Raman flow cytometry using time-delay integration

Lindley, Matthew; Kubo, Toshiki; Devineau, Stéphanie et al.

Optica, 2025, 12(4), 479-489

Number of Access:11（2025-11-24 14:19 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/102906

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Title
Raman flow cytometry using time-delay integration
Creator
CreatorName
Lindley, Matthew
NRID
1000050975159 1000050975159
ORCID
0000-0002-5696-4111 0000-0002-5696-4111
researchmap permalink
kubo_toshiki_8 kubo_toshiki_8
CreatorName
Kubo, Toshiki
ORCID
0000-0002-1133-5223 0000-0002-1133-5223
Scopus Author ID
55598058400 55598058400
CreatorName
Devineau, Stéphanie
CreatorName
Li, Menglu
CreatorName
Qiao, Jing
NRID
1000000726482 1000000726482
ORCID
0000-0002-9319-3467 0000-0002-9319-3467
researchmap permalink
takuya846 takuya846
Scopus Author ID
7007120787 7007120787
CreatorName
Yashiro, Takuya
NRID
1000020314510 1000020314510
ORCID
0000-0002-4394-7267 0000-0002-4394-7267
researchmap permalink
shirohiwanaga shirohiwanaga
Scopus Author ID
57200822888 57200822888
CreatorName
Iwanaga, Shiroh
NRID
1000090468489 1000090468489
ORCID
0000-0002-7377-1303 0000-0002-7377-1303
researchmap permalink
kazuyomoro kazuyomoro
Scopus Author ID
16638841900 16638841900
CreatorName
Moro, Kazuyo
NRID
1000080362664 1000080362664
ORCID
0000-0002-2284-375X 0000-0002-2284-375X
researchmap permalink
read0089911 read0089911
Scopus Author ID
7404058829 7404058829
CreatorName
Fujita, Katsumasa
Description
Lindley M., Kubo T., Devineau S., et al. Raman flow cytometry using time-delay integration. Optica 12, 479 (2025); https://doi.org/10.1364/OPTICA.551480.
Abstract
Raman flow cytometry offers chemically sensitive, label-free measurement of cells and particles; however, the technique suffers from low cell throughput due to the weak Raman signal. Here, we demonstrate the use of time-delay integration (TDI) to achieve Raman flow cytometry combined with dual-sided line illumination. The use of line illumination from both sides of the cell flow capillary kept the cell stream in the detection area by balancing optical force from the illumination lines. The TDI allowed the accumulation of Raman signals from flowing cells without sacrificing the spectrum readout rate. With the developed system, we achieved Raman flow cytometry at throughputs of 32 and 78 events per second for cell and particle detection, respectively. We applied the technique for analyzing biological cells and successfully detected lipid uptake in HepG2 cells and degranulation in bone-marrow-derived murine mast cells. Our TDI Raman flow cytometry approach improves the throughput of Raman-spectroscopy-based cell analysis and extends its applicability to a wider range of biomedical research.
Publisher
Optica Publishing Group (formerly OSA)
Source Title
Optica
Volume (Issue)
12(4)
Page
479-489
Date of Issued
2025-04-02
Language
English
Handle URL
https://hdl.handle.net/11094/102906
PISSN
23342536
Relation.isIdenticalTo
DOI (Publisher Version)
https://doi.org/10.1364/OPTICA.551480
Access Rights
open access
CopyRight
© 2025 Optica Publishing Group. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
oaire:version
Version of Record
Category
学術雑誌論文 Journal Article