Mapping of projection of sprouting neuron in neonate and adult mice after pyramidotomy

Tsujioka, Hiroshi; Yamashita, Toshihide

Experimental Neurology, 2025, 393, 115386

Number of Access:165（2026-04-04 15:18 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/102776

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Title
Mapping of projection of sprouting neuron in neonate and adult mice after pyramidotomy
Creator
NRID
1000020803505 1000020803505
ORCID
0000-0001-7484-4191 0000-0001-7484-4191
researchmap permalink
tsujioka tsujioka
Scopus Author ID
57188553938 57188553938
CreatorName
Tsujioka, Hiroshi
NRID
1000010301269 1000010301269
ORCID
0000-0003-4559-7018 0000-0003-4559-7018
researchmap permalink
ToshihideYamashita ToshihideYamashita
Scopus Author ID
35479670200 35479670200
CreatorName
Yamashita, Toshihide
Subject
Corticospinal tract
MAPseq
Neonate
Pyramidotomy
Sprouting
Description
Tsujioka H., Yamashita T.. Mapping of projection of sprouting neuron in neonate and adult mice after pyramidotomy. Experimental Neurology 393, 115386 (2025); https://doi.org/10.1016/j.expneurol.2025.115386.
Abstract
Although severed axons of the corticospinal tract rarely regenerate, neural function is partially recovered by axonal sprouting from intact neurons. The sprouting capability is higher in neonates than in adults, making neonates an attractive model for finding novel therapeutic targets for central nervous system injury. The axonal projection pattern of the motor cortex neurons is highly heterogeneous, and high-throughput analysis at the single-cell level is required to correctly understand the projection pattern of sprouting neurons. The recently developed multiplexed analysis of projections by sequencing (MAPseq) has revealed axonal projection complexity in many brain areas; however, axonal sprouting of the corticospinal tract in neonates and adults has not yet been analyzed. Here, we evaluated the sprouting pattern of individual neurons using MAPseq in neonate and adult mice after pyramidotomy, using the intact and denervated (sprouting) sides of the cervical cord. The ratio of neurons projecting solely to the intact or denervated side or projecting to both sides was not significantly different. The cumulative projection strength on the denervated side was significantly higher in the neonatal pyramidotomy group than in the other groups. The ratio of the projection strength on the denervated side to the total projection strength (sprouting index) correlated with the projection strength on the denervated side. The ratio of the neuron with a sprouting index of 0.6–0.7 was significantly higher in the neonatal pyramidotomy group. These results show the usefulness of MAPseq in sprouting studies and provide important information regarding the sprouting patterns of individual neurons in neonates.
Publisher
Elsevier Inc.
Source Title
Experimental Neurology
Volume (Issue)
393
Page
115386
Date of Issued
2025-11-01
Language
English
Handle URL
https://hdl.handle.net/11094/102776
PISSN
00144886
eISSN
10902430
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1016/j.expneurol.2025.115386
Access Rights
open access
CopyRight
This article is licensed under a Creative Commons Attribution 4.0 International License.
© 2025 The Authors
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article