Comparative evaluation of polyhydroxyalkanoates recovery methods for a mixed microbial culture derived from waste activated sludge

Inoue, Daisuke; Miwa, Kohei; Ike, Michihiko

Journal of Material Cycles and Waste Management, 2025, 122478

Number of Access:11（2025-07-31 22:37 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/102253

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Title
Comparative evaluation of polyhydroxyalkanoates recovery methods for a mixed microbial culture derived from waste activated sludge
Creator
NRID
1000070448091 1000070448091
ORCID
0000-0002-9216-8198 0000-0002-9216-8198
researchmap permalink
DaisukeINOUE DaisukeINOUE
Scopus Author ID
55723196300 55723196300
CreatorName
Inoue, Daisuke
CreatorName
Miwa, Kohei
NRID
1000040222856 1000040222856
ORCID
0000-0001-6237-8103 0000-0001-6237-8103
researchmap permalink
ikemichi ikemichi
Scopus Author ID
55551254800 55551254800
CreatorName
Ike, Michihiko
Subject
Downstream processing
Mixed microbial culture
Polyhydroxyalkanoate
Recovery
Waste activated sludge
Description
The version of record of this article, first published in Journal of Material Cycles and Waste Management, is available online at Publisher’s website: https://doi.org/10.1007/s10163-025-02242-3.
Abstract
The production of polyhydroxyalkanoates (PHAs) using a mixed microbial culture (MMC) derived from waste activated sludge (WAS) is a promising strategy for converting wastewater treatment plants into biorefinery facilities. Despite many studies on PHA production, the recovery of PHA stored in WAS-derived MMC has received less attention. In this study, we comparatively evaluated three solvent extraction, three cellular digestion, and two mechanical disruption methods to determine their effectiveness in the recovery of PHA from WAS-derived MMC. Chloroform extraction, sodium hydroxide digestion, and ultrasonic disruption were selected as effective candidates in initial screenings, and their processing conditions were optimized. While PHA recovery by the optimized chloroform extraction was 74%, the recovered polymers contained ignorable impurities and had superior molecular properties. In contrast, sodium hydroxide digestion and ultrasonic disruption enabled PHA recovery without loss, but the purity (> 73% and ≥ 80%, respectively) and molecular properties of the recovered polymers required some improvement. Our findings suggest that these PHA recovery methods would be applicable in PHA production using WAS-derived MMC with pre- or post-processing as necessary, though they did not necessarily satisfy all the requirements in PHA production systems.
Publisher
Springer
Source Title
Journal of Material Cycles and Waste Management
Page
122478
Date of Issued
2025-05-12
Language
English
Handle URL
https://hdl.handle.net/11094/102253
PISSN
14384957
eISSN
16118227
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1007/s10163-025-02242-3
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article