.2025.151966

Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity

Fujieda, Kazuki; Sumihiro, Hitoshi; Saito, Rika et al.

Biochemical and Biophysical Research Communications, 2025, 771, 151966

Number of Access:20（2025-08-19 00:53 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/102201

Link to primary information

File	Format	Terms of use	Size	Views	Date.Available	Description	information
BiochemBiophysResCommun_771	pdf	None	5.96 MB	12	2025.07.10

Creative Commons : Attribution - NonCommercial

Item Information

Output File Export EndNote Basic Export Mendeley

Title	Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity
Creator	CreatorName Fujieda, Kazuki
	CreatorName Sumihiro, Hitoshi
	CreatorName Saito, Rika
	CreatorName Kobayashi, Koki
	CreatorName Miyanohara, Yuki
	CreatorName Abe, Shinya
	CreatorName Tsujii, Toshinori
	NRID 1000010780835 1000010780835 ORCID 0000-0002-4868-6262 0000-0002-4868-6262 researchmap permalink hungyatu hungyatu CreatorName Tu, Hung Ya
	NRID 1000050260609 1000050260609 ORCID 0000-0003-4171-220X 0000-0003-4171-220X researchmap permalink read0083689 read0083689 Scopus Author ID 7403160171 7403160171 CreatorName Furukawa, Takahisa
Description	Fujieda K., Sumihiro H., Saito R., et al. Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity. Biochemical and Biophysical Research Communications 771, 151966 (2025); https://doi.org/10.1016/j.bbrc.2025.151966.
Abstract	In the vertebrate retina, Müller glial cells and neurons originate from common progenitor cells. To identify the genes that regulate the cell fate choice of Müller glial cells from retinal progenitor cells, we first generated Rlbp1-EGFP transgenic mice in which differentiating Müller glia were specifically labeled with EGFP. By performing fluorescence-activated cell sorting and RNA sequencing analysis, we found that Id1, Id2, and Id3, were highly enriched in developing and differentiated Müller glial cells. While Id1/2/3 are inhibitory helix-loop-helix (HLH) transcription factors, the functional role and underlying mechanism of each Id protein in Müller glial cell development are not fully understood. In situ hybridization revealed Id1/2/3 expression in the layer where retinal progenitor cells and Müller glial cells reside in developing and adult retinas, respectively. Cell lineage analysis using retroviruses expressing Id1, Id2, or Id3 showed that Id2 possesses a stronger inducing activity in Müller glial cell fate choice than Id1 and Id3. Co-immunoprecipitation and luciferase reporter analyses suggested that Id2 interacts with the Notch intracellular region and promotes Notch activity. Taken together, these results suggest that Id2 promotes Müller glial cell fate choice by increasing Notch activity in the developing mouse retina.
Publisher	Elsevier B.V.
Source Title	Biochemical and Biophysical Research Communications
Volume (Issue)	771
Page	151966
Date of Issued	2025-05-20
Language	English
Handle URL	https://hdl.handle.net/11094/102201
PISSN	0006291X
eISSN	10902104
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.bbrc.2025.151966
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	Biochemical and Biophysical Research Communications. 2025, 771, p. 151966
DC.title	Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity
DC.creator	Fujieda, Kazuki
	Sumihiro, Hitoshi
	Saito, Rika
	Kobayashi, Koki
	Miyanohara, Yuki
	Abe, Shinya
	Tsujii, Toshinori
	Tu, Hung Ya
	Furukawa, Takahisa
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-05-20
DC.identifier	https://doi.org/10.1016/j.bbrc.2025.151966
DC.description	Fujieda K., Sumihiro H., Saito R., et al. Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity. Biochemical and Biophysical Research Communications 771, 151966 (2025); https://doi.org/10.1016/j.bbrc.2025.151966.
DCTERMS.abstract	In the vertebrate retina, Müller glial cells and neurons originate from common progenitor cells. To identify the genes that regulate the cell fate choice of Müller glial cells from retinal progenitor cells, we first generated Rlbp1-EGFP transgenic mice in which differentiating Müller glia were specifically labeled with EGFP. By performing fluorescence-activated cell sorting and RNA sequencing analysis, we found that Id1, Id2, and Id3, were highly enriched in developing and differentiated Müller glial cells. While Id1/2/3 are inhibitory helix-loop-helix (HLH) transcription factors, the functional role and underlying mechanism of each Id protein in Müller glial cell development are not fully understood. In situ hybridization revealed Id1/2/3 expression in the layer where retinal progenitor cells and Müller glial cells reside in developing and adult retinas, respectively. Cell lineage analysis using retroviruses expressing Id1, Id2, or Id3 showed that Id2 possesses a stronger inducing activity in Müller glial cell fate choice than Id1 and Id3. Co-immunoprecipitation and luciferase reporter analyses suggested that Id2 interacts with the Notch intracellular region and promotes Notch activity. Taken together, these results suggest that Id2 promotes Müller glial cell fate choice by increasing Notch activity in the developing mouse retina.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	102201
citation_title	Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity
citation_author	Fujieda, Kazuki
	Sumihiro, Hitoshi
	Saito, Rika
	Kobayashi, Koki
	Miyanohara, Yuki
	Abe, Shinya
	Tsujii, Toshinori
	Tu, Hung Ya
	Furukawa, Takahisa
citation_publisher	Elsevier B.V.
citation_language	English
citation_date	2025-05-20
citation_journal_title	Biochemical and Biophysical Research Communications
citation_volume	771
citation_firstpage	151966
citation_doi	https://doi.org/10.1016/j.bbrc.2025.151966
citation_public_url	https://hdl.handle.net/11094/102201

Id2 transcriptional repressor promotes retinal Müller glial cell fate choice by increasing Notch activity

Link to primary information

Item Information

Show details