Development of a peptide-based vaccine using a T cell epitope derived from SARS-CoV-2

Baba, Satoshi; Hayashi, Hiroki; Yoshida, Shota; Yin, Nanxiang; Shimamura, Munehisa; Morishita, Ryuichi; Rakugi, Hiromi; Nakagami, Hironori; Yamamoto, Koichi

doi:https://doi.org/10.1016/j.isci.2025.112542

Development of a peptide-based vaccine using a T cell epitope derived from SARS-CoV-2

Baba, Satoshi; Hayashi, Hiroki; Yoshida, Shota et al.

iScience, 2025, 28(6), 112542

Number of Access:10（2025-11-07 08:47 Counts）

Identifier to cite or link to this item: https://hdl.handle.net/11094/102910

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Title	Development of a peptide-based vaccine using a T cell epitope derived from SARS-CoV-2
Creator	CreatorName Baba, Satoshi
	NRID 1000020813364 1000020813364 researchmap permalink hayashi-hiro hayashi-hiro Scopus Author ID 55702932900 55702932900 CreatorName Hayashi, Hiroki
	CreatorName Yoshida, Shota
	CreatorName Yin, Nanxiang
	NRID 1000060422317 1000060422317 ORCID 0000-0003-0374-0787 0000-0003-0374-0787 researchmap permalink read8359 read8359 Scopus Author ID 7102059307 7102059307 CreatorName Shimamura, Munehisa
	NRID 1000040291439 1000040291439 researchmap permalink read0051652 read0051652 Scopus Author ID 7102241494 7102241494 CreatorName Morishita, Ryuichi
	NRID 1000020252679 1000020252679 ORCID 0000-0001-6508-4338 0000-0001-6508-4338 researchmap permalink read0042763 read0042763 Scopus Author ID 57202699453 57202699453 CreatorName Rakugi, Hiromi
	NRID 1000020325369 1000020325369 ORCID 0000-0003-4494-3601 0000-0003-4494-3601 researchmap permalink hironorinakagami hironorinakagami Scopus Author ID 7006290279 7006290279 CreatorName Nakagami, Hironori
	NRID 1000000528424 1000000528424 ORCID 0000-0003-0107-9315 0000-0003-0107-9315 researchmap permalink kymt0410 kymt0410 Scopus Author ID 55624482466 55624482466 CreatorName Yamamoto, Koichi
Subject	Immunology
Subject	Virology
Description	Baba S., Hayashi H., Yoshida S., et al. Development of a peptide-based vaccine using a T cell epitope derived from SARS-CoV-2. iScience 28, 112542 (2025); https://doi.org/10.1016/j.isci.2025.112542.
Abstract	Follicular helper T (Tfh) cells are a subset of CD4+ T cells that help B cells to produce high-affinity antibodies. Efficient Tfh cell induction by vaccines is critical for protective efficacy against diseases. A Tfh epitope, identified from the SARS-CoV-2 spike protein, has been shown to bind to the corresponding HLA and activates Tfh cells. Here, we assessed the efficacy of a peptide vaccine conjugated with SARS-CoV-2 Tfh epitope against experimental hypertension in a mouse model. The Tfh-angiotensin II (Tfh-Ang II) vaccine activated Tfh and germinal center B cells and induced antibodies against Ang II, thereby suppressing hypertension. However, Ang II-specific autoreactive T cells were not induced. Interestingly, Tfh-Ang II-induced antibody production was enhanced by SARS-CoV-2 spike priming. Moreover, human peripheral blood mononuclear cells from individuals vaccinated with COVID-19 mRNA vaccine were activated by Tfh epitope. Collectively, the SARS-CoV-2 spike-derived universal Tfh epitope may be effective for peptide-based vaccine development.
Publisher	Elsevier Inc.
Source Title	iScience
Volume (Issue)	28(6)
Page	112542
Date of Issued	2025-05-13
Language	English
Handle URL	https://hdl.handle.net/11094/102910
eISSN	25890042
Relation.isIdenticalTo	DOI (Publisher Version) https://doi.org/10.1016/j.isci.2025.112542
Access Rights	open access
CopyRight	This article is licensed under a Creative Commons Attribution 4.0 International License.
oaire:version	Version of Record
Category	学術雑誌論文 Journal Article

Resource Type	journal article
Local Type	Journal Article
DCMI Type	Text
DCTERMS.bibliographicCitation	iScience. 2025, 28(6), p. 112542
DC.title	Development of a peptide-based vaccine using a T cell epitope derived from SARS-CoV-2
DC.creator	Baba, Satoshi
	Hayashi, Hiroki
	Yoshida, Shota
	Yin, Nanxiang
	Shimamura, Munehisa
	Morishita, Ryuichi
	Rakugi, Hiromi
	Nakagami, Hironori
	Yamamoto, Koichi
DC.publisher	Elsevier Inc.
DC.language' scheme='DCTERMS.RFC1766	English
DC.type' scheme='DCTERMS.DCMIType	Text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2025-05-13
DC.identifier	https://doi.org/10.1016/j.isci.2025.112542
DC.subject	Immunology
DC.subject	Virology
DC.description	Baba S., Hayashi H., Yoshida S., et al. Development of a peptide-based vaccine using a T cell epitope derived from SARS-CoV-2. iScience 28, 112542 (2025); https://doi.org/10.1016/j.isci.2025.112542.
DCTERMS.abstract	Follicular helper T (Tfh) cells are a subset of CD4+ T cells that help B cells to produce high-affinity antibodies. Efficient Tfh cell induction by vaccines is critical for protective efficacy against diseases. A Tfh epitope, identified from the SARS-CoV-2 spike protein, has been shown to bind to the corresponding HLA and activates Tfh cells. Here, we assessed the efficacy of a peptide vaccine conjugated with SARS-CoV-2 Tfh epitope against experimental hypertension in a mouse model. The Tfh-angiotensin II (Tfh-Ang II) vaccine activated Tfh and germinal center B cells and induced antibodies against Ang II, thereby suppressing hypertension. However, Ang II-specific autoreactive T cells were not induced. Interestingly, Tfh-Ang II-induced antibody production was enhanced by SARS-CoV-2 spike priming. Moreover, human peripheral blood mononuclear cells from individuals vaccinated with COVID-19 mRNA vaccine were activated by Tfh epitope. Collectively, the SARS-CoV-2 spike-derived universal Tfh epitope may be effective for peptide-based vaccine development.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	102910
citation_title	Development of a peptide-based vaccine using a T cell epitope derived from SARS-CoV-2
citation_author	Baba, Satoshi
	Hayashi, Hiroki
	Yoshida, Shota
	Yin, Nanxiang
	Shimamura, Munehisa
	Morishita, Ryuichi
	Rakugi, Hiromi
	Nakagami, Hironori
	Yamamoto, Koichi
citation_publisher	Elsevier Inc.
citation_language	English
citation_date	2025-05-13
citation_journal_title	iScience
citation_volume	28
citation_issue	6
citation_firstpage	112542
citation_doi	https://doi.org/10.1016/j.isci.2025.112542
citation_public_url	https://hdl.handle.net/11094/102910

Development of a peptide-based vaccine using a T cell epitope derived from SARS-CoV-2

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